Grin1^tm1Mim
Targeted Allele Detail

Summary

• Symbol: Grin1^tm1Mim
• Name: glutamate receptor, ionotropic, NMDA1 (zeta 1); targeted mutation 1, Masayoshi Mishina
• MGI ID: MGI:3833550
• Synonyms: GluRzeta1^flox, NR1^flox
• Gene: Grin1 Location: Chr2:25181193-25209199 bp, - strand Genetic Position: Chr2, 17.14 cM
• Alliance: Grin1^tm1Mim page

Increased susceptibility to kainate-induced tonic-clonic seizures in Grin1^tm1Mim/Grin1^tm1Mim Grik4^tm1(cre)Mim/Grik4⁺ mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144994
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A loxP site was inserted between exons 18 and 19 and a 1.8kb DNA fragment containing another loxP site as well as an frt flanked PGK-neomycin selection cassette was inserted between exons 20 and 21. Mice were crossed to Tg(EIF1A-flp)66Mim to remove the neomycin selection cassette. Exons 19 and 20 remain flanked by loxP sites. (J:144994)

Generation of the Grin1^tm1Mim allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grin1 Mutation: 64 strains or lines available

References

• Original: J:144994 Fukushima F, et al., Ablation of NMDA receptors enhances the excitability of hippocampal CA3 neurons. PLoS One. 2009;4(1):e3993
• All: 3 reference(s)