Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Opn4tm1.1(cre)Saha mutation
(0 available);
any
Opn4 mutation
(33 available)
Pou4f2tm4(DTA)Whk mutation
(1 available);
any
Pou4f2 mutation
(7 available)
Tg(CAG-Bgeo/ALPP)1Lbe mutation
(1 available)
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nervous system
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• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls
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• innervation of the intergeniculate leaflet (IGL) is markedly reduced
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• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2tm2.1Nat/+ Opn4tm1.1(cre)Saha/+ animals
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vision/eye
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• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2tm2.1Nat/+ Opn4tm1.1(cre)Saha/+ animals
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Opn4tm1.1(cre)Saha mutation
(0 available);
any
Opn4 mutation
(33 available)
Pou4f2tm4(DTA)Whk mutation
(1 available);
any
Pou4f2 mutation
(7 available)
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behavior/neurological
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N |
• mutants are able to photoentrain (their circadian phase) like controls; also, activity
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• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR
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• animals show a minor deficit in period lengthening under constant light conditions
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nervous system
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• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas
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vision/eye
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N |
• visual acuity is comparable to wild-type animals
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• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR
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• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Opn4tm1.1(cre)Saha mutation
(0 available);
any
Opn4 mutation
(33 available)
Opn4tm1Yau mutation
(0 available);
any
Opn4 mutation
(33 available)
Pou4f2tm4(DTA)Whk mutation
(1 available);
any
Pou4f2 mutation
(7 available)
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nervous system
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• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls
• no fibres are observed in the shell of the OPN
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• innervation of the intergeniculate nucleus (IGL) is markedly reduced
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• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2tm2.1Nat/+ ;Opn4tm1.1(cre)Saha/+ animals
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vision/eye
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• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2tm2.1Nat/+ ;Opn4tm1.1(cre)Saha/+ animals
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pou4f2tm4(DTA)Whk mutation
(1 available);
any
Pou4f2 mutation
(7 available)
Tg(CAG-cre/Esr1*)5Amc mutation
(9 available)
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vision/eye
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• reduced cross-sectional area by 48.8% in 3 months after a 5 day course of tamoxifen treatment
• cross-sectional area reduced to 19.7% of controls at ten months after a 5 day course of tamoxifen treatment
• axons disorganized
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• progressive degeneration over the course of ten months after a 5 day course of tamoxifen treatment
• severe axon degeneration and vacuolation
• empty or abnormal myelin sheaths
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• 60% reduction in the number of cells of the ganglion layer one month after a 5 day course of tamoxifen treatment
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• 33% of time spent in the light as opposed to 6% for controls after 1-2 hours of dark adaptation
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nervous system
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• reactive gliosis triggered one month after a 5 day course of tamoxifen treatment
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• reduced cross-sectional area by 48.8% in 3 months after a 5 day course of tamoxifen treatment
• cross-sectional area reduced to 19.7% of controls at ten months after a 5 day course of tamoxifen treatment
• axons disorganized
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• progressive degeneration over the course of ten months after a 5 day course of tamoxifen treatment
• severe axon degeneration and vacuolation
• empty or abnormal myelin sheaths
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pou4f2tm4(DTA)Whk mutation
(1 available);
any
Pou4f2 mutation
(7 available)
Tg(Six3-cre)69Frty mutation
(2 available)
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vision/eye
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• clearly abnormal in 16 day old mice
• thinner with smaller diameter fibers
• unmyelinated fibers
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• overall structure remains normal through 5 months of age
• reduced cell proliferation and increased apoptosis
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• more than 98% of retinal ganglion cells ablated at E14.5
• only displaced amacrine cells present at 16 days of age
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• noticeably thinner at E14.5
• 30-50% thinner than controls at 16 days of age
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• saturated amplitudes of both light and dark adapted b-waves 25% of normal
• dark adapted a-wave amplitude 42% of normal
• negative scotopic threshold response is very small
• positive scotopic threshold response is absent
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nervous system
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• clearly abnormal in 16 day old mice
• thinner with smaller diameter fibers
• unmyelinated fibers
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Analysis Tools