Phenotypes associated with this allele

• Allele Symbol: Knk
• Allele Name: kinked tail
• Allele ID: MGI:3834126
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Knk/Knk	involves: C57BL/6J * FVB/N	MGI:4412366
ht2	Knk/Knk^+	involves: C57BL/6J * FVB/N	MGI:4412365

Genotype
MGI:4412366

hm1

• Allelic Composition: Knk/Knk
• Genetic Background: involves: C57BL/6J * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and placentation, complete penetrance ( J:154368 )

• very few survive to E8.5 and none are found in decidua past E8.5

embryo

failure to gastrulate ( J:154368 )

• expression analysis indicates that embryos fail to undergo gastrulation

embryonic growth retardation ( J:154368 )

• little growth occurs between E6.5 and E7.5

decreased embryo size ( J:154368 )

• at E7.5 mutant embryos are consistently smaller than their normal littermates and are approximately the size of a normal E6.5

abnormal embryonic tissue morphology ( J:154368 )

• smaller embryonic portion at E6.5

abnormal embryonic epiblast morphology ( J:154368 )

• epiblast dysmorphogenesis

absent mesoderm ( J:154368 )

• at E7.5 embryos have small, grossly malformed embryonic portions with no apparent mesoderm

disorganized embryonic tissue ( J:154368 )

• at E6.5

abnormal Reichert's membrane morphology ( J:154368 )

• at E7.5, Reicherts' membrane often appears to be loosely adhered to the egg cylinder

abnormal visceral yolk sac morphology ( J:154368 )

• at E7.5, the yolk sac often appears to be loosely adhered to the egg cylinder

abnormal anterior visceral endoderm morphology ( J:154368 )

• at E6.5 cells maintain a cuboidal morphology around the entire egg cylinder and the amniotic cavity is only beginning to form

• at E7.5 cells maintain a cuboidal morphology and are not organized lack mature AVE

abnormal distal visceral endoderm morphology ( J:154368 )

• failure of the distal visceral endoderm to form the anterior visceral endoderm

• expression analysis indicates the distal visceral endoderm is formed but timing of migration is abnormal and cells fail to adopt the correct morphology

growth/size/body

embryonic growth retardation ( J:154368 )

• little growth occurs between E6.5 and E7.5

decreased embryo size ( J:154368 )

• at E7.5 mutant embryos are consistently smaller than their normal littermates and are approximately the size of a normal E6.5

cellular

abnormal basement membrane morphology ( J:154368 )

• at E6.5 discontinuous laminin localization in embryos suggests a defect in basement membrane morphology

Genotype
MGI:4412365

ht2

• Allelic Composition: Knk/Knk⁺
• Genetic Background: involves: C57BL/6J * FVB/N

limbs/digits/tail

abnormal caudal vertebrae morphology ( J:154368 )

• display wedge, block, and hemi-vertebrae

caudal hemivertebra ( J:154368 )

caudal vertebral fusion ( J:154368 )

decreased caudal vertebrae number ( J:154368 )

abnormal tail development ( J:154368 )

• occasional fusion of the myotomes in the tail

• tail kink first detected at E13.5 as a curled tail tip

short tail ( J:154368 )

• variations in tail length are due to a reduced number of total vertebrae, severe malformation of vertebrae (i.e., fused or wedge-shaped vertebrae), and/or a combination of the two

• tails range from almost full length with a few kinks to a curly Q tail

kinked tail ( J:154368 )

• variable kinkyness, form a few kinks to a curly Q tail

• tail kink first detected at E13.5 as a curled tail tip

skeleton

abnormal caudal vertebrae morphology ( J:154368 )

• display wedge, block, and hemi-vertebrae

caudal hemivertebra ( J:154368 )

caudal vertebral fusion ( J:154368 )

decreased caudal vertebrae number ( J:154368 )

abnormal intervertebral disk morphology ( J:154368 )

• variably sized or absent intervertebral disks in the tail

embryo

abnormal notochord morphology ( J:154368 )

• dorsally branched and thickened notochord at E12.5

• abnormalities are restricted to the tail region

split notochord ( J:154368 )

muscle

abnormal myotome development ( J:154368 )

• occasional fusion of the myotomes in the tail