Phenotypes associated with this allele

• Allele Symbol: Slc1a3^{tm1(cre/ERT2)Mgoe}
• Allele Name: targeted mutation 1, Magdalena Goetz
• Allele ID: MGI:3830051
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor Pten^tm1Mro/Pten^tm1Mro Slc1a3^tm1(cre/ERT2)Mgoe/Slc1a3^+ Trp53^tm1Brn/Trp53^tm1Brn	involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJaeSor	MGI:5790976
cn2	Ntrk2^tm2Kln/Ntrk2^tm2Kln Slc1a3^tm1(cre/ERT2)Mgoe/? Gt(ROSA)26Sor^tm1Sor/?	involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * SJL	MGI:3830091
cn3	Chd7^tm2c(EUCOMM)Wtsi/Chd7^tm2c(EUCOMM)Wtsi Slc1a3^tm1(cre/ERT2)Mgoe/Slc1a3^+	involves: 129S2/SvPas * C57BL/6 * C57BL/6N * SJL	MGI:7496108
cn4	Ntrk2^tm2Kln/Ntrk2^tm2Kln Slc1a3^tm1(cre/ERT2)Mgoe/? Tg(CAG-Bgeo/GFP)21Lbe/?	involves: 129/Sv * C57BL/6 * SJL	MGI:3830092

Genotype
MGI:5790976

cn1

• Allelic Composition: Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor; Pten^tm1Mro/Pten^tm1Mro; Slc1a3^{tm1(cre/ERT2)Mgoe}/Slc1a3⁺; Trp53^tm1Brn/Trp53^tm1Brn
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJaeSor

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased glioma incidence ( J:229481 )

• 3 of 16 mice injected with endoxifen develop glial tumors with lesions of different sizes and varying extents of infiltration

• small neoplastic lesions in endoxifen-induced mice arise from beneath the SVZ and extend into the striatum and dorsally into the corpus callosum

• larger tumors of endoxifen-induced mice show expansion into and a diffuse infiltration of the entire caudate nucleus

• some tumors contain areas of necrosis and occasional microvascular proliferations similar to those in malignant gliomas

increased astrocytoma incidence ( J:229481 )

• endoxifen-induced tumors show glial morphology with features typical of astrocytomas and oligodendrogliomas

increased oligodendroglioma incidence ( J:229481 )

• endoxifen-induced tumors show glial morphology with features typical of astrocytomas and oligodendrogliomas

nervous system

increased glioma incidence ( J:229481 )

• 3 of 16 mice injected with endoxifen develop glial tumors with lesions of different sizes and varying extents of infiltration

• small neoplastic lesions in endoxifen-induced mice arise from beneath the SVZ and extend into the striatum and dorsally into the corpus callosum

• larger tumors of endoxifen-induced mice show expansion into and a diffuse infiltration of the entire caudate nucleus

• some tumors contain areas of necrosis and occasional microvascular proliferations similar to those in malignant gliomas

increased astrocytoma incidence ( J:229481 )

• endoxifen-induced tumors show glial morphology with features typical of astrocytomas and oligodendrogliomas

increased oligodendroglioma incidence ( J:229481 )

• endoxifen-induced tumors show glial morphology with features typical of astrocytomas and oligodendrogliomas

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
high grade glioma		DOID:3070	OMIM:PS137800	J:229481

Genotype
MGI:3830091

cn2

• Allelic Composition: Ntrk2^tm2Kln/Ntrk2^tm2Kln; Slc1a3^{tm1(cre/ERT2)Mgoe}/?; Gt(ROSA)26Sor^tm1Sor/?
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * SJL

nervous system

abnormal neuron differentiation ( J:143452 )

• fewer neurons arising in hippocampus from radial glial cells after treatment with tamoxifen survive to become mature neurons

• axon elongation is unaffected

abnormal dendrite morphology ( J:143452 )

• dendritic branching and lengths are reduced in neurons arising in the hippocampus from radial glial cells after treatment with tamoxifen

• reduced dendritic complexity 90 um from soma

• spine density is significantly reduced

reduced long-term potentiation ( J:143452 )

• lasts only 30-40 minutes as compared to over 90 minutes for controls

behavior/neurological

increased thigmotaxis ( J:143452 )

• studied between 28 and 42 days after tamoxifen treatment

• enter the center in an open field test less frequently and spend less time there

• more time spent on the periphery in an open field test

• in an elevated + maze, fewer entries into the open arms and less time spent in the open arms

decreased locomotor activity ( J:143452 )

• studied between 28 and 42 days after tamoxifen treatment

• less spontaneous activity in an open field test

cellular

abnormal neuron differentiation ( J:143452 )

• fewer neurons arising in hippocampus from radial glial cells after treatment with tamoxifen survive to become mature neurons

• axon elongation is unaffected

Genotype
MGI:7496108

cn3

• Allelic Composition: Chd7^{tm2c(EUCOMM)Wtsi}/Chd7^{tm2c(EUCOMM)Wtsi}; Slc1a3^{tm1(cre/ERT2)Mgoe}/Slc1a3⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * C57BL/6N * SJL

nervous system

increased neuron apoptosis ( J:222062 )

• in the hippocampus at 12 weeks after tamoxifen treatment

abnormal neuronal stem cell morphology ( J:222062 )

• at 8 months after tamoxifen injection there is a significant depletion of neuronal stem cells

abnormal neuron number ( J:222062 )

decreased neuronal precursor cell number ( J:222062 )

• at 12 weeks after tamoxifen treatment a significant decrease in the number of neuronal progenitors and immature neurons

increased neuronal precursor cell number ( J:222062 )

• increase in the number of progenitors and immature neurons 4 weeks after tamoxifen injection

• at 12 weeks after tamoxifen treatment radial neuronal stem cells numbers are increased almost 3-fold and the number of proliferating radial neuronal stem cells is increased over 2-fold

decreased neuron number ( J:222062 )

• at 12 weeks and 8 months after tamoxifen treatment a significant decrease in the number of neuronal progenitors and immature neurons is seen

increased neuron number ( J:222062 )

• increase in the number of progenitors and immature neurons 4 weeks after tamoxifen injection

increased neuronal stem cell self-renewal ( J:222062 )

• increase in proliferation in the subgranular zone 6-7 days after following tamoxifen injection

cellular

increased neuron apoptosis ( J:222062 )

• in the hippocampus at 12 weeks after tamoxifen treatment

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
CHARGE syndrome		DOID:0050834	OMIM:214800	J:222062

Genotype
MGI:3830092

cn4

• Allelic Composition: Ntrk2^tm2Kln/Ntrk2^tm2Kln; Slc1a3^{tm1(cre/ERT2)Mgoe}/?; Tg(CAG-Bgeo/GFP)21Lbe/?
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL

nervous system

nervous system phenotype ( J:143452 )

N • synaptic contacts appear to be normal for neurons arising in hippocampus from radial glial cells after treatment with tamoxifen