Phenotypes associated with this allele

• Allele Symbol: Tg(Scx-GFP)1Stzr
• Allele Name: transgene insertion 1, Ronen Schweitzer
• Allele ID: MGI:3717419
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tbx5^tm1Jse/Tbx5^tm1Jse Tg(Prrx1-cre/ERT2)1Mlgn/0 Tg(Scx-GFP)1Stzr/0	Not Specified	MGI:4442422
cn2	Runx3^tm3Yg/Runx3^tm3Yg Tg(Scx-GFP)1Stzr/0	Not Specified	MGI:7281800
cx3	Scx^tm1.1Stzr/Scx^tm1.1Stzr Tg(Scx-GFP)1Stzr/0	Not Specified	MGI:3717427
tg4	Tg(Scx-GFP)1Stzr/Tg(Scx-GFP)1Stzr	Not Specified	MGI:3717422

Genotype
MGI:4442422

cn1

• Allelic Composition: Tbx5^tm1Jse/Tbx5^tm1Jse; Tg(Prrx1-cre/ERT2)1Mlgn/0; Tg(Scx-GFP)1Stzr/0
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

abnormal tendon morphology ( J:157917 )

• some tendon fibers connecting forearm muscles to skeletal elements in the handplate are thinner than in controls and some have fused together

• in forelimb, tendons develop functional entheses on the forming skeleton

limbs/digits/tail

abnormal forelimb morphology ( J:157917 )

• with tamoxifen treatment at E9.5, limbs display minor skeletal deformities similar to those in humans with Holt-Oram syndrome (triphalangeal thumb)

• muscle patterning is disrupted similarly to that with tamoxifen treatment at E10.5

• patterning of tendon fibers connecting forearm muscles to skeletal elements in the handplate is significantly disrupted compared to wild-type; fewer tendon fibers are present

• mispatterned muscles make functional myotendinous attachments to tendons

abnormal limb development ( J:157917 )

skeleton

abnormal tendon morphology ( J:157917 )

• some tendon fibers connecting forearm muscles to skeletal elements in the handplate are thinner than in controls and some have fused together

• in forelimb, tendons develop functional entheses on the forming skeleton

Genotype
MGI:7281800

cn2

• Allelic Composition: Runx3^tm3Yg/Runx3^tm3Yg; Tg(Scx-GFP)1Stzr/0
• Genetic Background: Not Specified

skeleton

skeleton phenotype ( J:243559 )

• do not display scoliosis at 3 months of age

Genotype
MGI:3717427

cx3

• Allelic Composition: Scx^tm1.1Stzr/Scx^tm1.1Stzr; Tg(Scx-GFP)1Stzr/0
• Genetic Background: Not Specified

muscle

abnormal tendon morphology ( J:122742 )

• limb tendons are lost with some missing completely and others small and rudimentary

• the extensor digitorium communis tendon ends are the wrist

• the extensor digiti quinti and carpi ulnaris tendons are reduced

• flexor tendons are more severely affected than extensors

• tail tendons are missing

• short tendons in the trunk are reduced or disrupted while long tendons are missing

• intermuscular tendons including the lateral tendious stripes in the rectus abdominis muscle, subscapularis musclea at the ventral side of the scapula, and the middle tendon of the diaphragm can not be detected

• however, anchoring tendons are normal and mice are mobile

behavior/neurological

abnormal tail movements ( J:122742 )

• mice cannot use their tails

limbs/digits/tail

abnormal tail morphology ( J:122742 )

• tail tendons are missing

skeleton

abnormal tendon morphology ( J:122742 )

• limb tendons are lost with some missing completely and others small and rudimentary

• the extensor digitorium communis tendon ends are the wrist

• the extensor digiti quinti and carpi ulnaris tendons are reduced

• flexor tendons are more severely affected than extensors

• tail tendons are missing

• short tendons in the trunk are reduced or disrupted while long tendons are missing

• intermuscular tendons including the lateral tendious stripes in the rectus abdominis muscle, subscapularis musclea at the ventral side of the scapula, and the middle tendon of the diaphragm can not be detected

• however, anchoring tendons are normal and mice are mobile

Genotype
MGI:3717422

tg4

• Allelic Composition: Tg(Scx-GFP)1Stzr/Tg(Scx-GFP)1Stzr
• Genetic Background: Not Specified

normal phenotype

no abnormal phenotype detected ( J:121561 )

• mice are viable with no overt phenotype