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Allele Symbol Allele Name Allele ID |
Map2k1tm1Chrn targeted mutation 1, Jean Charron MGI:3714918 |
| Summary |
10 genotypes |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre), mutants show 50% survival at 44 weeks compared to 37.5 weeks in single Kras heterozygous controls, indicating a slight 22% increase in survival
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• Ad-Cre treated mice show 50% survival at 57 weeks of age compared to 33 weeks of age in single Kras heterozygous controls, indicating an almost 100% increase in survival
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• 6 months following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre), only a few tumors are observed; tumors that are present carry unrecombined Map2k1 alleles and express normal levels of Map2k1
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• males show no significant differences in testis weight, seminiferous tubule diameter, seminiferous epithelium morphology, expression of Sertoli and germ cell markers, total epididymal sperm count or sperm motility relative to control males
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• seminal vesicle weight is significantly reduced at P75 and P180
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• although an increase in Leydig cell numbers is observed up to ~P35, the total number of Leydig cells (Cyp11a1 positive cells) per testis is significantly reduced at all ages examined
• Leydig cell hypoplasia is accompanied by reduced testicular expression of several Leydig cell markers
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• in culture, primary Leydig cells from 50-day-old males show a drastic reduction in basal testosterone as well as severely impaired testosterone synthesis in response to stimulation with hCG (human choriogonadotropin), dibutyryl(Bt2)cAMP (a permeable cAMP analog), 22OHC (22-hydroxycholesterol) or P5 (pregnenolone)
• however, cAMP accumulation in response to hCG stimulation is normal
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• when males are paired with females of known fertility, the average number of pups/littler is significantly lower than that for control males (3.3 +/- 1.9 versus 6.5 +/- 0.6, respectively)
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• male fertility starts to decline at ~3 months of age, and by 6 months the cumulative number of pups sired by males paired with fertile females is only ~50% of that sired by control males
• at the end of the 6-month mating period, the number of litters and number of pups/litter are significantly reduced relative to controls from 5.7 +/- 0.2 to 3.8 +/- 0.6 and from 6.2 +/- 0.3 to 4.8 +/- 0.5, respectively, while the number of days between litters is increased from 27 +/- 1 to 31 +/- 1
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• at P180, males exhibit several signs of hypoandrogenemia, including increased serum LH levels, reduced expression of two renal androgen-responsive genes, and decreased seminal vesicle weight relative to control males
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• serum LH levels are significantly increased at P180
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• testicular extracts from 180-day-old males show a~40% reduction in intratesticular testosterone levels relative to controls
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• seminal vesicle weight is significantly reduced at P75 and P180
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• although an increase in Leydig cell numbers is observed up to ~P35, the total number of Leydig cells (Cyp11a1 positive cells) per testis is significantly reduced at all ages examined
• Leydig cell hypoplasia is accompanied by reduced testicular expression of several Leydig cell markers
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• in culture, primary Leydig cells from 50-day-old males show a drastic reduction in basal testosterone as well as severely impaired testosterone synthesis in response to stimulation with hCG (human choriogonadotropin), dibutyryl(Bt2)cAMP (a permeable cAMP analog), 22OHC (22-hydroxycholesterol) or P5 (pregnenolone)
• however, cAMP accumulation in response to hCG stimulation is normal
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• when males are paired with females of known fertility, the number of vaginal plugs is significantly lower than that detected with control males
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at E17.5
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• at E17.5
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• at E16.5 and E17.5
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• at E17.5
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• at E17.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice fed a tamoxifen diet at 30 days of age show rapid deterioration of their health and die 2 weeks after starting the tamoxifen diet
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• mice fed a tamoxifen diet exhibit severe alterations in the structure of intestinal and colonic tissue
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• severe shortening of crypts in the colon of tamoxifen fed mice
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• severe shortening of crypts in the colon of tamoxifen fed mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the majority of mice die within 24 hours of birth
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• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered
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• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered
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• ear flaps are detached
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• mice are smaller at birth
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• mice lose 4%-10% of birth weight within 6 hours
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• ear flaps are detached
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• in some mice
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• mice lose 4%-10% of birth weight within 6 hours and dye is more readily absorbed than in wild-type mice
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• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered
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• ear flaps are detached
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• at E17.5, keratinocytes in the basal layer but not in the hair follicles undergo increased apoptosis compared to normal
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• mice lose 4%-10% of birth weight within 6 hours and dye is more readily absorbed than in wild-type mice
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• fewer hair follicles are present including 37% fewer peleage follicles
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• 90% fewer vibrissae follicles compared to wild-type mice
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• hair follicles undergo reduced proliferation
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• epidermis is hypoplastic
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• at E17.5, keratinocytes in the basal layer but not in the hair follicles undergo increased apoptosis compared to normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death
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• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death
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• following tamoxifen treatment, mice have increased epidermal hypoplasia and increased cell death
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• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following tamoxifen treatment, mice have increased epidermal hypoplasia and increased cell death
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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