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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Braftm1Wds
targeted mutation 1, William D Snider
MGI:3711006
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713654
cn2
 		Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1tm2Bacc 		involves: 129P2/OlaHsd * C57BL/6 MGI:3713541
cn3
 		Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1tm2Bacc
H2az2Tg(Wnt1-cre)11Rth/H2az2+ 		involves: 129P2/OlaHsd * C57BL/6J * CBA/J MGI:5659950
cn4
 		Braftm1Wds/Braftm1.1Wds
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * C57BL/6 * SJL MGI:3713535
cn5
 		Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1+
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * C57BL/6 * SJL MGI:3713536
cn6
 		Braftm1Wds/Braf+
Raf1tm2Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * C57BL/6 * SJL MGI:3713540


Genotype
MGI:3713654
cn1
Allelic
Composition		 Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (58 available)
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (114 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:121660 )
• no double conditional embryos are found live at E14-15, but can be isolated at E13

nervous system
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
abnormal innervation ( J:121660 )
• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
abnormal dorsal root ganglion morphology ( J:121660 )
• at E13, Ret levels in DRGs are reduced

cellular
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth




Genotype
MGI:3713541
cn2
Allelic
Composition		 Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1tm2Bacc
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon extension ( J:121660 )
• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons

cellular
abnormal axon extension ( J:121660 )
• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons




Genotype
MGI:5659950
cn3
Allelic
Composition		 Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1tm2Bacc
H2az2Tg(Wnt1-cre)11Rth/H2az2+
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6J * CBA/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
H2az2Tg(Wnt1-cre)11Rth mutation (2 available); any H2az2 mutation (26 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

cardiovascular system
persistent truncus arteriosus ( J:144862 )
• in 4 of 6 mice at E17.5
abnormal conotruncus morphology ( J:144862 )
• mild conotruncal defects at E16.5
ventricular septal defect ( J:144862 )
• in 4 of 6 mice at E17.5 with 1 mouse also exhibiting double outlet right ventricle

endocrine/exocrine glands
small thymus ( J:144862 )
• in 5 of 8 mice at E16.5 and E17.5
abnormal thyroid gland morphology ( J:144862 )
• hypoplastic or malpositioned in 2 of 3 mice at E16.5
small thyroid gland ( J:144862 )
• hypoplastic or malpositioned in 2 of 3 mice at E16.5

craniofacial

growth/size/body
growth/size/body region phenotype ( J:144862 )
N
• mice exhibit normal embryonic crown-rump length

hearing/vestibular/ear
hearing/vestibular/ear phenotype ( J:144862 )
N
• mice exhibit normal external ear

skeleton

immune system
small thymus ( J:144862 )
• in 5 of 8 mice at E16.5 and E17.5

hematopoietic system
small thymus ( J:144862 )
• in 5 of 8 mice at E16.5 and E17.5

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
cardiofaciocutaneous syndrome DOID:0060233 OMIM:PS115150
 J:144862




Genotype
MGI:3713535
cn4
Allelic
Composition		 Braftm1Wds/Braftm1.1Wds
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (58 available)
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality at weaning, incomplete penetrance ( J:121660 )
• mice tend to die in third week postnatal, although some survive to P35

growth/size/body
decreased body weight ( J:121660 )
• at P32, body weight is ~25% of littermates
postnatal growth retardation ( J:121660 )
• from P12-14 onwards, mice show severe growth retardation

endocrine/exocrine glands
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation to anterior lobe is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls

behavior/neurological
behavior/neurological phenotype ( J:121660 )
N
• mice show normal nociceptive response in hotplate assay
hyperactivity ( J:121660 )
• mice appear hyperactive

nervous system
nervous system phenotype ( J:121660 )
N
• dorsal root ganglion neurons survive in mutants; lumbar-vertebral neuron counts at L4 and L5 are similar to wild-type
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation to anterior lobe is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls
thin cerebral cortex ( J:121660 )
• disproportionate thinning of cortex is observed

homeostasis/metabolism
hypoglycemia ( J:121660 )
• mice display hypoglycemia with blood glucose levels ~70% below normal
abnormal body temperature homeostasis ( J:121660 )
• when separated from other mice, body temperature drops 5 degrees within 15 minutes
abnormal growth hormone level ( J:121660 )
• level is elevated in pituitary relative to controls
• level of growth hormone is elevated in individual pituitary endocrine cells




Genotype
MGI:3713536
cn5
Allelic
Composition		 Braftm1Wds/Braftm1Wds
Raf1tm2Bacc/Raf1+
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:121660 )
• most die before weaning

growth/size/body
postnatal growth retardation ( J:121660 )
• mice are smaller in the postnatal period than Braf conditional knockouts

nervous system
abnormal innervation ( J:121660 )
• deficiency of terminal axonal projections of parvalbumin-positive neurons toward the lateral motor pools of the spinal cord is seen compared to controls
abnormal sensory neuron innervation pattern ( J:121660 )
• proprioceptive axons enter spinal cord normally, but few progress beyond intermediate zone
abnormal dorsal root ganglion morphology ( J:121660 )
• at P12, number of Ret+ neurons in DRG is reduced; numbers of CGRG+ neurons are increased




Genotype
MGI:3713540
cn6
Allelic
Composition		 Braftm1Wds/Braf+
Raf1tm2Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1Wds mutation (0 available); any Braf mutation (58 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:121660 )
• mice show no phenotypic abnormalities at any stage




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory