Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
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hematopoietic system
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• irradiated wild-type mice reconstituted with Notch1/2-null bone marrow progenitors analyzed at 8 weeks show block at earliest intrathymic precursor stage; immature B cells develop in the thymus recapitulating the Notch1-null phenotype
• cultured double-null hematopoietic stem cells, HSCs do not develop into T cell progenitors after 10 days
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• cultured double null HSCs display a block at the double negative 1 stage
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immune system
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• irradiated wild-type mice reconstituted with Notch1/2-null bone marrow progenitors analyzed at 8 weeks show block at earliest intrathymic precursor stage; immature B cells develop in the thymus recapitulating the Notch1-null phenotype
• cultured double-null hematopoietic stem cells, HSCs do not develop into T cell progenitors after 10 days
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• cultured double null HSCs display a block at the double negative 1 stage
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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• at 8 weeks, dispersed gray hairs are observed
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integument
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• at 8 weeks, dispersed gray hairs are observed
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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N |
• pigmentation mediated by non-follicular melanocytes such as in the ear or eye is not affected by this mutation
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• when crossed to transgenic mice to visualize melanoblasts and melanocytes (dopachrome tautomerase - LacZ mice), at 9 days of age, a decreased number of melanocytes in the lower permanent portion of the hair follicle is observed; differences are greater at 3 and 4.5 weeks with most follicles lacking melanocytes
• mutant hair bulbs lack pigment after their first regeneration
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• coat is completetly gray with coat slightly lighter in color compared to mice missing both Notch2 alleles and one Notch1 allele
• dilution is progressive and increases with age; there is an increasing number of gray and white hairs with age such that at 7 days coat color is indistinguishable from controls, but at 7 months, coat is completely white
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integument
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• when crossed to transgenic mice to visualize melanoblasts and melanocytes (dopachrome tautomerase - LacZ mice), at 9 days of age, a decreased number of melanocytes in the lower permanent portion of the hair follicle is observed; differences are greater at 3 and 4.5 weeks with most follicles lacking melanocytes
• mutant hair bulbs lack pigment after their first regeneration
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• coat is completetly gray with coat slightly lighter in color compared to mice missing both Notch2 alleles and one Notch1 allele
• dilution is progressive and increases with age; there is an increasing number of gray and white hairs with age such that at 7 days coat color is indistinguishable from controls, but at 7 months, coat is completely white
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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• hair pigmentation is even more reduced with complete absence of Notch1 and 2 in melanocytes: hair is almost white
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integument
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• hair pigmentation is even more reduced with complete absence of Notch1 and 2 in melanocytes: hair is almost white
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Ptf1atm1(cre)Hnak mutation
(1 available);
any
Ptf1a mutation
(30 available)
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digestive/alimentary system
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• at E14.5, the acinar compartment is reduced compared to in wild-type mice
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endocrine/exocrine glands
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• at E14.5, the acinar compartment is reduced compared to in wild-type mice
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• the endocrine epithelium is mildly disrupted
• however, morphology of the islets is normal
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• the number of islets is decreased compared to in wild-type mice
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• mice exhibit a 25% decrease in the number of proliferating cells compared to in wild-type mice
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• at E13.5, pancreatic buds are less branched than in wild-type mice
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• at E13.5, pancreatic buds are smaller than controls
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• the pancreas is slightly smaller than in wild-type mice at P1
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation
(0 available);
any
Notch1 mutation
(115 available)
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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N |
• pigmentation mediated by non-follicular melanocytes such as in the ear or eye is not affected by this mutation
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• coat is completetly gray
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integument
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• coat is completetly gray
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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• at 8 weeks, dispersed gray hairs are observed
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integument
|
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• at 8 weeks, dispersed gray hairs are observed
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Tyr-cre)2Lru mutation
(0 available)
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pigmentation
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N |
• loss of one allele has no affect on coat color; no graying or dilution is observed in 8-week old mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation
(0 available);
any
Notch2 mutation
(97 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
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hematopoietic system
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N |
• no ectopic B cell development in the thymus is observed after injection of mutant bone marrow progenitors into irradiated wild-type hosts
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• hematopoietic stem cells do not develop in to B cells after 18 days in culture
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• loss of MZ B cells in spleen is detected
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immune system
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• hematopoietic stem cells do not develop in to B cells after 18 days in culture
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• loss of MZ B cells in spleen is detected
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Analysis Tools