Phenotypes associated with this allele

• Allele Symbol: Chat^tm1(cre)Lowl
• Allele Name: targeted mutation 1, Bradford B Lowell
• Allele ID: MGI:3689420
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Chat^tm1(cre)Lowl/Chat^+	involves: 129S6/SvEvTac * C57BL/6	MGI:3689725
cn2	Brsk2^tm2.1Jrs/Brsk2^tm2.1Jrs Chat^tm1(cre)Lowl/Chat^+	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:5524149
cn3	Mc4r^tm1Lowl/Mc4r^tm1Lowl Chat^tm1(cre)Lowl/Chat^+	involves: 129S4/SvJae * 129S6/SvEvTac	MGI:5484740
cn4	Chat^tm1(cre)Lowl/Chat^+ Fus^tm1Ics/Fus^+	involves: 129S6/SvEvTac * C57BL/6	MGI:8435668
cn5	Mc4r^tm2Lowl/Mc4r^tm2Lowl Chat^tm1(cre)Lowl/Chat^+ Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor^+	involves: 129S6/SvEvTac * C57BL/6NCr	MGI:5484737

Genotype
MGI:3689725

ht1

• Allelic Composition: Chat^tm1(cre)Lowl/Chat⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:114556 )

• heterozygotes are viable and fertile; cre recombinase activity is detected in all cholinergic neurons

Genotype
MGI:5524149

cn2

• Allelic Composition: Brsk2^tm2.1Jrs/Brsk2^tm2.1Jrs; Chat^tm1(cre)Lowl/Chat⁺
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system

nervous system phenotype ( J:201695 )

N • type Ia proprioceptive sensory neuron projections grow normally to the ventral horn

Genotype
MGI:5484740

cn3

• Allelic Composition: Mc4r^tm1Lowl/Mc4r^tm1Lowl; Chat^tm1(cre)Lowl/Chat⁺
• Genetic Background: involves: 129S4/SvJae * 129S6/SvEvTac

cardiovascular system

hypertension ( J:193452 )

• develop obesity induced hypertension but only during active period

homeostasis/metabolism

increased circulating insulin level ( J:193452 )

• higher plasma insulin levels

Genotype
MGI:8435668

cn4

• Allelic Composition: Chat^tm1(cre)Lowl/Chat⁺; Fus^tm1Ics/Fus⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:371167 )

• at 22 months of age, but not 10 months of age, mice show impaired motor performance, indicating delayed motor deficits

cellular

increased oligodendrocyte number ( J:371167 )

• oligodendrocytes are more numerous in ventral horn white matter in 22-month-old mice

muscle

muscle weakness ( J:371167 )

• 22-month-old mice show shorter hanging time and decreased holding impulse (represents the total sustained force exerted by the mouse to oppose the gravitational force) in an inverted grid test

nervous system

nervous system phenotype ( J:371167 )

N • mice show prevention of motor neuron degeneration, with normal motor neuron numbers at 22 months of age and do not develop electromyography abnormalities

increased oligodendrocyte number ( J:371167 )

• oligodendrocytes are more numerous in ventral horn white matter in 22-month-old mice

abnormal motor neuron morphology ( J:371167 )

• motor neuron axons show a distribution shifted towards smaller caliber in 22-month-old mice

Genotype
MGI:5484737

cn5

• Allelic Composition: Mc4r^tm2Lowl/Mc4r^tm2Lowl; Chat^tm1(cre)Lowl/Chat⁺; Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCr

nervous system

nervous system phenotype ( J:193452 )

N • biophysical properties of cholinergic neurons are normal

abnormal nervous system electrophysiology ( J:193452 )

• melanotan II fails to hyperpolarize membrane potentials of all dorsal motor nucleus vagal cholinergic neurons

• similar results with Mc4r agonist THIQ