Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^tm1Fia
• Allele Name: targeted mutation 1, Fraz A Ismat
• Allele ID: MGI:3689410
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor^+ Nf1^tm1Par/Nf1^tm1Par Tg(Fabp7-cre,-lacZ)3Gtm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:3810651
cn2	Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor^+ Nf1^tm1Fcr/Nf1^tm1Par Tg(Tek-cre)1Ywa/0	involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3689708
cn3	Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor^+ Hprt1^tm1(CMV-cre)Brd/Hprt1^+ Nf1^tm1Fcr/Nf1^tm1Fcr	involves: 129S/SvEv * 129S/SvEvBrd	MGI:3689632
cn4	Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor^tm1Fia Hprt1^tm1(CMV-cre)Brd/Hprt1^+ Nf1^tm1Fcr/Nf1^tm1Fcr	involves: 129S/SvEv * 129S/SvEvBrd	MGI:3689697

Genotype
MGI:3810651

cn1

• Allelic Composition: Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor⁺; Nf1^tm1Par/Nf1^tm1Par; Tg(Fabp7-cre,-lacZ)3Gtm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

small adenohypophysis ( J:138868 )

• significantly smaller than control littermates

growth/size/body

decreased body weight ( J:138868 )

endocrine/exocrine glands

small adenohypophysis ( J:138868 )

• significantly smaller than control littermates

homeostasis/metabolism

decreased circulating growth hormone level ( J:138868 )

• reduction in liver Igf1 mRNA levels indicates a reduction in circulating growth hormone levels

Genotype
MGI:3689708

cn2

• Allelic Composition: Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor⁺; Nf1^tm1Fcr/Nf1^tm1Par; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

mortality/aging

prenatal lethality, complete penetrance ( J:114455 )

• 2 embryos are found at later stages, after expected midgestation lethality from cardiovascular failure

hematopoietic system

abnormal spleen morphology ( J:114455 )

• mice display moderate splenic enlargement and partial rescue of splenic architecture

• germinal centers are present

• splenic fibrosis is not observed

immune system

abnormal spleen morphology ( J:114455 )

• mice display moderate splenic enlargement and partial rescue of splenic architecture

• germinal centers are present

• splenic fibrosis is not observed

nervous system

abnormal dorsal root ganglion morphology ( J:114455 )

• marked enlargement of dorsal root ganglia and other neural crest derived tissues is observed

Genotype
MGI:3689632

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor⁺; Hprt1^{tm1(CMV-cre)Brd}/Hprt1⁺; Nf1^tm1Fcr/Nf1^tm1Fcr
• Genetic Background: involves: 129S/SvEv * 129S/SvEvBrd

mortality/aging

prenatal lethality, incomplete penetrance ( J:114455 )

♀ • some mice (5/208) survive to birth while all Nf1-null homozygotes expressing either Gt(ROSA)26Sor^tm1Fia or Hprt1^{tm1(CMV-cre)Brd} die prior to birth

nervous system

abnormal sympathetic ganglion morphology ( J:114455 )

♀ • massively enlarged

abnormal somatic sensory system morphology ( J:114455 )

♀ • peripheral ganglia are massively enlarged in newborns

abnormal dorsal root ganglion morphology ( J:114455 )

♀ • enormous paraspinal masses arise from the dorsal root ganglia

endocrine/exocrine glands

adrenal medulla hyperplasia ( J:114455 )

♀ • medulla is overgrown compared with wild-type, with cortical effacement and tumor-like medullary protrusion

homeostasis/metabolism

abnormal enzyme/coenzyme level ( J:114455 )

♀ • elevate Ras levels return to wild-type levels by E12.5 with expression of Gt(ROSA)26Sor^tm1Fia

Genotype
MGI:3689697

cn4

• Allelic Composition: Gt(ROSA)26Sor^tm1Fia/Gt(ROSA)26Sor^tm1Fia; Hprt1^{tm1(CMV-cre)Brd}/Hprt1⁺; Nf1^tm1Fcr/Nf1^tm1Fcr
• Genetic Background: involves: 129S/SvEv * 129S/SvEvBrd

nervous system

abnormal somatic sensory system morphology ( J:114455 )

♀ • peripheral ganglia are massively enlarged in newborns