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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Braftm1.1Sva
targeted mutation 1.1, Alcino Silva
MGI:3622462
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Braftm1.1Sva/Braftm1.1Sva involves: 129S1/Sv * 129T2/SvEms * 129X1/SvJ MGI:3622481
hm2
 		Braftm1.1Sva/Braftm1.1Sva involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3711452
cn3
 		Braftm1Sva/Braftm1.1Sva
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713581
cn4
 		Braftm1Sva/Braftm1.1Sva
Meox2tm1(cre)Sor/Meox2+ 		involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ MGI:3622484
cn5
 		Braftm1Sva/Braftm1.1Sva
Tg(Nes-cre)1Kln/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713552


Genotype
MGI:3622481
hm1
Allelic
Composition		 Braftm1.1Sva/Braftm1.1Sva
Genetic
Background		 involves: 129S1/Sv * 129T2/SvEms * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (58 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Embryonic development abnormalities in Braftm1.1Sva/Braftm1.1Sva mice

mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:105998 )
• no viable homozygotes after E11.5
• embryos at E11.5 display extensive apoptosis in the liver, brain, and heart

growth/size/body
decreased embryo size ( J:105998 )
• embryos are significantly smaller than controls at E11.5

embryo
decreased embryo size ( J:105998 )
• embryos are significantly smaller than controls at E11.5
abnormal placenta morphology ( J:105998 )
• fetal part of placenta is thinner and less vascularized
abnormal placenta labyrinth morphology ( J:105998 )
• severely underdeveloped
abnormal placental labyrinth vasculature morphology ( J:105998 )
• few patent fetal blood vessels which fail to make proper contact with maternal blood sinuses
abnormal trophoblast layer morphology ( J:105998 )
• discontinuous giant trophoblast layer
abnormal spongiotrophoblast layer morphology ( J:105998 )
• discontinuous spongiotrophoblast layer

cardiovascular system
abnormal placental labyrinth vasculature morphology ( J:105998 )
• few patent fetal blood vessels which fail to make proper contact with maternal blood sinuses




Genotype
MGI:3711452
hm2
Allelic
Composition		 Braftm1.1Sva/Braftm1.1Sva
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (58 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

embryo
embryonic growth retardation ( J:121660 )
• embryos show obvious growth retardation prior to death

growth/size/body
embryonic growth retardation ( J:121660 )
• embryos show obvious growth retardation prior to death

nervous system
increased neuron apoptosis ( J:121660 )
• ~90% of cultured DRG neurons from E12.5 embryos die within 5 days in presence of nerve growth factor compared to 19% loss of heterozygous control neurons

cellular
increased neuron apoptosis ( J:121660 )
• ~90% of cultured DRG neurons from E12.5 embryos die within 5 days in presence of nerve growth factor compared to 19% loss of heterozygous control neurons




Genotype
MGI:3713581
cn3
Allelic
Composition		 Braftm1Sva/Braftm1.1Sva
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (58 available)
Braftm1Sva mutation (1 available); any Braf mutation (58 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (114 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (114 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:121660 )
• no double conditional embryos are found live at E14-15, but can be isolated at E13

nervous system
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
abnormal innervation ( J:121660 )
• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
abnormal dorsal root ganglion morphology ( J:121660 )
• at E13, Ret levels in DRGs are reduced

cellular
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth




Genotype
MGI:3622484
cn4
Allelic
Composition		 Braftm1Sva/Braftm1.1Sva
Meox2tm1(cre)Sor/Meox2+
Genetic
Background		 involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (58 available)
Braftm1Sva mutation (1 available); any Braf mutation (58 available)
Meox2tm1(cre)Sor mutation (3 available); any Meox2 mutation (18 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality at weaning, complete penetrance ( J:105998 )
• although born alive death occurs around 21 days of age from aggressive neurodegenerative disease

growth/size/body
postnatal growth retardation ( J:105998 )
• after being born alive, pups show progressive growth retardation




Genotype
MGI:3713552
cn5
Allelic
Composition		 Braftm1Sva/Braftm1.1Sva
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (58 available)
Braftm1Sva mutation (1 available); any Braf mutation (58 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality at weaning, incomplete penetrance ( J:121660 )
• mice tend to die in third week postnatal, although some survive to P35

growth/size/body
decreased body weight ( J:121660 )
• at P32, body weight is ~25% of littermates
postnatal growth retardation ( J:121660 )
• from P12-14 onwards, mice show severe growth retardation

behavior/neurological
behavior/neurological phenotype ( J:121660 )
N
• mice show normal nociceptive response in hotplate assay
hyperactivity ( J:121660 )
• mice appear hyperactive

nervous system
nervous system phenotype ( J:121660 )
N
• dorsal root ganglion neurons survive in mutants
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls
thin cerebral cortex ( J:121660 )
• disproportionate thinning of cortex is observed

endocrine/exocrine glands
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls

homeostasis/metabolism
hypoglycemia ( J:121660 )
• mice display significantly reduced bolood glucose levels
abnormal body temperature homeostasis ( J:121660 )
• when separated from other mice, body temperature drops rapidly
abnormal growth hormone level ( J:121660 )
• level is elevated in pituitary relative to controls
• level of growth hormone is elevated in individual pituitary endocrine cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory