Phenotypes associated with this allele
Allelic
Composition |
Rasgrf1tm4.1Pds/Rasgrf1+
|
|
Genetic
Background |
involves: 129S4/SvJaeSor * C57BL/6 |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrf1tm4.1Pds mutation
(0 available);
any
Rasgrf1 mutation
(65 available)
|
|
|
normal phenotype
|
|
• DNA methylation of and Rasgrf1 expression from floxed allele are unaffected
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Meox2tm1(cre)Sor mutation
(3 available);
any
Meox2 mutation
(18 available)
Rasgrf1tm4.1Pds mutation
(0 available);
any
Rasgrf1 mutation
(65 available)
|
|
|
cellular
|
N |
• loss of paternal repeats does not affect expression of Rasgrf1 in neonatal brain
• loss of paternal repeats at E5.5 in the epiblast does not result in changes to methylation state of the paternal allele, in contrast with deletion earlier in development
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrf1tm4.1Pds mutation
(0 available);
any
Rasgrf1 mutation
(65 available)
Tg(Zp3-cre)3Mrt mutation
(2 available)
|
|
|
cellular
|
|
• Rasgrf1 expression from paternal allele is silenced in neonatal brain
|
|
|
• DNA from progeny has lost almost all differentially methylated domain (DMD) methylation from the paternal allele
• methylation on floxed allele cannot be maintained upon cre-mediated recombination in zygote
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rasgrf1tm4.1Pds mutation
(0 available);
any
Rasgrf1 mutation
(65 available)
Tg(Nes-cre)1Kln mutation
(4 available)
|
|
|
cellular
|
N |
• loss of paternal repeats does not affect expression of Rasgrf1 in neonatal brain
• loss of paternal repeats at E11 in the central nervous system does not result in changes to methylation state of the paternal allele, in contrast with deletion earlier in development
|
Analysis Tools
