Phenotypes associated with this allele

• Allele Symbol: Tg(Upk2-HRAS*Q61L)5Xrw
• Allele Name: transgene insertion 5, Xue-ru Wu
• Allele ID: MGI:3522719
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt Tg(Upk2-cre)6Xrw/0 Tg(Upk2-HRAS*Q61L)5Xrw/0	involves: 129X1/SvJ * FVB/N	MGI:5790497
tg2	Tg(Upk2-HRAS*Q61L)5Xrw/?	involves: FVB/N	MGI:5789930

Genotype
MGI:5790497

cn1

• Allelic Composition: Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt; Tg(Upk2-cre)6Xrw/0; Tg(Upk2-HRAS*Q61L)5Xrw/0
• Genetic Background: involves: 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:234236 )

• mean survival of 231 days and median survival of 236 days

neoplasm

increased urinary bladder carcinoma incidence ( J:234236 )

• mice rapidly develop bladder tumors with progression to non-invasive papillary carcinomas (urothelial cell carcinoma)

• the number of proliferating cells within each tumor is increased compared to single conditional Ctnnb1^tm1Mmt homozygotes

• mice treated with a MEK1/2 inhibitor, PD184352, show tumor regression and reduction in proliferation

renal/urinary system

hematuria ( J:234236 )

• due to bladder tumors

increased urinary bladder carcinoma incidence ( J:234236 )

• mice rapidly develop bladder tumors with progression to non-invasive papillary carcinomas (urothelial cell carcinoma)

• the number of proliferating cells within each tumor is increased compared to single conditional Ctnnb1^tm1Mmt homozygotes

• mice treated with a MEK1/2 inhibitor, PD184352, show tumor regression and reduction in proliferation

homeostasis/metabolism

hematuria ( J:234236 )

• due to bladder tumors

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
urinary bladder cancer		DOID:11054	OMIM:109800	J:234236

Genotype
MGI:5789930

tg2

• Allelic Composition: Tg(Upk2-HRAS*Q61L)5Xrw/?
• Genetic Background: involves: FVB/N

neoplasm

increased urinary system tumor incidence ( J:69251 )

• 63% of mice develop superficial papillary bladder tumors from 10 to 26 months of age

renal/urinary system

increased urinary system tumor incidence ( J:69251 )

• 63% of mice develop superficial papillary bladder tumors from 10 to 26 months of age

abnormal urinary bladder urothelium morphology ( J:69251 , J:234236 )

• at 3-5 months of age, the urothelium becomes thickened to 6-7 cell layers, indicating urothelial hyperplasia (J:69251)

• urothelium becomes even more hyperplastic by 8 months of age, forming localized epithelial nodules (J:69251)

• in mice that develop bladder papillary tumors, the intervening urothelium shows simple or nodular hyperplasia and mice that dont develop tumors exhibit simple and nodular urothelial hyperplasia (J:69251)

• hyperplasia of the urothelium is seen as early as 3 months of age with increasing thickness at 12 months (J:234236)