Phenotypes associated with this allele

• Allele Symbol: Ptk2^tm1Gwm
• Allele Name: targeted mutation 1, Gordon W McLean
• Allele ID: MGI:3521998
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ptk2^tm1Gwm/Ptk2^tm1Gwm Tg(KRT14-cre/ERT2)1Ipc/0	involves: 129P2/OlaHsd * FVB	MGI:3527814

Genotype
MGI:3527814

cn1

• Allelic Composition: Ptk2^tm1Gwm/Ptk2^tm1Gwm; Tg(KRT14-cre/ERT2)1Ipc/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

decreased incidence of tumors by chemical induction ( J:94680 )

• only 50% of tamoxifen treated mice subjected to carcinogens DMBA and TPA formed papillomas after 12 weeks compared to 100% of untreated mice

• mice treated with DMBA and TPA and subsequently treated with tamoxifen after majority of papillomas had formed, showed a reduction in the malignant conversion of papillomas into squamous cell carcinoma (0.6% of papillomas formed carcinoma compared to 4.1% in controls and the 0.6% did not have Ptk2 excised by tamoxifen treatment)

decreased tumor growth/size ( J:94680 )

• substantial reduction in the average number of papillomas formed per tamoxifen treated mouse at 22 weeks when compared to untreated controls

integument

increased keratinocyte apoptosis ( J:94680 )

• loss of normal cell cycle profiles and a substantial amount of cell death in tamoxifen treated primary keratinocytes

decreased keratinocyte migration ( J:94680 )

• tamoxifen treated primary keratinocytes were unable to repopulate denuded areas of wounded monolayers and displayed ~50% reduced migration rates, however saw no visible difference in punch biopsy would repair assays

homeostasis/metabolism

decreased incidence of tumors by chemical induction ( J:94680 )

• only 50% of tamoxifen treated mice subjected to carcinogens DMBA and TPA formed papillomas after 12 weeks compared to 100% of untreated mice

• mice treated with DMBA and TPA and subsequently treated with tamoxifen after majority of papillomas had formed, showed a reduction in the malignant conversion of papillomas into squamous cell carcinoma (0.6% of papillomas formed carcinoma compared to 4.1% in controls and the 0.6% did not have Ptk2 excised by tamoxifen treatment)

cellular

increased keratinocyte apoptosis ( J:94680 )

• loss of normal cell cycle profiles and a substantial amount of cell death in tamoxifen treated primary keratinocytes

decreased keratinocyte migration ( J:94680 )

• tamoxifen treated primary keratinocytes were unable to repopulate denuded areas of wounded monolayers and displayed ~50% reduced migration rates, however saw no visible difference in punch biopsy would repair assays