Ptk2^tm1Gwm
Targeted Allele Detail

Summary

• Symbol: Ptk2^tm1Gwm
• Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Gordon W McLean
• MGI ID: MGI:3521998
• Synonyms: FAK^flox
• Gene: Ptk2 Location: Chr15:73076951-73295129 bp, - strand Genetic Position: Chr15, 33.94 cM
• Alliance: Ptk2^tm1Gwm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94680
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to introduce loxP sites to flank the exon encoding part of the kinase domain, encoded by amino acids 413-444. Upon transfection with a Cre-expressing plasmid, the floxed PGK-neo cassette was deleted, leaving the exon floxed. The targeting vector was designed so that Cre-mediated recombination would introduce a frameshift was created in the adjacent exon, preventing production of a functional protein. Immunoblot confirmed the lack of wild-type protein or truncated protein created by this allele in Cre-expressing ES cells. (J:94680)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptk2 Mutation: 91 strains or lines available

References

• Original: J:94680 McLean GW, et al., Specific deletion of focal adhesion kinase suppresses tumor formation and blocks malignant progression. Genes Dev. 2004 Dec 15;18(24):2998-3003
• All: 23 reference(s)