Phenotypes associated with this allele

• Allele Symbol: Pax8^{tm1.1(cre)Mbu}
• Allele Name: targeted mutation 1.1, Meinrad Busslinger
• Allele ID: MGI:3050216
• Summary: 10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pax8^tm1Rdl/Pax8^tm1.1(cre)Mbu	B6.129(Cg)-Pax8^tm1Rdl Pax8^tm1.1(cre)Mbu	MGI:5775543
cn2	Pax8^tm1Rdl/Pax8^tm1.1(cre)Mbu	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5299328
cn3	Dicer1^tm1Smr/Dicer1^tm1Smr Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * 129S7/SvEvBrd	MGI:5792829
cn4	Pax8^tm1.1(cre)Mbu/Pax8^+ Plxnb2^tm1.1Rkun/Plxnb2^tm1.1Rkun	involves: 129P2/OlaHsd * C57BL/6	MGI:5807121
cn5	Pax8^tm1.1(cre)Mbu/Pax8^+ Plxnb1^tm1Rkun/Plxnb1^tm1Rkun Plxnb2^tm1.1Rkun/Plxnb2^tm1.1Rkun	involves: 129P2/OlaHsd * C57BL/6	MGI:5807122
cn6	Hnrnpf^tm1Jsdc/Hnrnpf^tm1Jsdc Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * C57BL/6	MGI:6392034
cn7	Gal3st1^tm1.1Hjg/Gal3st1^tm1.1Hjg Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5521179
cn8	Gal3st1^tm1.1Hjg/Gal3st1^tm1.1Hjg Ugcg^tm1Hjg/Ugcg^tm1Hjg Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5521180
cn9	Ugcg^tm1Hjg/Ugcg^tm1Hjg Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5521181
cn10	Dkk1^tm1.1Svo/Dkk1^tm1.2Svo Pax8^tm1.1(cre)Mbu/Pax8^+	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5013426

Genotype
MGI:5775543

cn1

• Allelic Composition: Pax8^tm1Rdl/Pax8^{tm1.1(cre)Mbu}
• Genetic Background: B6.129(Cg)-Pax8^tm1Rdl Pax8^{tm1.1(cre)Mbu}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:220108 )

• all mice die within 5 weeks of birth

growth/size/body

delayed ear emergence ( J:220108 )

• mice exhibit a delay in ear extension relative to controls

decreased body size ( J:220108 )

• at 1 month of age, mice are smaller than control littermates

decreased body weight ( J:220108 )

• at 1 month of age

vision/eye

delayed eyelid opening ( J:220108 )

• mice exhibit a delay in eyelid opening relative to controls

endocrine/exocrine glands

abnormal thyroid gland morphology ( J:220108 )

• at 1 month of age, the thyroid gland appears as a cellular mass almost devoid of follicles

abnormal thyroid follicle morphology ( J:220108 )

• at 1 month of age, the thyroid gland does not display the follicular structure typical of normal glands

abnormal thyroid follicular cell morphology ( J:220108 )

• at P21, many de-differentiatedthyroid follicular cells are present, unlike in control glands

small thyroid gland ( J:220108 )

• at 1 month of age, the thyroid gland is considerably smaller than in controls

decreased activity of thyroid gland ( J:220108 )

• at 1 month of age, mice exhibit hypothyroidism associated with reduced thyroid function

homeostasis/metabolism

increased circulating thyroid-stimulating hormone level ( J:220108 )

• at 1 month of age, mean serum TSH levels are increased by 4-fold relative to controls

decreased circulating thyroxine level ( J:220108 )

• at 1 month of age, serum T4 levels are almost undetectable

cellular

increased apoptosis ( J:220108 )

• at P1 and P21, mice exhibit scattered caspase-3 positive cells in the thyroid gland, unlike control littermates

craniofacial

delayed ear emergence ( J:220108 )

• mice exhibit a delay in ear extension relative to controls

hearing/vestibular/ear

delayed ear emergence ( J:220108 )

• mice exhibit a delay in ear extension relative to controls

Genotype
MGI:5299328

cn2

• Allelic Composition: Pax8^tm1Rdl/Pax8^{tm1.1(cre)Mbu}
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

cellular

increased apoptosis ( J:178498 )

• in the thyroid gland

Genotype
MGI:5792829

cn3

• Allelic Composition: Dicer1^tm1Smr/Dicer1^tm1Smr; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd

renal/urinary system

increased kidney apoptosis ( J:229188 )

• apoptosis is frequently seen in shedding cells in both the Bowman space and in the lumen of the tubules, occurring more frequently at P50

increased kidney cell proliferation ( J:229188 )

• the tubular epithelium and parietal cells of the renal corpuscles show increased cellular proliferation in the phase of cyst growth

decreased urine osmolality ( J:229188 )

• at P30, urinary osmolality is lower, with no changes in urinary ouput or in collecting duct distribution

increased urine protein level ( J:229188 )

• mice develop progressively massive proteinuria, consisting mainly of albuminuria that is already higher at P30

albuminuria ( J:229188 )

• seen by P30

abnormal kidney morphology ( J:229188 )

• kidneys at P50 have a rough surface

abnormal renal tubule epithelial cell primary cilium morphology ( J:229188 )

• at P50, primary cilia are rare in the epithelium of dilating tubules and absent in enlarged cysts with flattened epithelium, indicating a progressive loss of primary cilia with cyst enlargement

kidney cortex cyst ( J:229188 )

• at P50, mice exhibit cortical cysts

• cysts originate from the renal corpuscles of the cortex and are absent in the outer and inner medulla

decreased kidney collecting duct number ( J:229188 )

• the density of collecting ducts is lower at P50

abnormal renal corpuscle morphology ( J:229188 )

• the tubular epithelium and parietal cells of the renal corpuscles show increased cellular proliferation in the phase of cyst growth, before flattening of the cystic epithelium

dilated renal glomerular capsule ( J:229188 )

• dilatation of the parietal cells of the Bowman capsule

abnormal renal glomerulus morphology ( J:229188 )

• glomeruli progressively shrink

• mice develop glomerulocystic disease

renal interstitial fibrosis ( J:229188 )

• epithelium lining the cyst flattens and interstitial fibrosis develops

decreased kidney weight ( J:229188 )

• mice present lower kidney weight at P50, with the ratio of kidney weight/body weight being lower at P50 but not P30

dilated renal tubule ( J:229188 )

• tubular dilatations are mainly cortical and develop progressively at P50

pale kidney ( J:229188 )

• kidneys at P50 have a paler color

decreased renal glomerular filtration rate ( J:229188 )

isosthenuria ( J:229188 )

• progressive impairment of urinary concentrating ability

polyuria ( J:229188 )

• increase in urine output at P50 but not P30

cellular

abnormal renal tubule epithelial cell primary cilium morphology ( J:229188 )

• at P50, primary cilia are rare in the epithelium of dilating tubules and absent in enlarged cysts with flattened epithelium, indicating a progressive loss of primary cilia with cyst enlargement

increased kidney apoptosis ( J:229188 )

• apoptosis is frequently seen in shedding cells in both the Bowman space and in the lumen of the tubules, occurring more frequently at P50

increased kidney cell proliferation ( J:229188 )

• the tubular epithelium and parietal cells of the renal corpuscles show increased cellular proliferation in the phase of cyst growth

growth/size/body

decreased body weight ( J:229188 )

• mice present lower body weight at P30

kidney cortex cyst ( J:229188 )

• at P50, mice exhibit cortical cysts

• cysts originate from the renal corpuscles of the cortex and are absent in the outer and inner medulla

homeostasis/metabolism

decreased urine osmolality ( J:229188 )

• at P30, urinary osmolality is lower, with no changes in urinary ouput or in collecting duct distribution

increased urine protein level ( J:229188 )

• mice develop progressively massive proteinuria, consisting mainly of albuminuria that is already higher at P30

albuminuria ( J:229188 )

• seen by P30

behavior/neurological

increased fluid intake ( J:229188 )

• increase in water intake at P30 and P50

Genotype
MGI:5807121

cn4

• Allelic Composition: Pax8^{tm1.1(cre)Mbu}/Pax8⁺; Plxnb2^tm1.1Rkun/Plxnb2^tm1.1Rkun
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

homeostasis/metabolism

increased susceptibility to kidney reperfusion injury ( J:221423 )

• mice show no signs of recovery from induced bilateral kidney injury and become moribund 7 days after injury

• all tubules of the entire renal cortex and outer medulla show abnormal epithelial architecture after injury

renal/urinary system

increased susceptibility to kidney reperfusion injury ( J:221423 )

• mice show no signs of recovery from induced bilateral kidney injury and become moribund 7 days after injury

• all tubules of the entire renal cortex and outer medulla show abnormal epithelial architecture after injury

abnormal renal tubule epithelium morphology ( J:221423 )

• kidney histology during the acute organ damage 12 and 24 hours after ischemia/reperfusion injury is similar to wild-type mice, however 3 days after injury, renal tubular epithelial cells leave the epithelial plane and intrude into the tubular lumen which leads to a multi-layered epithelium and complete tubular occlusion by day 7

• renal tubular epithelial cells one day after injury exhibit a mitotic spindle angle that is shifted toward divisions perpendicular to the epithelial plane

• 3 days after kidney injury, a proportion of renal tubular epithelial cells fail to align their divisions with the epithelial plane

• however, kidney morphology and function are normal under basal conditions

Genotype
MGI:5807122

cn5

• Allelic Composition: Pax8^{tm1.1(cre)Mbu}/Pax8⁺; Plxnb1^tm1Rkun/Plxnb1^tm1Rkun; Plxnb2^tm1.1Rkun/Plxnb2^tm1.1Rkun
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

renal/urinary system

abnormal renal tubule epithelium morphology ( J:221423 )

• mice exhibit foci of multi-layered tubules

Genotype
MGI:6392034

cn6

• Allelic Composition: Hnrnpf^tm1Jsdc/Hnrnpf^tm1Jsdc; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cardiovascular system

hypertension ( J:284239 )

• mice develop hypertension by 8 weeks of age

increased systemic arterial systolic blood pressure ( J:284239 )

• mice exhibit higher systolic blood pressure from 6 to 24 weeks of age

homeostasis/metabolism

increased angiotensin II level ( J:284239 )

• increase in urinary angiotensin II levels at 24 weeks of age

abnormal urine homeostasis ( J:284239 )

• increase in urinary angiotensin II levels at 24 weeks of age

increased urine glucose level ( J:284239 )

• increase in glucose excretion in the urine at 6 weeks of age, indicating development of glycosuria

• from 8 weeks of age, urinary glucose levels in males decrease and return to levels similar to wild-type mice at 12 weeks of age

• urinary glucose excretion in females steadily increases from week 6, reaches a plateau at 12 weeks of age and does not abate

• however, mice exhibit normal non-fasting blood glucose levels and normal glucose tolerance at 23 weeks of age

albuminuria ( J:284239 )

• increase in urinary albumin/creatinine ratio at 24 weeks of age

renal/urinary system

abnormal urine homeostasis ( J:284239 )

• increase in urinary angiotensin II levels at 24 weeks of age

increased urine glucose level ( J:284239 )

• increase in glucose excretion in the urine at 6 weeks of age, indicating development of glycosuria

• from 8 weeks of age, urinary glucose levels in males decrease and return to levels similar to wild-type mice at 12 weeks of age

• urinary glucose excretion in females steadily increases from week 6, reaches a plateau at 12 weeks of age and does not abate

• however, mice exhibit normal non-fasting blood glucose levels and normal glucose tolerance at 23 weeks of age

albuminuria ( J:284239 )

• increase in urinary albumin/creatinine ratio at 24 weeks of age

renal interstitial fibrosis ( J:284239 )

• tubule-interstitial fibrosis is seen in the kidneys at 24 weeks of age

abnormal renal tubule morphology ( J:284239 )

• increase of tubular luminal area

• however, mice show no obvious structural changes in the kidneys at 24 weeks of age, normal kidney weight/body weight ratio, normal glomerular filtration rate, and normal glomerular tuft volume

abnormal proximal convoluted tubule morphology ( J:284239 )

• increase of renal proximal tubular cell volume

polyuria ( J:284239 )

• 24 hour urine volume is increased

• however, food and water intake are normal and no differences in serum and urine levels of sodium, calcium, and phosphorus are seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
renal glycosuria		DOID:9432	OMIM:233100	J:284239

Genotype
MGI:5521179

cn7

• Allelic Composition: Gal3st1^tm1.1Hjg/Gal3st1^tm1.1Hjg; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

mortality/aging ( J:197393 )

N • normal life span

renal/urinary system

renal/urinary system phenotype ( J:197393 )

N • normal kidney morphology and creatinine clearance

abnormal urine homeostasis ( J:197393 )

decreased urine ammonia level ( J:197393 )

• decreased ammonium excretion

• reduced ability to increase urinary ammonia

increased urine potassium level ( J:197393 )

decreased urine pH ( J:197393 )

• higher excretion of titratable acid relative to controls

• less ability to recover from high acid in the diet

polyuria ( J:197393 )

homeostasis/metabolism

metabolic acidosis ( J:197393 )

• increased metabolic acidosis

abnormal urine homeostasis ( J:197393 )

decreased urine ammonia level ( J:197393 )

• decreased ammonium excretion

• reduced ability to increase urinary ammonia

increased urine potassium level ( J:197393 )

decreased urine pH ( J:197393 )

• higher excretion of titratable acid relative to controls

• less ability to recover from high acid in the diet

Genotype
MGI:5521180

cn8

• Allelic Composition: Gal3st1^tm1.1Hjg/Gal3st1^tm1.1Hjg; Ugcg^tm1Hjg/Ugcg^tm1Hjg; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

mortality/aging ( J:197393 )

N • normal life span

renal/urinary system

renal/urinary system phenotype ( J:197393 )

N • normal kidney morphology and creatinine clearance

abnormal urine homeostasis ( J:197393 )

decreased urine ammonia level ( J:197393 )

• decreased ammonium excretion

• reduced ability to increase urinary ammonia

increased urine potassium level ( J:197393 )

decreased urine pH ( J:197393 )

• higher excretion of titratable acid relative to controls

• less ability to recover from high acid in the diet

abnormal kidney papilla morphology ( J:197393 )

• ammonium content of the renal papilla is reduced 30% compared to controls

polyuria ( J:197393 )

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:197393 )

N • normal blood pH

N • normal blood bicarbonate levels

abnormal urine homeostasis ( J:197393 )

decreased urine ammonia level ( J:197393 )

• decreased ammonium excretion

• reduced ability to increase urinary ammonia

increased urine potassium level ( J:197393 )

decreased urine pH ( J:197393 )

• higher excretion of titratable acid relative to controls

• less ability to recover from high acid in the diet

Genotype
MGI:5521181

cn9

• Allelic Composition: Ugcg^tm1Hjg/Ugcg^tm1Hjg; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

mortality/aging ( J:197393 )

N • normal life span

renal/urinary system

renal/urinary system phenotype ( J:197393 )

N • normal kidney morphology and creatinine clearance

decreased urine pH ( J:197393 )

homeostasis/metabolism

decreased urine pH ( J:197393 )

Genotype
MGI:5013426

cn10

• Allelic Composition: Dkk1^tm1.1Svo/Dkk1^tm1.2Svo; Pax8^{tm1.1(cre)Mbu}/Pax8⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

renal/urinary system

increased kidney cell proliferation ( J:173125 )

• mice exhibit increased proliferation of epithelial tubules of the developing kidney papilla compared with wild-type mice

decreased urine creatinine level ( J:173125 )

decreased urine glucose level ( J:173125 )

increased urine chloride ion level ( J:173125 )

• mice exhibit increased chloride in the urine compared with wild-type mice

decreased urine potassium level ( J:173125 )

decreased urine sodium level ( J:173125 )

increased urine calcium level ( J:173125 )

decreased urine phosphate level ( J:173125 )

decreased urine protein level ( J:173125 )

abnormal kidney collecting duct epithelium morphology ( J:173125 )

• mice exhibit hypertrophic collecting duct epithelial cells at the tip of the extended renal papilla compared with wild-type mice

abnormal kidney medulla morphology ( J:173125 )

elongated kidney papilla ( J:173125 )

• mice exhibit overgrowth of renal papilla in proportion to the rest of the kidney unlike in wild-type mice

• the papilla in the medulla extends over the lateral edge of the kidney capsule and reached the epithelial lining of the lumen of the ureter outside the kidney unlike in wild-type mice

• 7 of 10 mice exhibit over extended papilla unlike in wild-type mice

hydronephrosis ( J:173125 )

• in 2 of 10 mice

oliguria ( J:173125 )

homeostasis/metabolism

decreased urine creatinine level ( J:173125 )

decreased urine glucose level ( J:173125 )

increased urine chloride ion level ( J:173125 )

• mice exhibit increased chloride in the urine compared with wild-type mice

decreased urine potassium level ( J:173125 )

decreased urine sodium level ( J:173125 )

increased urine calcium level ( J:173125 )

decreased urine phosphate level ( J:173125 )

decreased urine protein level ( J:173125 )

cellular

increased kidney cell proliferation ( J:173125 )

• mice exhibit increased proliferation of epithelial tubules of the developing kidney papilla compared with wild-type mice