Phenotypes associated with this allele

• Allele Symbol: Tg(Tek-cre)1Arnd
• Allele Name: transgene insertion 1, Bernd Arnold
• Allele ID: MGI:2651392
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gjb2^tm3.1(Gjb1)Kwi/Gjb2^+ Tg(Tek-cre)1Arnd/0	involves: 129P2/OlaHsd * C57BL/6 * CBA * DBA/2 * SJL	MGI:5441206
cn2	Hoxb8^tm3.1(IRES-rtTA2*M2)Mrc/Hoxb8^tm3.1(IRES-rtTA2*M2)Mrc Tg(Tek-cre)1Arnd/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * DBA/2	MGI:4818891
cn3	Tg(JAK2*V617F)FF1Rsko/0 Tg(Tek-cre)1Arnd/0	involves: C57BL/6 * CBA * DBA/2	MGI:5558876

Genotype
MGI:5441206

cn1

• Allelic Composition: Gjb2^{tm3.1(Gjb1)Kwi}/Gjb2⁺; Tg(Tek-cre)1Arnd/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA * DBA/2 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:188626 )

• mice do not exhibit any developmental abnormalities

Genotype
MGI:4818891

cn2

• Allelic Composition: Hoxb8^{tm3.1(IRES-rtTA2*M2)Mrc}/Hoxb8^{tm3.1(IRES-rtTA2*M2)Mrc}; Tg(Tek-cre)1Arnd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * DBA/2

behavior/neurological

behavior/neurological phenotype ( J:162102 )

N • heat insensitivity is not observed in mutants

increased grooming behavior ( J:162102 )

• animals show significantly lengthened grooming times compared to wild-type controls with deletion of Hoxb8 in hematopoietic system; behavior is comparable to complete Hoxb8 null animals

nervous system

nervous system phenotype ( J:162102 )

N • morphology of dorsal spinal cord laminae I and II appears normal, based on labeling for interneurons

integument

focal hair loss ( J:162102 )

• hairless patches develop in most animals due to excessive grooming

Genotype
MGI:5558876

cn3

• Allelic Composition: Tg(JAK2*V617F)FF1Rsko/0; Tg(Tek-cre)1Arnd/0
• Genetic Background: involves: C57BL/6 * CBA * DBA/2

growth/size/body

enlarged spleen ( J:206659 )

• mice develop splenomegaly by 16 weeks of age

hematopoietic system

enlarged spleen ( J:206659 )

• mice develop splenomegaly by 16 weeks of age

abnormal common myeloid progenitor cell morphology ( J:206659 )

• increase in total colony formation, particularly in the number of granulocyte/macrophage (CFU-GM) progenitor cells

abnormal erythropoiesis ( J:206659 )

• decrease in bone marrow erythropoiesis and an increase in splenic erythropoiesis

increased megakaryocyte cell number ( J:206659 )

• CD41/CD61-positive megakaryocytes are increased in the bone marrow and spleen

• greatly increased magakaryopoiesis at 16 weeks of age

abnormal megakaryocyte progenitor cell morphology ( J:206659 )

• increase in total colony formation, particularly in the number of megakaryocyte (CFU-MK) progenitor cells

increased red blood cell distribution width ( J:206659 )

• mice show increased red-cell distribution width without differences in mean cell volume at 22 weeks of age, indicating premyelofibrosis

increased neutrophil cell number ( J:206659 )

• neutrophilia is seen at 8 weeks of age which gets worse by 16 weeks of age

• however no differences in red blood cell or total lymphocyte count is seen

thrombocytosis ( J:206659 )

• thrombocytosis is seen at 8 weeks of age which gets worse by 16 weeks of age

decreased T cell number ( J:206659 )

• reduction in the number of CD3-positive T cells in the bone marrow and spleen

decreased platelet aggregation ( J:206659 )

• blood fails to agglutinate in the presence of ristocetin compared to wild-type

homeostasis/metabolism

abnormal blood homeostasis ( J:206659 )

• plasma levels of von Willebrand Factor (VWF) are normal, however distribution of VWF multimers is different, with mice showing a reduction in ultralarge multimers and a compensatory increase in the levels of smaller VWF multimers

abnormal blood coagulation ( J:206659 )

• blood aggregates quicker and to a greater extent in response to a combination of epinephrine and ADP or collagen than whole blood from wild-type mice, however this is due to increased platelet numbers and not due to increased aggregation and platelets appear to have normal function

decreased platelet aggregation ( J:206659 )

• blood fails to agglutinate in the presence of ristocetin compared to wild-type

decreased susceptibility to induced thrombosis ( J:206659 )

• mice fail to occlude in response to FeCl3 injury over a 30 minute period indicating severely attenuated thrombosis following injury; while platelets adhere to injury site, the platelet plug appears to fail to propagate into an occlusive thrombus

increased bleeding time ( J:206659 )

• increase in bleeding time following injury to the tail compared to wild-type mice

immune system

enlarged spleen ( J:206659 )

• mice develop splenomegaly by 16 weeks of age

increased neutrophil cell number ( J:206659 )

• neutrophilia is seen at 8 weeks of age which gets worse by 16 weeks of age

• however no differences in red blood cell or total lymphocyte count is seen

decreased T cell number ( J:206659 )

• reduction in the number of CD3-positive T cells in the bone marrow and spleen

skeleton

abnormal bone marrow morphology ( J:206659 )

• predominant cell type in the bone marrow is GR1/Mac-1-positive myeloid cells

osteopetrosis ( J:206659 )

• mice develop extensive bone marrow osteopetrosis by 32 weeks of age

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
blood coagulation disease		DOID:1247		J:206659