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Transgene Detail
Symbol: Tg(JAK2*V617F)FF1Rsko
Name: transgene insertion FF1, Radek C Skoda
MGI ID: MGI:5553468
Synonyms: JAK2-V617F
Transgene: Tg(JAK2*V617F)FF1Rsko  Location: unknown  Genetic Position: Chr8, Syntenic
Alliance: Tg(JAK2*V617F)FF1Rsko page
Strain of Origin:  C57BL/6
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(JAK2*V617F)FF1Rsko expresses 1 gene
Mutation detailsThe human BAC, CTD2025A15, containing JAK2 exons 1-12 was combined with a partial cDNA encoding JAK2 exons 13-25 containing the V617F mutation and a polyadenylation signal. To create an inducible Flip-Flop transgene, this construct was modified by inserting a lox66 site into intron 12 and a lox71 site after the polyadenylation signal and the segment between the loxP sites was inverted. Cre-recombination of antiparallel loxP sites flips the orientation of the cDNA and places the intron 12 splice acceptor into the correct position to allow proper splicing of the transgenic pre-mRNA. Recombination generates one wild-type loxP and one double mutant lox66/77 site, which is no longer a substrate for the cre recombinase. This line carries 9 copies of the transgene that integrated in a single chromosomal locus on chromosome 8, band A1. (J:134364)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:134364 Tiedt R, et al., Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood. 2008 Apr 15;111(8):3931-40
All:  13 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
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