Phenotypes associated with this allele

• Allele Symbol: Cul3⁺
• Allele Name: wild type
• Allele ID: MGI:2438334
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Cul3^em1Limi/Cul3^+	C57BL/6N-Cul3^em1Limi	MGI:7492275
ht2	Cul3^tm1Jro/Cul3^+	involves: 129S4/SvJaeSor	MGI:3714760
ht3	Cul3^tm1Suc/Cul3^+	involves: C57BL/6	MGI:5901734
ht4	Cul3^tm1.1Arte/Cul3^+	involves: C57BL/6J * C57BL/6N	MGI:5752860
cn5	Cul3^tm1Jdsr/Cul3^+ Neurod6^tm1(cre)Kan/Neurod6^+ Tg(Thy1-EGFP)MJrs/0	involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J * CBA	MGI:6449730
cn6	Cul3^tm1Jdsr/Cul3^+ Tg(GFAP-cre)25Mes/0	involves: 129S4/SvJaeSor * FVB/N	MGI:6449671

Genotype
MGI:7492275

ht1

• Allelic Composition: Cul3^em1Limi/Cul3⁺
• Genetic Background: C57BL/6N-Cul3^em1Limi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:332937 )

N • no difference in time spent in center versus periphery in open field test

N • normal auditory startle and forelimb grasping milestones

abnormal fear/anxiety-related behavior ( J:332937 )

• longer time to reach cliff avoidance milestone

abnormal response to novel object ( J:332937 )

• shorter time spent investigating novel object in novel object recognition test

abnormal response to social novelty ( J:332937 )

• less time spent sniffing novel mouse in three-chamber test with novel object in the other chamber

• same time spent sniffing novel and familiar mouse in three-chamber test

impaired righting response ( J:332937 )

• longer time to reach surface righting milestone

hyperactivity ( J:332937 )

♂ • longer distance traveled and higher speed in open field test

nervous system

abnormal brain morphology ( J:332937 )

• elongated brain in adults

• reduced relative gray matter volume at age 11 weeks

• increased relative volume of some subcortical regions at age 11 weeks

• normal relative white matter volume at age 11 weeks

decreased brain size ( J:332937 )

• reduced total brain volume at ages P7 and 11 weeks

decreased corpus callosum size ( J:332937 )

• reduced relative volume at age 11 weeks

hypothalamus hyperplasia ( J:332937 )

• at age P7

abnormal entorhinal cortex morphology ( J:332937 )

• reduced relative volume at age 11 weeks

decreased dentate gyrus size ( J:332937 )

• reduced relative volume at age 11 weeks

abnormal somatosensory cortex morphology ( J:332937 )

• reduced relative volume at age 11 weeks

abnormal visual cortex morphology ( J:332937 )

• reduced relative volume of primary visual cortex at age 11 weeks

thin cerebral cortex ( J:332937 )

• at age 11 weeks

• reduced frontal and parieto-temporal cortex thickness at age P7

small olfactory bulb ( J:332937 )

• reduced relative volume at age 11 weeks

small cerebellum ( J:332937 )

• reduced relative volume at ages P7 and 11 weeks

abnormal dendrite morphology ( J:332937 )

• reduced number of neurites on primary cortical neurons from E17.5 embryos

decreased dendritic spine length ( J:332937 )

• on primary cortical neurons from E17.5 embryos

abnormal nervous system electrophysiology ( J:332937 )

• reduced weighted mean firing rate of cultured primary cortical neurons from E17.5 embryos

cellular

abnormal actin cytoskeleton morphology ( J:332937 )

• reduced stability and reduced number of F-actin puncta per dendrite length in cultured adult primary cortical neurons

embryo

embryo phenotype ( J:332937 )

N • viable

decreased embryo size ( J:332937 )

• in E15.5 and E17.5 embryos

growth/size/body

decreased embryo size ( J:332937 )

• in E15.5 and E17.5 embryos

decreased body size ( J:332937 )

• in adults

decreased body weight ( J:332937 )

• in adults

mortality/aging

mortality/aging ( J:332937 )

N • normal lifespan

N • viable

craniofacial

craniofacial phenotype ( J:332937 )

N • normal ear unfolding milestone

reproductive system

reproductive system phenotype ( J:332937 )

N • fertile

vision/eye

vision/eye phenotype ( J:332937 )

N • normal eye opening milestone

Genotype
MGI:3714760

ht2

• Allelic Composition: Cul3^tm1Jro/Cul3⁺
• Genetic Background: involves: 129S4/SvJaeSor

cellular

abnormal cell cycle ( J:122228 )

• mouse embryonic fibroblast cultures have 26% of cells in S phase compared to 15% in wild-type

Genotype
MGI:5901734

ht3

• Allelic Composition: Cul3^tm1Suc/Cul3⁺
• Genetic Background: involves: C57BL/6

homeostasis/metabolism

increased circulating chloride level ( J:226618 )

• blood chloride level is slightly higher

cardiovascular system

cardiovascular system phenotype ( J:226618 )

N • mice exhibit normal blood pressure

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	pseudohypoaldosteronism	DOID:4479		J:226618

Genotype
MGI:5752860

ht4

• Allelic Composition: Cul3^tm1.1Arte/Cul3⁺
• Genetic Background: involves: C57BL/6J * C57BL/6N

cardiovascular system

increased aorta wall thickness ( J:229424 )

hypertension ( J:229424 )

• mice under general anesthesia exhibit higher blood pressure

abnormal blood vessel physiology ( J:229424 )

• the systolic aortic pressure wave is augmented while the diastolic relaxation is slowed, suggesting early wave reflection of the pressure wave and compliance changes indicating vascular stiffness

increased vasoconstriction ( J:229424 )

• maximal constrictor response to intravenous administration of phenylephrine and angiotensin II are higher than in wild-type mice

growth/size/body

decreased body weight ( J:229424 )

decreased body length ( J:229424 )

homeostasis/metabolism

increased circulating aldosterone level ( J:229424 )

increased circulating magnesium level ( J:229424 )

increased circulating phosphate level ( J:229424 )

hyperkalemia ( J:229424 )

increased circulating chloride level ( J:229424 )

• mice exhibit hyperchloremia

abnormal pH regulation ( J:229424 )

• pH is maintained by an increased respiratory drive to increase CO2 removal and hence reduced total CO2 and pCO2 levels

metabolic acidosis ( J:229424 )

• compensated metabolic acidosis

muscle

increased vasoconstriction ( J:229424 )

• maximal constrictor response to intravenous administration of phenylephrine and angiotensin II are higher than in wild-type mice

Genotype
MGI:6449730

cn5

• Allelic Composition: Cul3^tm1Jdsr/Cul3⁺; Neurod6^tm1(cre)Kan/Neurod6⁺; Tg(Thy1-EGFP)MJrs/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J * CBA

behavior/neurological

increased anxiety-related response ( J:292173 )

• fewer entries into and time spent in open arms in elevated plus maze test

• normal total distance travelled and time spent in closed arms in elevated plus maze test

increased thigmotaxis ( J:292173 )

• less time spent in center in open field test

• normal distance traveled in open field test

abnormal response to social novelty ( J:292173 )

• reduced social preference index when introduced to novel mouse and novel inanimate object in three-chamber test

• reduced social preference index when introduced to novel mouse and known mouse in three-chamber test

nervous system

nervous system phenotype ( J:292173 )

N • normal cerebral cortex, corpus callosum and hippocampus morphology and brain weight

N • normal neuron morphology in CA1 and hippocampal regions and in cerebral neocortex layers

N • normal length and complexity of apical and basal dendrites of hippocampal CA1 neurons

N • normal resting membrane potential (RMP) in hippocampal CA1 pyramidal neurons

increased dendritic spine density ( J:292173 )

• increased number of spines on apical dendrites of hippocampal CA1 neurons

increased miniature excitatory postsynaptic current frequency ( J:292173 )

• increased mEPSC frequency in hippocampal CA1 pyramidal neurons

increased miniature inhibitory postsynaptic current frequency ( J:292173 )

• increased mIPSC frequency in hippocampal CA1 pyramidal neurons

mortality/aging

mortality/aging ( J:292173 )

N • viable and normal lifespan

growth/size/body

growth/size/body region phenotype ( J:292173 )

N • normal body size

reproductive system

reproductive system phenotype ( J:292173 )

N • fertile

Genotype
MGI:6449671

cn6

• Allelic Composition: Cul3^tm1Jdsr/Cul3⁺; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S4/SvJaeSor * FVB/N

behavior/neurological

behavior/neurological phenotype ( J:292173 )

N • normal latency to fall in rotarod test

N • normal distance traveled in open field test

N • normal number of arm entries and spontaneous alterations in Y-maze test

N • normal time and number of grooming episodes

increased anxiety-related response ( J:292173 )

• fewer entries into and time spent in open arms in elevated plus maze test

• normal total distance travelled and time spent in closed arms in elevated plus maze test

increased thigmotaxis ( J:292173 )

• less time spent in center in open field test

• normal distance traveled in open field test

abnormal response to social novelty ( J:292173 )

• reduced social preference index when introduced to novel mouse and novel inanimate object in three-chamber test

• reduced social preference index when introduced to novel mouse and known mouse in three-chamber test

nervous system

nervous system phenotype ( J:292173 )

N • normal cerebral cortex, corpus callosum and hippocampus morphology and brain weight

N • normal latency to localize buried food pellets in olfactory sensing test

N • normal neuron morphology in CA1 and CA3 hippocampal regions and in cerebral neocortex layers at age P60

N • normal length and complexity of apical and basal dendrites of hippocampal CA1 neurons at age P60

N • normal resting membrane potential (RMP) in hippocampal CA1 pyramidal neurons at age P60

N • normal number of inhibitory synapses in hippocampal CA1 pyramidal neurons at age P15

increased dendritic spine density ( J:292173 )

• increased number of spines on apical dendrites of hippocampal CA1 neurons at age P15 and P60

abnormal action potential ( J:292173 )

• increased AP frequency in response to injected currents in hippocampal CA1 pyramidal neurons at age P60

abnormal CNS synaptic transmission ( J:292173 )

• higher rate of successful responses and synaptic efficacy to minimal stimulation in hippocampal CA1 pyramidal neurons at age P60

• increased EPSC/IPSC ratio in hippocampal CA1 pyramidal neurons at age P60

• normal synaptic potency to minimal stimulation in hippocampal CA1 pyramidal neurons at age P60

increased miniature excitatory postsynaptic current frequency ( J:292173 )

• increased mEPSC frequency in hippocampal CA1 pyramidal neurons at age P15 and P60

• normal mEPSC amplitude in hippocampal CA1 pyramidal neurons at age P15 and P60

increased miniature inhibitory postsynaptic current frequency ( J:292173 )

• increased mIPSC frequency in hippocampal CA1 pyramidal neurons at age P60

• normal mIPSC amplitude in hippocampal CA1 pyramidal neurons at age P60

decreased paired-pulse facilitation ( J:292173 )

• reduced paired-pulse ratio in hippocampal CA1 pyramidal neurons at age P60

mortality/aging

mortality/aging ( J:292173 )

N • viable and normal lifespan

growth/size/body

growth/size/body region phenotype ( J:292173 )

N • normal body size

reproductive system

reproductive system phenotype ( J:292173 )

N • fertile