Cul3^tm1Suc
Targeted Allele Detail

Summary

• Symbol: Cul3^tm1Suc
• Name: cullin 3; targeted mutation 1, Shinichi Uchida
• MGI ID: MGI:5901731
• Synonyms: Cul3^G(-1)A
• Gene: Cul3 Location: Chr1:80242640-80318197 bp, - strand Genetic Position: Chr1, 41.24 cM, cytoband C4
• Alliance: Cul3^tm1Suc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226618
• Parent Cell Line: Baltha1 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Null/knockout)
• Mutation: Single point mutation
• Mutation details: A mutation in the C terminus of intron 8, c.1207-1G>A, was knocked into the locus along with a floxed neo cassette after exon 9 that was subsequently removed via cre-mediated recombination. This is one of the reported Pseudohypoaldosteronism type II-causing mutations in this gene in humans. RT-PCR and Western blot analysis indicate absence of any truncated forms which would be expected with loss of exon 9 during splicing, indicating a knock-out allele. (J:226618)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cul3 Mutation: 51 strains or lines available

References

• Original: J:226618 Araki Y, et al., Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene. Biol Open. 2015;4(11):1509-17
• All: 1 reference(s)