Phenotypes associated with this allele

• Allele Symbol: Spi1⁺
• Allele Name: wild type
• Allele ID: MGI:2432084
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	\Spi1^tm1Kast/\Spi1^+	B6.129S2-Spi1^tm1Kast	MGI:3044769
ht2	\Spi1^tm2b(EUCOMM)Wtsi/\Spi1^+	C57BL/6N-Spi1^tm2b(EUCOMM)Wtsi/J	MGI:6461694
ht3	\Spi1^tm1Ram/\Spi1^+	involves: 129S2/SvPas	MGI:2658728
cx4	\Spi1^tm1Sing/\Spi1^+ \Spib^tm1Mcs/\Spib^tm1Mcs	involves: 129S/SvEv * 129X1/SvJ	MGI:3609010
cx5	\Spi1^tm1Sing/\Spi1^+ \Spib^tm1Mcs/\Spib^+	involves: 129S/SvEv * 129X1/SvJ	MGI:3609212

Genotype
MGI:3044769

ht1

• Allelic Composition: \Spi1^tm1Kast/\Spi1⁺
• Genetic Background: B6.129S2-Spi1^tm1Kast

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal erythropoiesis ( J:90543 )

• 4 and 5 days after induced erythrocyte lysis spleens from heterozygous mice contain more mature precursors (CFU-Es) and mature erythroid (TER119⁺) cells

• prior to lysis no difference in cell numbers is detected

increased bone marrow cell number ( J:196609 )

increased hematopoietic stem cell number ( J:196609 )

Genotype
MGI:6461694

ht2

• Allelic Composition: \Spi1^{tm2b(EUCOMM)Wtsi}/\Spi1⁺
• Genetic Background: C57BL/6N-Spi1^{tm2b(EUCOMM)Wtsi}/J
• Cell Lines: EPD0923_1_G10

Data Sources

IMPC Data for Spi1

hematopoietic system

decreased erythrocyte cell number ( J:211773 )

IMPC - JAX

increased mean corpuscular volume ( J:211773 )

IMPC - JAX

Genotype
MGI:2658728

ht3

• Allelic Composition: \Spi1^tm1Ram/\Spi1⁺
• Genetic Background: involves: 129S2/SvPas

vision/eye

abnormal eye development ( J:101493 )

• the pupillary membrane and tunica vasculosa lentis partially persist postnatally unlike in wild-type mice due to a defective clearance of apoptotic cells

persistence of hyaloid vascular system ( J:101493 )

• partial

Genotype
MGI:3609010

cx4

• Allelic Composition: \Spi1^tm1Sing/\Spi1⁺; \Spib^tm1Mcs/\Spib^tm1Mcs
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ

hematopoietic system

increased B cell apoptosis ( J:88257 )

• B220⁺ TUNEL<+> splenic lymphocytes are increased 4 times over normal levels

• Annexin A5^hi immature and mature B cells are increased 3 to 4 times over normal levels

• increased splenic B cell apoptosis is found after immunization with the T-dependent antigen DNP-KLH

decreased B cell proliferation ( J:88257 )

• defect in proliferation in response to anti-IgM stimulation is much more severe than in Sfpi1^tm1Sing wild-type Spib^tm1Mcs homozygotes

• 2 to 3 fold reduction in B cell response to LPS although response to PMA and ionomycin is normal and response to anti-CD40 and IL4 is normal

• IgM cross-linking or pervanadate/H₂0₂ stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal

• normal levels of LYN, FYN, BLK, SYK, and BTK are found and in vitro kinase assays of enolase show normal kinase activity in B cells and BLNK, VAV, CD79A, and CD79B are expressed at normal levels with SYK co-precipitating with CD79, but tyrosine phosphorylation of BLNK and PLC gamma is decreased

abnormal B cell differentiation ( J:88257 )

abnormal immature B cell morphology ( J:88257 )

• decreased bone marrow B220^hi/int CD43^- IgM⁺ and B220^hi CD43^- HSA^lo B cells and normal B220^int CD43^- IgM^- and B220^hi CD43^- HSA^hi population indicating a deficiency in immature and mature B cells but normal levels of pro-B and pre-B cells

• E16.5 fetal liver has reduced numbers of B220⁺ CD43^- cells but normal numbers of B220⁺ CD43⁺ cells

• bone marrow has 50% fewer IgM⁺ IgD⁺ and IgM⁺ IgD^- B cells, and also decreased B220⁺ CD43^- B cells and B220⁺ BP-1⁺ B cells

• although B cells from bone marrow and periphery have normal Igl and Igk expression, splenic B cells have 2 to 4 fold higher surface expression of IgM and B cells also express high levels of surface CD19 and CD21

decreased B cell number ( J:88257 )

• B220⁺ IgM⁺ and IgM⁺ IgD⁺ B cells are decreased in number by 50% in spleen and 60%-80% in lymph node

• total numbers of B cells is reduced

absent spleen germinal center ( J:88257 )

• no peanut agglutinin positive germinal centers are found at 10 or 28 days post-immunization

immune system

immune system phenotype ( J:88257 )

N • myeloid lineages appear normal by flow cytometry

N • thymic CD4/CD8 profiles and splenic CD4/CD8 and TCRab/CD3 profiles are normal

increased B cell apoptosis ( J:88257 )

• B220⁺ TUNEL<+> splenic lymphocytes are increased 4 times over normal levels

• Annexin A5^hi immature and mature B cells are increased 3 to 4 times over normal levels

• increased splenic B cell apoptosis is found after immunization with the T-dependent antigen DNP-KLH

decreased B cell proliferation ( J:88257 )

• defect in proliferation in response to anti-IgM stimulation is much more severe than in Sfpi1^tm1Sing wild-type Spib^tm1Mcs homozygotes

• 2 to 3 fold reduction in B cell response to LPS although response to PMA and ionomycin is normal and response to anti-CD40 and IL4 is normal

• IgM cross-linking or pervanadate/H₂0₂ stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal

• normal levels of LYN, FYN, BLK, SYK, and BTK are found and in vitro kinase assays of enolase show normal kinase activity in B cells and BLNK, VAV, CD79A, and CD79B are expressed at normal levels with SYK co-precipitating with CD79, but tyrosine phosphorylation of BLNK and PLC gamma is decreased

abnormal B cell differentiation ( J:88257 )

abnormal immature B cell morphology ( J:88257 )

• decreased bone marrow B220^hi/int CD43^- IgM⁺ and B220^hi CD43^- HSA^lo B cells and normal B220^int CD43^- IgM^- and B220^hi CD43^- HSA^hi population indicating a deficiency in immature and mature B cells but normal levels of pro-B and pre-B cells

• E16.5 fetal liver has reduced numbers of B220⁺ CD43^- cells but normal numbers of B220⁺ CD43⁺ cells

• bone marrow has 50% fewer IgM⁺ IgD⁺ and IgM⁺ IgD^- B cells, and also decreased B220⁺ CD43^- B cells and B220⁺ BP-1⁺ B cells

• although B cells from bone marrow and periphery have normal Igl and Igk expression, splenic B cells have 2 to 4 fold higher surface expression of IgM and B cells also express high levels of surface CD19 and CD21

decreased B cell number ( J:88257 )

• B220⁺ IgM⁺ and IgM⁺ IgD⁺ B cells are decreased in number by 50% in spleen and 60%-80% in lymph node

• total numbers of B cells is reduced

absent spleen germinal center ( J:88257 )

• no peanut agglutinin positive germinal centers are found at 10 or 28 days post-immunization

cellular

increased B cell apoptosis ( J:88257 )

• B220⁺ TUNEL<+> splenic lymphocytes are increased 4 times over normal levels

• Annexin A5^hi immature and mature B cells are increased 3 to 4 times over normal levels

• increased splenic B cell apoptosis is found after immunization with the T-dependent antigen DNP-KLH

decreased B cell proliferation ( J:88257 )

• defect in proliferation in response to anti-IgM stimulation is much more severe than in Sfpi1^tm1Sing wild-type Spib^tm1Mcs homozygotes

• 2 to 3 fold reduction in B cell response to LPS although response to PMA and ionomycin is normal and response to anti-CD40 and IL4 is normal

• IgM cross-linking or pervanadate/H₂0₂ stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal

• normal levels of LYN, FYN, BLK, SYK, and BTK are found and in vitro kinase assays of enolase show normal kinase activity in B cells and BLNK, VAV, CD79A, and CD79B are expressed at normal levels with SYK co-precipitating with CD79, but tyrosine phosphorylation of BLNK and PLC gamma is decreased

Genotype
MGI:3609212

cx5

• Allelic Composition: \Spi1^tm1Sing/\Spi1⁺; \Spib^tm1Mcs/\Spib⁺
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ

hematopoietic system

hematopoietic system phenotype ( J:88257 )

N

decreased B cell proliferation ( J:88257 )

• IgM cross-linking or pervanadate/H202 stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal

abnormal spleen germinal center morphology ( J:88257 )

• although the B cell profiles in spleen and lymph node are normal, there are small splenic germinal centers, and inability to maintain germinal centers

immune system

decreased B cell proliferation ( J:88257 )

• IgM cross-linking or pervanadate/H202 stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal

abnormal spleen germinal center morphology ( J:88257 )

• although the B cell profiles in spleen and lymph node are normal, there are small splenic germinal centers, and inability to maintain germinal centers

cellular

decreased B cell proliferation ( J:88257 )

• IgM cross-linking or pervanadate/H202 stimulation yields less than normal levels of tyrosine phosphorylation and IgM cross-linking also yields less calcium mobilization than normal