Phenotypes associated with this allele
Allelic
Composition |
Dvl2tm1Awb/Dvl2tm1Awb
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Genetic
Background |
either: 129S6/SvEvTac-Dvl2tm1Awb or (involves: 129S6/SvEvTac * NIH Swiss) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
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mortality/aging
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• incomplete penetrance; more than 50% die after E18.5
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cardiovascular system
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• exhibited by several embryos
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• most common defect observed by mice with cardiovascular abnormalities
• frequently observed in conjuction with ventricular septal defects
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• observed in a single embryo
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• frequently observed in conjuction with double outlet right ventricle
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embryo
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• exhibited at E9.5 by 2-3% of mutant mice
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• mild abnormalities in somite segmentation
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homeostasis/metabolism
limbs/digits/tail
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• exhibited by 25% of surviving mice
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• exhibited by some surviving mice
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skeleton
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• observed in one neonate
• affecting the sixth sternebra
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• 90% displayed minor abnormalities
• normal rib number
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• exhibited by surviving mutant mice
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• 90% displayed minor abnormalities
• defects localized dorsally in vertebral ribs and vertebral bodies
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• disorganized thoracic vertebrae
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nervous system
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• exhibited at E9.5 by 2-3% of mutant mice
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• exhibited at E9.5 by 2-3% of mutant mice
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hearing/vestibular/ear
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N |
• normal stereocilia orientation at E18.5
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
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mortality/aging
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• 50% of mice die perinatally
• however, expression of a Dvl3 transgene can rescue perinatal lethality
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mortality/aging
cardiovascular system
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• observed between E14.5 and E18.5
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• observed between E14.5 and E18.5
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• observed between E14.5 and E18.5
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embryo
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• completely open from midbrain to base of tail at E9.5-E10.5
• fusion observed at face and tail
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• mild abnormalities in somite segmentation
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skeleton
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• extenisve fusion along the vertebral column
• defects more severe than those observed in Dvl2tm1Awb homozygous mutants
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• collapsed vertebrae
• defects more severe than those observed in Dvl2tm1Awb homozygous mutants
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nervous system
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• completely open from midbrain to base of tail at E9.5-E10.5
• fusion observed at face and tail
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• increased rows of hair cells within the third of the cochlea near the apex
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• at E18.5 misoriented stereocilia bundles, more severe in the medial region than the base of the organ of Corti with approximately 35% misoriented in the two outer rows of outer hair cells, with denser cellular packing
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hearing/vestibular/ear
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• shorter, wider cochlear ducts
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• increased rows of hair cells within the third of the cochlea near the apex
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• at E18.5 misoriented stereocilia bundles, more severe in the medial region than the base of the organ of Corti with approximately 35% misoriented in the two outer rows of outer hair cells, with denser cellular packing
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hearing/vestibular/ear
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N |
• normal stereocilia orientation at E18.5
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nervous system
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• the floor plate is expanded
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• neural tube fails to close in 100% of embryos
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• the stereociliary bundle orientation in the cochlea is disrupted at 100% penetrance
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• occasionally misaligned inner hair cells are obverse
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hearing/vestibular/ear
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• the stereociliary bundle orientation in the cochlea is disrupted at 100% penetrance
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• occasionally misaligned inner hair cells are obverse
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embryo
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• at the 5- through 8- somite stage, the embryo length to width ratio is reduced
• there is no increase in embryo length to width ratio from 5 to 7 somite stage
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• the floor plate is expanded
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• neural tube fails to close in 100% of embryos
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl1tm1Awb mutation
(2 available);
any
Dvl1 mutation
(35 available)
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2/EGFP)1Awb mutation
(0 available)
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normal phenotype
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• mice are restored to fertile adults
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl1tm1Awb mutation
(2 available);
any
Dvl1 mutation
(35 available)
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2/EGFP)2Awb mutation
(0 available)
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normal phenotype
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• mice are restored to fertile adults
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl1tm1Awb mutation
(2 available);
any
Dvl1 mutation
(35 available)
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2*/EGFP)3Awb mutation
(0 available)
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nervous system
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N |
• neural tube closure defect is rescued
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl1tm1Awb mutation
(2 available);
any
Dvl1 mutation
(35 available)
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2*/EGFP)4Awb mutation
(0 available)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl1tm1Awb mutation
(2 available);
any
Dvl1 mutation
(35 available)
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2*K446M/EGFP)5Awb mutation
(0 available)
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nervous system
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• seen in all mice at E9.5
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• at P0, the stereociliary bundle orientation in the cochlea is disrupted and a less recognizable form of stereocilia is observed
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hearing/vestibular/ear
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• cochlea are wider and shorter
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• at P0, the stereociliary bundle orientation in the cochlea is disrupted and a less recognizable form of stereocilia is observed
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embryo
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• seen in all mice at E9.5
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Allelic
Composition |
Dvl2tm1Awb/Dvl2+
Dvl3tm1Awb/Dvl3+
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Genetic
Background |
involves: 129S6/SvEvTac * Black Swiss |
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cardiovascular system
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• at E18.5, 11 of 28 mice exhibit conotruncal defects
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hearing/vestibular/ear
nervous system
mortality/aging
cardiovascular system
embryo
nervous system
homeostasis/metabolism
cardiovascular system
craniofacial
embryo
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• at E18.5, mice exhibit shortening along the anterior-posterior axis
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growth/size/body
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• one mouse exhibited craniorachischisis, gastroschisis, and an absent tail
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hearing/vestibular/ear
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• mice exhibit rotated stereocillia bundles and mild patterning defects compared to in wild-type mice
• defects are more severe in mice also displaying craniorachischisis
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limbs/digits/tail
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• one mouse exhibited craniorachischisis, gastroschisis, and an absent tail
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nervous system
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• mice exhibit rotated stereocillia bundles and mild patterning defects compared to in wild-type mice
• defects are more severe in mice also displaying craniorachischisis
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mortality/aging
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• Background Sensitivity: 13% survive to adulthood on a mixed background
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hearing/vestibular/ear
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N |
• stereociliary bundle orientation is normal in surviving mice
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nervous system
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• neural tube fails to close in less than 100% of embryos
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embryo
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• neural tube fails to close in less than 100% of embryos
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mortality/aging
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• no mice are recovered at birth
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nervous system
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• similar to that observed in Dvl1tm1Awb Dvl2tm1Awb Vangl2Lp triple homozygotes
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embryo
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• similar to that observed in Dvl1tm1Awb Dvl2tm1Awb Vangl2Lp triple homozygotes
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mortality/aging
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• similar to in Dvl2tm1Awb homozygotes only 50% of expected pups are recovered
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|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dvl2tm1Awb mutation
(1 available);
any
Dvl2 mutation
(33 available)
Tg(Dvl2/EGFP)2Awb mutation
(0 available)
Vangl2Lp mutation
(2 available);
any
Vangl2 mutation
(34 available)
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normal phenotype
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• mice are restored to fertile adults
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embryo
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• severe reduction in the embryo length to width ratio is observed similar to in Dvl1tm1Awb Dvl2tm1Awb Vangl2Lp triple homozygotes
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• similar to that observed in Dvl1tm1Awb Dvl2tm1Awb Vangl2Lp triple homozygotes
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hearing/vestibular/ear
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• shorter, wider chochlear ducts
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• increased rows of hair cells within the third of the cochlea nearest the apex
|
|
|
• misorientation of stereociliary bundles at E18.5
|
|
|
• occasionally misaligned inner hair cells are obverse
|
nervous system
|
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• similar to that observed in Dvl1tm1Awb Dvl2tm1Awb Vangl2Lp triple homozygotes
|
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• increased rows of hair cells within the third of the cochlea nearest the apex
|
|
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• misorientation of stereociliary bundles at E18.5
|
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• occasionally misaligned inner hair cells are obverse
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mortality/aging
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• no mice are recovered at birth
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Analysis Tools