Phenotypes associated with this allele

• Allele Symbol: Raf1^tm1Zim
• Allele Name: targeted mutation 1, Andreas Zimmer
• Allele ID: MGI:2429683
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Raf1^tm1Zim/Raf1^tm1Zim	involves: 129/Sv * 129T2/SvEms	MGI:3758053
hm2	Raf1^tm1Zim/Raf1^tm1Zim	involves: 129/Sv * C57BL/6	MGI:3758054
hm3	Raf1^tm1Zim/Raf1^tm1Zim	involves: 129/Sv * CD-1	MGI:3757851

Genotype
MGI:3758053

hm1

• Allelic Composition: Raf1^tm1Zim/Raf1^tm1Zim
• Genetic Background: involves: 129/Sv * 129T2/SvEms

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:50696 )

• Background Sensitivity: 95% die between E10.5 and E12.5, earlier than on a mixed CD-1 background

growth/size/body

decreased embryo size ( J:50696 )

embryo

embryonic growth arrest ( J:50696 )

• developmental arrest at E10.5, particularly in the posterior region, which often fails to complete axial rotation

decreased embryo size ( J:50696 )

abnormal neural tube morphology ( J:50696 )

• irregular folding of the neural tube

cardiovascular system

distended pericardium ( J:50696 )

• distended pericardium

nervous system

abnormal neural tube morphology ( J:50696 )

• irregular folding of the neural tube

Genotype
MGI:3758054

hm2

• Allelic Composition: Raf1^tm1Zim/Raf1^tm1Zim
• Genetic Background: involves: 129/Sv * C57BL/6

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:50696 )

• Background Sensitivity: 95% die between E10.5 and E12.5, earlier than on a mixed CD-1 background

growth/size/body

decreased embryo size ( J:50696 )

embryo

embryonic growth arrest ( J:50696 )

• developmental arrest at E10.5, particularly in the posterior region, which often fails to complete axial rotation

decreased embryo size ( J:50696 )

abnormal neural tube morphology ( J:50696 )

• irregular folding of the neural tube

cardiovascular system

distended pericardium ( J:50696 )

• distended pericardium

nervous system

abnormal neural tube morphology ( J:50696 )

• irregular folding of the neural tube

Genotype
MGI:3757851

hm3

• Allelic Composition: Raf1^tm1Zim/Raf1^tm1Zim
• Genetic Background: involves: 129/Sv * CD-1

mortality/aging

neonatal lethality, incomplete penetrance ( J:50696 )

• the 2/3 of mutants that reach term die within hours after birth

embryonic lethality during organogenesis, incomplete penetrance ( J:50696 )

• Background Sensitivity: fewer mutants show lethality by E12.5 (44% and 13% in the F2 and F3 generations, respectively) on the CD-1 background than on a 129/Sv or C57BL/6 background

• the number of viable homozygotes (F3 generation) decrease with gestational age, but 2/3 reach term

growth/size/body

embryonic growth retardation ( J:50696 )

• growth retardation is first detectable at E12.5

• developmental delay of 1-1.5 days, indicated by decreased weight and the absence of the ossification centers at E18.5 that are appropriate for this stage

decreased embryo size ( J:50696 )

decreased fetal weight ( J:50696 )

• weight of the oldest surviving mutants (E18.5) is reduced by 1/3

embryo

embryonic growth retardation ( J:50696 )

• growth retardation is first detectable at E12.5

• developmental delay of 1-1.5 days, indicated by decreased weight and the absence of the ossification centers at E18.5 that are appropriate for this stage

decreased embryo size ( J:50696 )

abnormal placenta morphology ( J:50696 )

• arrest in growth of placentas beginning at E10.5

abnormal spongiotrophoblast layer morphology ( J:50696 )

• reduced in size and has a less spongy appearance

abnormal placenta labyrinth morphology ( J:50696 )

• reduced in size, however the giant cell layer appears normal

• number of cytotrophoblast cells is reduced and instead the layer contains large numbers of poorly differentiated mesenchymal cells

abnormal placental labyrinth vasculature morphology ( J:50696 )

• number of blood vessels in the labyrinth layer is reduced

respiratory system

small lung ( J:50696 )

• lungs are smaller due to hypoplasia of parenchymal cells in alveolar septa

abnormal pulmonary alveolus morphology ( J:50696 )

• hypoplasia of parenchymal cells in alveolar septa

respiratory failure ( J:50696 )

• lungs fail to inflate upon birth

vision/eye

eyelids open at birth ( J:50696 )

• 40% of E18.5 and older pups have eyes open

retina fold ( J:50696 )

• 1 out of 4 mutants with open eyelids exhibit a folding of the retina

cellular

decreased fibroblast proliferation ( J:50696 )

• fibroblasts grown in culture show reduced proliferation

integument

underdeveloped hair follicles ( J:50696 )

• hair follicles are less well developed in newborns

small hair follicles ( J:50696 )

abnormal dermal layer morphology ( J:50696 )

• dermis is less differentiated

thin dermal layer ( J:50696 )

abnormal epidermal layer morphology ( J:50696 )

• within the epidermis, the layers are poorly differentiated

abnormal epidermis stratum corneum morphology ( J:50696 )

• keratinization of the stratum corneum is reduced

thin epidermis ( J:50696 )

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:50696 )

• number of blood vessels in the labyrinth layer is reduced