Phenotypes associated with this allele

• Allele Symbol: Pou2af1^tm1Pmt
• Allele Name: targeted mutation 1, Patrick Matthias
• Allele ID: MGI:2388055
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pou2af1^tm1Pmt/Pou2af1^tm1Pmt	involves: 129P2/OlaHsd * C57BL/6	MGI:3800146
cx2	Pou2af1^tm1Pmt/Pou2af1^tm1Pmt Ikzf3^tm1Kge/Ikzf3^tm1Kge	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:2653193
cx3	Fas^lpr/Fas^lpr Pou2af1^tm1Pmt/Pou2af1^tm1Pmt	involves: 129P2/OlaHsd * C57BL/6 * MRL	MGI:3800140

Genotype
MGI:3800146

hm1

• Allelic Composition: Pou2af1^tm1Pmt/Pou2af1^tm1Pmt
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased immunoglobulin level ( J:125114 )

• serum immunoglobulin levels are markedly reduced

hematopoietic system

decreased immunoglobulin level ( J:125114 )

• serum immunoglobulin levels are markedly reduced

Genotype
MGI:2653193

cx2

• Allelic Composition: Pou2af1^tm1Pmt/Pou2af1^tm1Pmt; Ikzf3^tm1Kge/Ikzf3^tm1Kge
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

hematopoietic system

arrested B cell differentiation ( J:81805 )

• blocked transition from pre-B to immature B cells

absent spleen germinal center ( J:81805 )

• failed to form germinal centers

decreased immunoglobulin level ( J:81805 )

• of all types, relative to wild-type controls

• no development of autoantibodies as seen in Zfpn1a3^tm1Kge mutant mice

decreased IgA level ( J:81805 )

decreased IgD level ( J:81805 )

decreased IgE level ( J:81805 )

decreased IgG level ( J:81805 )

decreased IgM level ( J:81805 )

immune system

arrested B cell differentiation ( J:81805 )

• blocked transition from pre-B to immature B cells

absent spleen germinal center ( J:81805 )

• failed to form germinal centers

decreased immunoglobulin level ( J:81805 )

• of all types, relative to wild-type controls

• no development of autoantibodies as seen in Zfpn1a3^tm1Kge mutant mice

decreased IgA level ( J:81805 )

decreased IgD level ( J:81805 )

decreased IgE level ( J:81805 )

decreased IgG level ( J:81805 )

decreased IgM level ( J:81805 )

renal/urinary system

renal/urinary system phenotype ( J:81805 )

N • histological examination of kidneys showed no inflammation, no glomerular immune complex deposition

Genotype
MGI:3800140

cx3

• Allelic Composition: Fas^lpr/Fas^lpr; Pou2af1^tm1Pmt/Pou2af1^tm1Pmt
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * MRL

mortality/aging

mortality/aging ( J:125114 )

N • at 7 months, all double mutants are still alive compared to extensive mortality observed in Fas single mutants by this age

immune system

decreased plasma cell number ( J:125114 )

• number of splenic IgG-secreting cells is dramatically lower than in Fas single mutants

• number of bone marrow IgG-secreting cells is dramatically lower than in Fas single mutants

increased B cell number ( J:125114 )

• in bone marrow, numbers of CD19+IgM- and CD19+IgD- B cells is increased

abnormal T cell number ( J:125114 )

• accumulation of B220+CD3+ cells in spleens and lymph nodes is markedly reduced compared to Fas single mutants

decreased immunoglobulin level ( J:125114 )

• serum immunoglobulin levels are significantly reduced compared to Fas single mutants and are similar to Pou2af1-null mice

• serum level of IgA is reduced but not to same extent as IgG

• IgM production is decreased

autoimmune response ( J:125114 )

• mice do not develop autoantibodies (anti-dsDNA or any IgG antinuclear autoantibodies)

hematopoietic system

hematopoietic system phenotype ( J:125114 )

N • splenic immature and mature B cell numbers are not reduced (a slight increase is observed)

decreased plasma cell number ( J:125114 )

• number of splenic IgG-secreting cells is dramatically lower than in Fas single mutants

• number of bone marrow IgG-secreting cells is dramatically lower than in Fas single mutants

increased B cell number ( J:125114 )

• in bone marrow, numbers of CD19+IgM- and CD19+IgD- B cells is increased

abnormal T cell number ( J:125114 )

• accumulation of B220+CD3+ cells in spleens and lymph nodes is markedly reduced compared to Fas single mutants

decreased immunoglobulin level ( J:125114 )

• serum immunoglobulin levels are significantly reduced compared to Fas single mutants and are similar to Pou2af1-null mice

• serum level of IgA is reduced but not to same extent as IgG

• IgM production is decreased

renal/urinary system

abnormal renal glomerulus morphology ( J:125114 )

• no IgG or C3 deposition is observed, but crescent formation and enlargement of glomeruli is observed

• no inflammation is observed

glomerular crescent ( J:125114 )

• crescent formation is observed

renal glomerulus hypertrophy ( J:125114 )

• enlargement of glomeruli is observed