Phenotypes associated with this allele

• Allele Symbol: Vcam1^tm2Flv
• Allele Name: targeted mutation 2, Richard A Flavell
• Allele ID: MGI:2386556
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Vcam1^tm2Flv/Vcam1^tm3Flv Tg(Tek-cre)12Flv/0	involves: 129S1/Sv * C3H * C57BL/6	MGI:3845662
cn2	Vcam1^tm2Flv/Vcam1^tm2Flv Tg(Mx1-cre)1Cgn/0	involves: 129S1/Sv * C57BL/6 * CBA	MGI:3845664

Genotype
MGI:3845662

cn1

• Allelic Composition: Vcam1^tm2Flv/Vcam1^tm3Flv; Tg(Tek-cre)12Flv/0
• Genetic Background: involves: 129S1/Sv * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:107447 )

• weigh about 10% less than controls

spleen hyperplasia ( J:107447 )

• modest but significant increase in spleen cellularity

immune system

spleen hyperplasia ( J:107447 )

• modest but significant increase in spleen cellularity

decreased B cell number ( J:68147 )

• decrease in the number of IgD+ B cells in the bone marrow

decreased immature B cell number ( J:68147 )

• decrease in the number of IgD^loIgM^hi immature B cells in the bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:68147 )

• the fraction of naive CD4+ T cells is decreased in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:68147 )

• decrease in the number of CD8+ T cells in the bone marrow

increased leukocyte cell number ( J:68147 , J:107447 )

• mild (J:68147)

• increased WBC number with a higher proportion of B220+ cells and a lower proportion of Mac-1+ cells (J:107447)

increased immature B cell number ( J:68147 )

• increase in the number of B220+IgD^loIgM^hi immature B cells in the periphery

abnormal splenic cell ratio ( J:107447 )

• increase in the number of progenitor cells

abnormal lymphocyte physiology ( J:68147 )

• impaired lymphocyte short term migration to the bone marrow

• however, migration to the spleen and lymph nodes is similar to controls

abnormal B cell physiology ( J:68147 )

• impaired short term migration of IgD+ cells to the bone marrow

abnormal CD4-positive, alpha-beta T cell physiology ( J:68147 )

• impaired short term migration of CD4+ cells to the bone marrow

abnormal CD8-positive, alpha-beta T cell physiology ( J:68147 )

• impaired short term migration of CD8+ cells to the bone marrow

hematopoietic system

spleen hyperplasia ( J:107447 )

• modest but significant increase in spleen cellularity

abnormal blood cell morphology/development ( J:107447 , J:123142 )

• increase in the number of progenitor cells in the peripheral blood in young and old mice (J:107447)

• the number of colony forming unit cultures in the peripheral blood is increased compared to controls (J:123142)

abnormal bone marrow cell morphology/development ( J:107447 , J:123142 )

• treatment with anti-VLA4 antibody fails to mobilize progenitor cells from the bone marrow to the peripheral blood (J:107447)

• in response to granulocyte colony stimulating factor (G-CSF) more mobilized progenitors are found in the peripheral blood and fewer progenitors are found in the spleen compared to controls (J:107447)

• after G-CSF treatment or G-CSF plus Flt3 ligand treatment total progenitor cell numbers from all hematopoietic organs are decreased compared to controls (J:107447)

• treatment with anti-alpha4 antibody fails to mobilize progenitor cells from the bone marrow to the peripheral blood (J:123142)

• following irradiation, homing of wild-type donor cells to the bone marrow is impaired (J:123142)

decreased bone marrow cell number ( J:68147 , J:107447 )

• decrease in the number of IgD^loIgM^hi immature B cells, IgD+ B cells and CD8+ T cells in the bone marrow (J:68147)

• the fraction of naive CD4+ T cells is decreased (J:68147)

• however, no decrease in the number of pro-B cells is detected in the bone marrow and the total number of bone marrow cells is similar to controls (J:68147)

• in both young (9 - 14 weeks of age) and old (over 40 weeks of age) mice (J:107447)

• moderate decrease in progenitor content in young but not in old mice (J:107447)

decreased B cell number ( J:68147 )

• decrease in the number of IgD+ B cells in the bone marrow

decreased immature B cell number ( J:68147 )

• decrease in the number of IgD^loIgM^hi immature B cells in the bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:68147 )

• the fraction of naive CD4+ T cells is decreased in the bone marrow

decreased CD8-positive, alpha-beta T cell number ( J:68147 )

• decrease in the number of CD8+ T cells in the bone marrow

increased leukocyte cell number ( J:68147 , J:107447 )

• mild (J:68147)

• increased WBC number with a higher proportion of B220+ cells and a lower proportion of Mac-1+ cells (J:107447)

increased immature B cell number ( J:68147 )

• increase in the number of B220+IgD^loIgM^hi immature B cells in the periphery

abnormal splenic cell ratio ( J:107447 )

• increase in the number of progenitor cells

abnormal lymphocyte physiology ( J:68147 )

• impaired lymphocyte short term migration to the bone marrow

• however, migration to the spleen and lymph nodes is similar to controls

abnormal B cell physiology ( J:68147 )

• impaired short term migration of IgD+ cells to the bone marrow

abnormal CD4-positive, alpha-beta T cell physiology ( J:68147 )

• impaired short term migration of CD4+ cells to the bone marrow

abnormal CD8-positive, alpha-beta T cell physiology ( J:68147 )

• impaired short term migration of CD8+ cells to the bone marrow

Genotype
MGI:3845664

cn2

• Allelic Composition: Vcam1^tm2Flv/Vcam1^tm2Flv; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S1/Sv * C57BL/6 * CBA

hematopoietic system

abnormal blood cell morphology/development ( J:123142 )

• after pIpC injection, the number of colony forming unit cultures in the peripheral blood is significantly but variably increased compared to controls

• the degree of increase in colony forming unit cultures correlates to the extent of recombination in bone marrow nonhematopoietic cells

decreased bone marrow cell number ( J:123142 )

• after pIpC injection, progenitor cell numbers and total cell numbers are decreased compared to controls