Phenotypes associated with this allele

• Allele Symbol: Tg(EEF1A1-SHC1*)1Ravi
• Allele Name: transgene insertion 1, Kodi S Ravichandran
• Allele ID: MGI:2384188
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(EEF1A1-SHC1*)1Ravi/? Tg(Lck-cre)1Cwi/?	involves: 129 * C57BL/6	MGI:3851559
cn2	Tg(EEF1A1-SHC1*)1Ravi/0 Tg(Nes-cre)1Kln/0	involves: 129 * C57BL/6 * SJL	MGI:3851591

Genotype
MGI:3851559

cn1

• Allelic Composition: Tg(EEF1A1-SHC1*)1Ravi/?; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• mice have a 83% DN3 stage thymocytes compared to 48% in normal mice, with a concomitant decrease in the DN4 stage thymocytes (46% in normal versus 8% in double-transgenic mice)

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

hematopoietic system

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

abnormal double-negative T cell morphology ( J:78180 )

• double-negative thymocyte numbers are similar to wild-type but the distribution of the sub-populations differs

• mice have a 83% DN3 stage thymocytes compared to 48% in normal mice, with a concomitant decrease in the DN4 stage thymocytes (46% in normal versus 8% in double-transgenic mice)

• these DN3 cells have less proliferation suggesting a partial block in development

decreased double-positive T cell number ( J:78180 )

• double-positive thymocyte numbers are decreased compared to wild-type while double-negative numbers are close to normal

decreased single-positive T cell number ( J:78180 )

• single-positive thymocyte numbers are decreased compared to controls

endocrine/exocrine glands

thymus hypoplasia ( J:78180 )

• thymus cellularity is only 10-30% of controls

Genotype
MGI:3851591

cn2

• Allelic Composition: Tg(EEF1A1-SHC1*)1Ravi/0; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129 * C57BL/6 * SJL

growth/size/body

decreased body weight ( J:111058 )

• at birth and during early postnatal development, there is a slight body weight difference

• the biggest differences occur at 21 days of age

• later in life males have bigger body weight differences than females

nervous system

increased neuron apoptosis ( J:111058 )

• apoptosis among neural progenitors is increased during embryonic brain development

• apoptosis is noted in the frontal cortex at E12.5 and in the pons at E10.5 and E12.5

decreased brain size ( J:111058 )

• brain weight is less than controls from 2 days of age to 1 year in age

• the biggest differences occur at 21 days of age

decreased striatum size ( J:111058 )

• the stratium area is smaller at 2 days of age than controls and becomes even smaller at 21 days of age

abnormal primary somatosensory cortex morphology ( J:111058 )

• the entire thickness of the primary somatosensory cortex is reduced to less than 85% of controls

• layers II through IV are more dramatically affected (reduced by less 60% of controls in layer IV) than layers V-VI (reduced by 92% of controls)

thin cerebral cortex ( J:111058 )

• the cerebral cortex is thinner at 2 days of age than controls and becomes even thinner at 21 days of age

• at 2 days of age, the cerebral cortex is 58% smaller than controls which is more affected than other parts of the brain

small olfactory bulb ( J:111058 )

• olfactory bulb is 82% of controls at 2 days of age

abnormal postnatal subventricular zone morphology ( J:111058 )

• the SVZ area is smaller at 2 days of age than controls and becomes even smaller at 21 days of age

decreased neuronal precursor cell number ( J:111058 )

• apoptosis among neural progenitors is increased during embryonic brain development

• proliferation of neural progenitors is not changed so presumably there are less progenitor cells in the developing brain

cellular

increased neuron apoptosis ( J:111058 )

• apoptosis among neural progenitors is increased during embryonic brain development

• apoptosis is noted in the frontal cortex at E12.5 and in the pons at E10.5 and E12.5