About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rad54ltm1Jhjh
targeted mutation 1, Jan H J Hoeijmakers
MGI:2183934
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rad54ltm1Jhjh/Rad54ltm1Jhjh involves: 129P2/OlaHsd MGI:4429605
hm2
 		Rad54ltm1Jhjh/Rad54ltm1Jhjh involves: 129P2/OlaHsd * C57BL/6 MGI:3040059
cx3
 		Prkdctm1Get/Prkdc+
Rad54ltm1Jhjh/Rad54ltm1Jhjh 		involves: 129P2/OlaHsd * C57BL/6 MGI:3040060
cx4
 		Prkdctm1Get/Prkdctm1Get
Rad54ltm1Jhjh/Rad54ltm1Jhjh 		involves: 129P2/OlaHsd * C57BL/6 MGI:3040061
cx5
 		Rad54btm1Roka/Rad54btm1Roka
Rad54ltm1Jhjh/Rad54ltm1Jhjh 		involves: 129P2/OlaHsd * C57BL/6 MGI:3618235
cx6
 		Rad52tm1Aps/Rad52tm1Aps
Rad54ltm1Jhjh/Rad54ltm1Jhjh 		involves: 129P2/OlaHsd * C57BL/6 MGI:3850728


Genotype
MGI:4429605
hm1
Allelic
Composition		 Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:47993 )
N
• somatic hypermutation rates in memory B cells are normal




Genotype
MGI:3040059
hm2
Allelic
Composition		 Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:101697 )
• following treatment with mitomycine C, mice exhibit increased lethality compared with similarly treated wild-type mice

cellular
abnormal chromosome morphology ( J:101697 )
• following mitomycine C treatment, mice exhibit a small reduction in the frequency of sister chromatid exchange compared with similarly treated wild-type mice
abnormal cell physiology ( J:83373 )
• mice were sensitive to DNA crosslinking agents and to ionizing radiation
• there was no affect on somatic instability
increased cellular sensitivity to gamma-irradiation ( J:105544 )
• derived ES cells show a 2-3X increase in sensitivity to ionizing radiation

hematopoietic system
abnormal definitive hematopoiesis ( J:101697 )
• following treatment with X-rays, mice exhibit a small hematopoietic depression unlike similarly treated wild-type mice

immune system
immune system phenotype ( J:39642 )
N
• mice were specifically examined for an effect on V(D)J recombination and none was found
• at the same time, no other gross abnormalities were observed

homeostasis/metabolism
increased susceptibility to xenobiotic induced morbidity/mortality ( J:101697 )
• following treatment with mitomycine C, mice exhibit increased lethality compared with similarly treated wild-type mice




Genotype
MGI:3040060
cx3
Allelic
Composition		 Prkdctm1Get/Prkdc+
Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkdctm1Get mutation (3 available); any Prkdc mutation (408 available)
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal chromosome morphology ( J:84801 )
• end to end chromosome fusions occur in about 1.6 out of 100 metaphases
• chromosome fusions involve random chromosomes
decreased telomere length ( J:84801 )
• telomeres are significantly shorter than in doubly heterozygous controls but there is always a detectable telomere




Genotype
MGI:3040061
cx4
Allelic
Composition		 Prkdctm1Get/Prkdctm1Get
Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkdctm1Get mutation (3 available); any Prkdc mutation (408 available)
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal chromosome morphology ( J:84801 )
• end to end chromosome fusions occur in about 3 out of 50 metaphases
• chromosome fusions involve random chromosomes




Genotype
MGI:3618235
cx5
Allelic
Composition		 Rad54btm1Roka/Rad54btm1Roka
Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad54btm1Roka mutation (0 available); any Rad54b mutation (143 available)
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:105544 )
• mice extremely sensitive to mitomycin C, 100% mortality when given a 7.5mg/kg dose

cellular
abnormal cell physiology ( J:105544 )
• virtually no homologous recombination occurs in derived ES cells
increased cellular sensitivity to gamma-irradiation ( J:105544 )
• derived ES cells show a 2-3X increase in sensitivity to ionizing radiation which is not seen in the mice themselves

homeostasis/metabolism
increased susceptibility to xenobiotic induced morbidity/mortality ( J:105544 )
• mice extremely sensitive to mitomycin C, 100% mortality when given a 7.5mg/kg dose

hematopoietic system
abnormal erythrocyte morphology ( J:105544 )
• much higher numbers of micronuclei after treatment with mitomycin C




Genotype
MGI:3850728
cx6
Allelic
Composition		 Rad52tm1Aps/Rad52tm1Aps
Rad54ltm1Jhjh/Rad54ltm1Jhjh
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad52tm1Aps mutation (1 available); any Rad52 mutation (18 available)
Rad54ltm1Jhjh mutation (0 available); any Rad54l mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:101697 )
• following treatment with mitomycine C, mice exhibit increased lethality compared with similarly treated wild-type mice and Rad54ltm1Jhjh homozygotes

hematopoietic system
abnormal definitive hematopoiesis ( J:101697 )
• following treatment with X-rays, mice exhibit severe hematopoietic depression compared with similarly treated wild-type mice and Rad54ltm1Jhjh homozygotes

cellular
abnormal chromosome morphology ( J:101697 )
• following mitomycine C treatment, mice exhibit a small reduction in the frequency of sister chromatid exchange compared with similarly treated wild-type mice

homeostasis/metabolism
increased susceptibility to xenobiotic induced morbidity/mortality ( J:101697 )
• following treatment with mitomycine C, mice exhibit increased lethality compared with similarly treated wild-type mice and Rad54ltm1Jhjh homozygotes




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory