Rad54l^tm1Jhjh
Targeted Allele Detail

Summary

• Symbol: Rad54l^tm1Jhjh
• Name: RAD54 like (S. cerevisiae); targeted mutation 1, Jan H J Hoeijmakers
• MGI ID: MGI:2183934
• Synonyms: mRAD54-, mRAD54^307neo
• Gene: Rad54l Location: Chr4:115951461-115980887 bp, - strand Genetic Position: Chr4, 53.1 cM
• Alliance: Rad54l^tm1Jhjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39642
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 4, upstream of approximately 90% of the protein product including the conserved motifs involved in ATPase activity. Sequence analysis showed that excision of the disrupted exon would result in a frameshift mutation. The absence of transcript and protein was determined by Northern and Western blot analysis. (J:39642)

Tumor Data

List all tumor models in MMHCdb carrying Rad54l^tm1Jhjh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad54l Mutation: 46 strains or lines available

References

• Original: J:39642 Essers J, et al., Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell. 1997 Apr 18;89(2):195-204
• All: 23 reference(s)