Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
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immune system
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• LPS-induced production of tristetraprolin and TNF is reduced compared to wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
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immune system
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• macrophage foam cell formation is significantly reduced after macrophage incubation with oxLDL
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• germinal centers are significantly enlarged compared to controls after immunization with TNP-KLH due to decreased apoptosis in the region
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• LPS-induced TNF-alpha and IFN-gamma release is reduced in cultured spleen cells, however this is not due to reduced levels or stability of mRNA or impaired secretion but reduced post-transcriptional biosynthesis
• LPS-induced IL-1beta and IL-6 release is reduced in cultured spleen cells due to a reduction in mRNA levels
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• serum TNF-alpha levels are reduced by more than 90% following LPS challenge compared to controls
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• increased survival after LPS-induced endotoxic shock due to a drastic reduction of Tnf-alpha biosynthesis, not to impaired TNF receptor signaling
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cardiovascular system
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• following ischemia-reperfusion myocardial injury, homozygotes display a significant reduction in the number of apoptotic cardiomyocytes relative to wild-type mice
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• homozygotes display no significant differences in baseline cardiac function relative to wild-type mice
• however, homozygotes are resistant to ischemic reperfusion myocardial injury, with a significantly enhanced post-ischemic recovery of left ventricular function relative to wild-type mice
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• following ischemia-reperfusion myocardial injury, homozygotes exhibit a ~20% reduction in myocardial infarct size relative to non-preconditioned wild-type mice
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muscle
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• following ischemia-reperfusion myocardial injury, homozygotes display a significant reduction in the number of apoptotic cardiomyocytes relative to wild-type mice
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homeostasis/metabolism
hematopoietic system
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• macrophage foam cell formation is significantly reduced after macrophage incubation with oxLDL
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• germinal centers are significantly enlarged compared to controls after immunization with TNP-KLH due to decreased apoptosis in the region
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cellular
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• following ischemia-reperfusion myocardial injury, homozygotes display a significant reduction in the number of apoptotic cardiomyocytes relative to wild-type mice
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• macrophage foam cell formation is significantly reduced after macrophage incubation with oxLDL
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
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mortality/aging
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• in response to i.v. injection with 1.5 x 105 CFU/kg virulent L. monocytogenes, only 1 of 7 homozygotes (vs 9 of 10 wild-type controls) survive the 14-day observation period post-infection
• however, all homozygotes survive L. monocytogenes infection with 104 CFU/kg
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immune system
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• in an optimized quantitative phagocytosis assay, mutant bone marrow-derived macrophages (BMDMs) display reduced uptake of rhodamine-labeled E. coli particles relative to wild-type BMDMs
• however, macrophage generation of NO and oxidative burst activity in response to L. monocytogenes are both normal
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• in vitro, isolated spleen cells from homozygous mutant mice show strongly diminished IFN-gamma production in response to stimulation with heat-killed L. monocytogenes (HKLM)
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• in response to stimulation with HKLM, mutant BMDMs display an enhanced IL-1 beta release relative to similarly-treated wild-type BMDMs
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• at 72 hrs post-infection with 1.5 x 105 CFU/kg virulent L. monocytogenes, homozygotes display strongly reduced TNF levels in spleen relative to wild-type controls
• in response to stimulation with LPS, lipoteichoic acid (LTA), E. coli, and HKLM, mutant BMDMs display strongly reduced TNF release relative to similarly-treated wild-type BMDMs
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• at 72 hrs (but not at 48 hrs) post-infection with 1.5 x 105 CFU/kg virulent L. monocytogenes, homozygotes display a strongly increased bacterial load in spleen and lung relative to wild-type controls, with no differences in total blood leukocyte numbers
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• in response to i.v. injection with 1.5 x 105 CFU/kg virulent L. monocytogenes, only 1 of 7 homozygotes (vs 9 of 10 wild-type controls) survive the 14-day observation period post-infection
• however, all homozygotes survive L. monocytogenes infection with 104 CFU/kg
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hematopoietic system
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• in an optimized quantitative phagocytosis assay, mutant bone marrow-derived macrophages (BMDMs) display reduced uptake of rhodamine-labeled E. coli particles relative to wild-type BMDMs
• however, macrophage generation of NO and oxidative burst activity in response to L. monocytogenes are both normal
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cellular
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• in an optimized quantitative phagocytosis assay, mutant bone marrow-derived macrophages (BMDMs) display reduced uptake of rhodamine-labeled E. coli particles relative to wild-type BMDMs
• however, macrophage generation of NO and oxidative burst activity in response to L. monocytogenes are both normal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ldlrtm1Her mutation
(19 available);
any
Ldlr mutation
(76 available)
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
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cardiovascular system
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• reduced development of atherosclerosis after 8-16 weeks on an atherogenic diet
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hematopoietic system
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• reduced in number among peritoneal macrophage in mice fed an atherogenic diet for 10 weeks
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• decreased macrophage content in longitudinal sections of the aortic arch
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homeostasis/metabolism
immune system
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• reduced in number among peritoneal macrophage in mice fed an atherogenic diet for 10 weeks
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• decreased macrophage content in longitudinal sections of the aortic arch
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cellular
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• reduced in number among peritoneal macrophage in mice fed an atherogenic diet for 10 weeks
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
Mapkapk3tm1.1Lex mutation
(0 available);
any
Mapkapk3 mutation
(22 available)
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mortality/aging
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N |
• in contrast to expectations, mice are viable
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immune system
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• LPS-induced production of tristetraprolin and TNF is reduced compared to wild-type mice and compared to Mapkapk2 single homozygotes
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embryo
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N |
• in contrast to expectations, no defects in embryonic development are detected
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapkapk2tm1Mgl mutation
(0 available);
any
Mapkapk2 mutation
(36 available)
Tnftm2Gkl mutation
(1 available);
any
Tnf mutation
(46 available)
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mortality/aging
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• increase in mortality from 8 weeks of age onward
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cellular
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• mice have fewer apoptotic cells in inflamed ileal tissue compared to Tnftm2Gkl/+ controls
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immune system
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N |
• there is no change in CD4+ to CD8+ ratios compared to findings in Tnftm2Gkl/+ single mutants
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• Tnftm2Gkl phenotype is exacerbated in these mice
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• IBD is associated with early formation of multiple granulomas
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digestive/alimentary system
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• Tnftm2Gkl phenotype is exacerbated in these mice
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Analysis Tools
