All Phenotypes Itk<tm1Ljb> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itk^tm1Ljb/Itk^tm1Ljb	B10.129-Itk^tm1Ljb	MGI:4354588
hm2	Itk^tm1Ljb/Itk^tm1Ljb	B6.129-Itk^tm1Ljb	MGI:4354503
hm3	Itk^tm1Ljb/Itk^tm1Ljb	involves: 129 * C57BL/6	MGI:4354505
cx4	Itk^tm1Ljb/Itk^tm1Ljb Txk^tm1Pls/Txk^tm1Pls	B6.129-Txk^tm1Pls Itk^tm1Ljb	MGI:4354591
cx5	Itk^tm1Ljb/Itk^tm1Ljb Rag1^tm1Mom/Rag1^tm1Mom Tg(Tcra5CC7,Tcrb5CC7)IWep/?	involves: 129 * C57BL/10	MGI:4354514
cx6	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra5CC7,Tcrb5CC7)IWep/?	involves: 129 * C57BL/10	MGI:4354593
cx7	Itk^tm1Ljb/Itk^tm1Ljb Tg(TcraTcrb)1100Mjb/?	involves: 129 * C57BL/6 * C57BL/10	MGI:4354589
cx8	Il15^tm1Imx/Il15^tm1Imx Itk^tm1Ljb/Itk^tm1Ljb	involves: 129 * C57BL/6 * C57BL/10	MGI:4354590
cx9	Itk^tm1Ljb/Itk^tm1Ljb Tcrd^tm1Mom/Tcrd^tm1Mom	involves: 129 * C57BL/6 * C57BL/10	MGI:4354603
cx10	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354594
cx11	H2^b/H2^b Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354597
cx12	H2^b/H2^k Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354598

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:113408 )

• CD8⁺CD44^hi thymocytes first appear at 4 days after birth

• these CD44^hiCD8⁺ thymocytes accumulate with age

• increased numbers of CD8⁺ SP thymocytes is accompanied by a transient deficit in the numbers of peripheral CD8⁺ T cells

increased germinal center B cell number ( J:148531 )

• germinal center B cells are increased almost two-fold compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• percentage of CD4 single positive thymoyctes is slightly decreased compared to controls

• the percentage of CD4⁺ T cells in the periphery is half that of controls

increased CD8-positive, alpha-beta T cell number ( J:113408 , J:123795 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus (J:113408)

• percentage of CD8 single positive thymocyotes are increased about 2-fold compared to controls (J:123795)

• these thymocytes have a memory phenotype based on CD44 expression (J:123795)

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal gamma-delta T cell morphology ( J:148531 )

• gamma-delta T cells have increased expression of CD4, NK1.1, CD44

• gamma-delta T cells also have increased expression ICOS that can promote B cell differentiation

• after activation, gamma-delta T cells increase expression of CD40L and OX40 that also promote B cell differentation

increased mature gamma-delta T cell number ( J:148531 )

• gamma delta T cell numbers are increased in the thymus, spleen, lymph nodes, and liver but not in the intestinal epithelium

• increases range 2 to 3 fold with a disproportionate increase in the Vgamma1.1 subset

increased IgE level ( J:148531 )

• IgE levels are highly elevated at 10 micrograms per ml compared to virtually none in controls

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

decreased interferon-gamma secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells have decreased IFN-gamma secretion after activation compared to controls

increased interferon-gamma secretion ( J:113408 )

• CD8⁺ T cells have increased secretion of IFN-gamma upon ex vivo stimulation by PMA and ionomycin

increased interleukin-10 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-10 on a per cell basis than controls after activation

increased interleukin-13 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-13 on a per cell basis than controls after activation

increased interleukin-4 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-4 on a per cell basis than controls after activation

• CD4⁺ gamma delta T cells also produce more IL-4 than their wild-type counterparts

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:113408 )

• CD8⁺CD44^hi thymocytes first appear at 4 days after birth

• these CD44^hiCD8⁺ thymocytes accumulate with age

• increased numbers of CD8⁺ SP thymocytes is accompanied by a transient deficit in the numbers of peripheral CD8⁺ T cells

increased germinal center B cell number ( J:148531 )

• germinal center B cells are increased almost two-fold compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• percentage of CD4 single positive thymoyctes is slightly decreased compared to controls

• the percentage of CD4⁺ T cells in the periphery is half that of controls

increased CD8-positive, alpha-beta T cell number ( J:113408 , J:123795 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus (J:113408)

• percentage of CD8 single positive thymocyotes are increased about 2-fold compared to controls (J:123795)

• these thymocytes have a memory phenotype based on CD44 expression (J:123795)

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal gamma-delta T cell morphology ( J:148531 )

• gamma-delta T cells have increased expression of CD4, NK1.1, CD44

• gamma-delta T cells also have increased expression ICOS that can promote B cell differentiation

• after activation, gamma-delta T cells increase expression of CD40L and OX40 that also promote B cell differentation

increased mature gamma-delta T cell number ( J:148531 )

• gamma delta T cell numbers are increased in the thymus, spleen, lymph nodes, and liver but not in the intestinal epithelium

• increases range 2 to 3 fold with a disproportionate increase in the Vgamma1.1 subset

increased IgE level ( J:148531 )

• IgE levels are highly elevated at 10 micrograms per ml compared to virtually none in controls

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	B6.129-Itk^tm1Ljb

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal NK T cell morphology ( J:120689 )

• there is a partial block in NK T cell development in the thymus

• mice have twice the percentage of fraction 2 (Dex5⁺, NK1.1^-) cells as controls, with a concomitant reduction in fraction 3 Dex5⁺, NK1.1⁺cells

• the percentage of CD4⁺CD8⁺ double positive NK precursors is about 10-fold less than controls in the thymus

decreased NK T cell number ( J:120689 )

• NK T cells found in the spleen of 6-8 week old mice is about half that of controls

• by 20 weeks of age, NK T cell numbers have dropped to about a third of controls

• thymic numbers of mature NK T cells is 2-fold above normal at 6-8 weeks of age but then drops to a third of normal by 20 weeks of age

hematopoietic system

abnormal NK T cell morphology ( J:120689 )

• there is a partial block in NK T cell development in the thymus

• mice have twice the percentage of fraction 2 (Dex5⁺, NK1.1^-) cells as controls, with a concomitant reduction in fraction 3 Dex5⁺, NK1.1⁺cells

• the percentage of CD4⁺CD8⁺ double positive NK precursors is about 10-fold less than controls in the thymus

decreased NK T cell number ( J:120689 )

• NK T cells found in the spleen of 6-8 week old mice is about half that of controls

• by 20 weeks of age, NK T cell numbers have dropped to about a third of controls

• thymic numbers of mature NK T cells is 2-fold above normal at 6-8 weeks of age but then drops to a third of normal by 20 weeks of age

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:48231 )

• CD4⁺ T cell numbers are about half that of controls

abnormal T cell activation ( J:48231 )

• CD4⁺ T cells have less increases in intracellular calcium after high doses of anti-CD3 than controls

• at sub-optimal doses of anti-CD3, CD4⁺ T cells fail to flux any calcium

• calcium influx is to due defects in opening of plasma membrane channels and not from defects in internal calcium stores

decreased interleukin-2 secretion ( J:48231 )

• CD4⁺ T cell produce only half the amount of IL-2 as controls when stimulated with anti-CD3 and anti-CD28

• when PMA and ionomycin are used to activate cells IL-2 production is comparable to controls

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:48231 )

• CD4⁺ T cell numbers are about half that of controls

abnormal T cell activation ( J:48231 )

• CD4⁺ T cells have less increases in intracellular calcium after high doses of anti-CD3 than controls

• at sub-optimal doses of anti-CD3, CD4⁺ T cells fail to flux any calcium

• calcium influx is to due defects in opening of plasma membrane channels and not from defects in internal calcium stores

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Txk^tm1Pls/Txk^tm1Pls
Genetic Background	B6.129-Txk^tm1Pls Itk^tm1Ljb

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Txk^tm1Pls mutation (0 available); any Txk mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased CD8-positive, alpha-beta T cell number ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus

increased interferon-gamma secretion ( J:113408 )

• CD8⁺ T cells have increased secretion of IFN-gamma upon ex vivo stimulation by PMA and ionomycin

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Rag1^tm1Mom/Rag1^tm1Mom Tg(Tcra5CC7,Tcrb5CC7)IWep/?
Genetic Background	involves: 129 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Rag1^tm1Mom mutation (49 available); any Rag1 mutation (120 available)
Tg(Tcra5CC7,Tcrb5CC7)IWep mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD4-positive T cell differentiation ( J:93606 )

• when stimulated with low concentrations of antigen, CD4 T cells differentiate predominately into a Th1 phenotype while controls develop into a Th2 phenotype

• these T cells have increased expression of the T-bet transcription factor

decreased interferon-gamma secretion ( J:93606 )

• T cells cultured under Th1 polarizing conditions produce less IFN-gamma than controls

• Th1 T cells produce 1.5- to 200-fold less IFN-gamma than controls

decreased interleukin-10 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-10 than controls

decreased interleukin-4 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-4 than controls

• Th2 T cells produce 3.5- to 300-fold less IL-4 than controls

decreased interleukin-5 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-5 than controls

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:93606 )

• when stimulated with low concentrations of antigen, CD4 T cells differentiate predominately into a Th1 phenotype while controls develop into a Th2 phenotype

• these T cells have increased expression of the T-bet transcription factor

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra5CC7,Tcrb5CC7)IWep/?
Genetic Background	involves: 129 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tg(Tcra5CC7,Tcrb5CC7)IWep mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(TcraTcrb)1100Mjb/?
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:113408 )

N	• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in Itk^tm1Ljb homozygotes does not occur in these mice

Allelic Composition	Il15^tm1Imx/Il15^tm1Imx Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il15^tm1Imx mutation (5 available); any Il15 mutation (43 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased CD8-positive, alpha-beta T cell number ( J:113408 )

• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in homozygote Itk^tm1Ljb thymus does not occur in these mice

• there is a 23-fold reduction in peripheral CD8⁺ T cells compared to wild-type mice and a 7-fold reduction compared to IL-15-sufficient Itk^tm1Ljb homozygotes

immune system

decreased CD8-positive, alpha-beta T cell number ( J:113408 )

• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in homozygote Itk^tm1Ljb thymus does not occur in these mice

• there is a 23-fold reduction in peripheral CD8⁺ T cells compared to wild-type mice and a 7-fold reduction compared to IL-15-sufficient Itk^tm1Ljb homozygotes

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tcrd^tm1Mom/Tcrd^tm1Mom
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tcrd^tm1Mom mutation (13 available); any Tcrd mutation (15 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:148531 )

N	• the hyper-IgE and increased numbers of germinal center B cells are at normal levels in these mice

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

Allelic Composition	H2^b/H2^b Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2^b mutation (28 available); any H2 mutation (280 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tg(TcrAND)53Hed mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

Allelic Composition	H2^b/H2^k Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2^b mutation (28 available); any H2 mutation (280 available)
H2^k mutation (15 available); any H2 mutation (280 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (48 available)
Tg(TcrAND)53Hed mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

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