Phenotypes associated with this allele

• Allele Symbol: Map3k3^tm1Bisu
• Allele Name: targeted mutation 1, Bing Su
• Allele ID: MGI:2178133
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Map3k3^tm1Bisu/Map3k3^tm1Bisu	Not Specified	MGI:3580326
cn2	Map3k3^tm1Bisu/Map3k3^tm2Bisu Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * CBA	MGI:3836810

Genotype
MGI:3580326

hm1

• Allelic Composition: Map3k3^tm1Bisu/Map3k3^tm1Bisu
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:60748 )

• by E11.5, all homozygotes were dead

• normal ratios of homozygotes found until E10

embryo

abnormal placental labyrinth vasculature morphology ( J:60748 )

• reduced number of embryonic blood vessels in the labyrinth layer of the placenta at E9.5

abnormal vitelline vascular remodeling ( J:60748 )

• yolk sac blood vessels lacking by E8 (15-20 somite stage)

• primordial blood plexus was present through E9.5 but dilated and with few red blood vessels

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:60748 )

• reduced number of embryonic blood vessels in the labyrinth layer of the placenta at E9.5

abnormal vascular development ( J:60748 )

• less development of blood vessels in the brain

• fewer and less complex intersomic blood vessel

decreased angiogenesis ( J:60748 )

• vasculogenesis was normal but subsequent remodeling failed

abnormal heart morphology ( J:60748 )

abnormal myocardium layer morphology ( J:60748 )

• myocardium development severely retarded

trabecula carnea hypoplasia ( J:60748 )

• decreased trabeculation

• less complex endocardium around trabeculations

abnormal heart development ( J:60748 )

• heart less developed

abnormal heart atrium morphology ( J:60748 )

• little atrial endocardium

muscle

abnormal myocardium layer morphology ( J:60748 )

• myocardium development severely retarded

trabecula carnea hypoplasia ( J:60748 )

• decreased trabeculation

• less complex endocardium around trabeculations

growth/size/body

microcephaly ( J:60748 )

• smaller head at E9.5

Genotype
MGI:3836810

cn2

• Allelic Composition: Map3k3^tm1Bisu/Map3k3^tm2Bisu; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

decreased CD4-positive, alpha-beta T cell number ( J:145923 )

• CD4⁺ T cell numbers are reduced by about half in the spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:145923 )

• CD8⁺ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type

spleen hypoplasia ( J:145923 )

• spleen size is significantly reduced mostly due to a reduction in the number of T cells

abnormal T cell physiology ( J:145923 )

• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:145923 )

• CD4⁺ T cell numbers are reduced by about half in the spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:145923 )

• CD8⁺ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type

spleen hypoplasia ( J:145923 )

• spleen size is significantly reduced mostly due to a reduction in the number of T cells

abnormal T cell physiology ( J:145923 )

• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice

cellular

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice