Phenotypes associated with this allele

• Allele Symbol: Egfr^tm1Wag
• Allele Name: targeted mutation 1, Erwin F Wagner
• Allele ID: MGI:1935108
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Egfr^tm1Wag/Egfr^tm1Wag	involves: 129	MGI:3765429
hm2	Egfr^tm1Wag/Egfr^tm1Wag	involves: 129 * C57BL/6	MGI:3765430
hm3	Egfr^tm1Wag/Egfr^tm1Wag	involves: 129 * C57BL/6 * MF1	MGI:3765431
ht4	Egfr^tm1Wag/Egfr^tm2(EGFR)Wag	involves: 129	MGI:2673651
ht5	Egfr^tm1Wag/Egfr^tm2(EGFR)Wag	involves: 129/Sv * C57BL/6	MGI:2673650

Genotype
MGI:3765429

hm1

• Allelic Composition: Egfr^tm1Wag/Egfr^tm1Wag
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:26834 )

• Background Sensitivity: no mice are found at E12.5 on a 129 only background while mice survive longer on a mixed 129 C57BL/6 background

growth/size/body

decreased embryo size ( J:26834 )

• at E10.5, embryos are slightly reduced in size compared to controls

decreased embryo weight ( J:26834 )

• at E10.5, embryos are slightly reduced in weight compared to controls

embryo

decreased embryo size ( J:26834 )

• at E10.5, embryos are slightly reduced in size compared to controls

decreased embryo weight ( J:26834 )

• at E10.5, embryos are slightly reduced in weight compared to controls

small placenta ( J:26834 )

Genotype
MGI:3765430

hm2

• Allelic Composition: Egfr^tm1Wag/Egfr^tm1Wag
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:26834 )

• Background Sensitivity: no mice survive until P20 unlike on a mixed 129 C57BL/6 MF1 background

lethality throughout fetal growth and development, incomplete penetrance ( J:26834 )

• fewer mice than expected mice develop to term

embryonic lethality during organogenesis, incomplete penetrance ( J:26834 )

• fewer than expected mice are present at E11.5 and fewer mice than expected mice develop to term

embryo

decreased embryo size ( J:26834 )

• at E13.5, embryos are slightly reduced in size compared to controls

decreased embryo weight ( J:26834 )

• at E13.5, embryos are slightly reduced in weight compared to controls

abnormal placenta morphology ( J:26834 )

• at E13.5, placentas are structurally abnormal

• however, the labyrinthine trophoblast cells and the network of embryonic vessels and maternal sinuses appear normal

abnormal maternal decidual layer morphology ( J:26834 )

♀ • at E13.5, the maternal deciduas separates from fetal components more easily than in wild-type mice

decreased spongiotrophoblast size ( J:26834 )

• the spongiotrphoblast layer is reduced in size compared to in wild-type mice

small placenta ( J:26834 )

respiratory system

abnormal bronchiole epithelium morphology ( J:26834 )

• mice display undifferentiated epithelium in the respiratory bronchioles

abnormal pulmonary alveolus epithelium morphology ( J:26834 )

• mice display undifferentiated epithelium in the alveoli

thick pulmonary interalveolar septum ( J:26834 )

• the number of cells in the alveolar septum is increased compared to in wild-type mice

vision/eye

decreased cornea thickness ( J:26834 )

• at birth, the cornea is thin and fibrotic

eyelids open at birth ( J:26834 )

• at E16.5, eyes are open and by birth eyelids have failed to grow and fused

growth/size/body

decreased embryo size ( J:26834 )

• at E13.5, embryos are slightly reduced in size compared to controls

decreased embryo weight ( J:26834 )

• at E13.5, embryos are slightly reduced in weight compared to controls

reproductive system

abnormal maternal decidual layer morphology ( J:26834 )

♀ • at E13.5, the maternal deciduas separates from fetal components more easily than in wild-type mice

integument

abnormal hair follicle morphology ( J:26834 )

• at birth some hair follicles appear less differentiated

• however, the number of hair follicles and organization of the dermal layer are similar to in wild-type mice

abnormal vibrissa morphology ( J:26834 )

• at birth, whiskers are absent or only rudimentary stubs are present

absent vibrissae ( J:26834 )

abnormal epidermal layer morphology ( J:26834 )

• at E18.5, epidermal layers are not distinct

abnormal epidermis stratum corneum morphology ( J:26834 )

• at E18.5, the stratum corneum lacks keratinization

thin epidermis ( J:26834 )

• at E18.5

Genotype
MGI:3765431

hm3

• Allelic Composition: Egfr^tm1Wag/Egfr^tm1Wag
• Genetic Background: involves: 129 * C57BL/6 * MF1

mortality/aging

postnatal lethality, incomplete penetrance ( J:26834 )

• fewer than expected mice survive until P20

homeostasis/metabolism

dehydration ( J:26834 )

• mice are severely dehydrated and suffer from wasting syndrome 2 days before death

growth/size/body

decreased body weight ( J:26834 )

• mice are 20% to 50% the size and weight of wild-type mice

cachexia ( J:26834 )

• mice are severely dehydrated and suffer from wasting syndrome 2 days before death

Genotype
MGI:2673651

ht4

• Allelic Composition: Egfr^tm1Wag/Egfr^tm2(EGFR)Wag
• Genetic Background: involves: 129

mortality/aging

prenatal lethality, complete penetrance ( J:84870 )

• complete penetrance

Genotype
MGI:2673650

ht5

• Allelic Composition: Egfr^tm1Wag/Egfr^tm2(EGFR)Wag
• Genetic Background: involves: 129/Sv * C57BL/6

mortality/aging

prenatal lethality, complete penetrance ( J:84870 )

• complete penetrance