Egfr^tm1Wag
Targeted Allele Detail

Summary

• Symbol: Egfr^tm1Wag
• Name: epidermal growth factor receptor; targeted mutation 1, Erwin F Wagner
• MGI ID: MGI:1935108
• Synonyms: EGFR-
• Gene: Egfr Location: Chr11:16702203-16868158 bp, + strand Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
• Alliance: Egfr^tm1Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:26834
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of the first exon was replaced by an in frame lacZ gene, followed by either a neomycin or a hygromycin selection cassette. These two different constructs were used to generate separate lines of mice, but the analysis did not distinguish between them. An EGFR-beta-galactosidase fusion protein was produced from this allele that was expressed under the control of the endogenous gene promoter. (J:26834)

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egfr Mutation: 85 strains or lines available

References

• Original: J:26834 Sibilia M, et al., Strain-dependent epithelial defects in mice lacking the EGF receptor [published erratum appears in Science 1995 Aug 18;269(5226):909]. Science. 1995 Jul 14;269(5221):234-8
• All: 10 reference(s)