Egfr^tm2(EGFR)Wag
Targeted Allele Detail

Summary

• Symbol: Egfr^tm2(EGFR)Wag
• Name: epidermal growth factor receptor; targeted mutation 2, Erwin F Wagner
• MGI ID: MGI:2673586
• Synonyms: hEGFR^KI
• Gene: Egfr Location: Chr11:16702203-16868158 bp, + strand Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
• Alliance: Egfr^tm2(EGFR)Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84870
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Egfr^tm2(EGFR)Wag expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EGFR (1956)

  Egfr	(MGI:95294)

• Mutation details: Floxed human cDNA encoding EGFR was inserted at the endogenous locus via homologous recombination. A RSV-neo cassette was inserted downstream of the human sequence. While endogenous levels of the human transcript were detected in brain tissue by RNAse protection, hypomorphic expression was observed in bone and epithelial tissue, putatively due to an effect of the downstream neo cassette. (J:84870)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egfr Mutation: 85 strains or lines available

References

• Original: J:84870 Sibilia M, et al., Mice humanised for the EGF receptor display hypomorphic phenotypes in skin, bone and heart. Development. 2003 Oct;130(19):4515-25
• All: 3 reference(s)