Phenotypes associated with this allele

• Allele Symbol: Socs1^tm1Wehi
• Allele Name: targeted mutation 1, Walter and Eliza Hall Institute of Medical Research
• Allele ID: MGI:1934607
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Socs1^tm1Wehi/Socs1^tm1Wehi	involves: 129S1/Sv * C57BL/6	MGI:2656950
cn2	Socs1^tm1Wehi/Socs1^tm3Wehi Tg(Lck-cre)I57Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * ICR	MGI:2656984
cx3	Prlr^tm1Cnp/Prlr^+ Socs1^tm1Wehi/Socs1^+	involves: 129P2/OlaHsd * 129S1/Sv * 129S2/SvPas * C57BL/6	MGI:4430639
cx4	Ifng^tm1Ts/Ifng^tm1Ts Socs1^tm1Wehi/Socs1^tm1Wehi	involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6	MGI:4430638
cx5	Ifng^tm1Ts/Ifng^+ Socs1^tm1Wehi/Socs1^tm1Wehi	involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6	MGI:4850158

Genotype
MGI:2656950

hm1

• Allelic Composition: Socs1^tm1Wehi/Socs1^tm1Wehi
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Phenotypic abnormalities in Socs1^tm1Wehi/Socs1^tm1Wehi mice

cellular

hepatic necrosis ( J:57474 )

• at P21

mortality/aging

decreased susceptibility to Togaviridae infection induced morbidity/mortality ( J:57474 )

• almost completely resistant to Semliki forest virus induced mortality

postnatal lethality, complete penetrance ( J:51281 , J:57474 , J:72528 )

• homozygotes become ill and die before reaching 3 weeks of age (J:51281)

• most die by 2 - 3 weeks of age (J:57474)

• raising mice in germ free conditions does not improve survival (J:57474)

• mice die from inflammatory disease before weaning (J:72528)

growth/size/body

decreased body weight ( J:51281 )

• homozygotes become smaller within 10 days after birth

postnatal growth retardation ( J:51281 )

hematopoietic system

hematopoietic system phenotype ( J:57474 )

N • treatment with an anti-IFNG antibody rescues most of the abnormalities

small thymus ( J:51281 )

thymus cortex atrophy ( J:51281 , J:57474 )

• the cortex becomes progressively depleted of lymphoid cells (J:51281)

• atrophy of the cortex (J:57474)

decreased bone marrow cell number ( J:57474 )

• lymphopenia in the bone marrow

decreased hematocrit ( J:51281 )

thrombocytopenia ( J:51281 )

• moderate reduction in platelet numbers

decreased leukocyte cell number ( J:57474 )

• in the bone marrow

decreased eosinophil cell number ( J:51281 )

• moderate reduction in eosinophil numbers

decreased lymphocyte cell number ( J:51281 )

decreased B cell number ( J:51281 )

• progressive loss of maturing B lymphocytes in the marrow, spleen, and peripheral blood

decreased pre-B cell number ( J:51281 )

• pre-B cells are depleted significantly in the marrow

abnormal mature B cell morphology ( J:51281 )

• number of mature B cells expressing surface Ig in the bone marrow is reduced

abnormal follicular B cell morphology ( J:51281 )

• lymphoid follicles of the spleen are either completely absent or are composed of immature cells

increased spleen red pulp amount ( J:51281 )

• most spleens show expanded areas of red pulp with nucleated erythroid cells as the main cell population

abnormal spleen white pulp morphology ( J:57474 )

• failure of lymphoid follicle development

abnormal macrophage physiology ( J:57474 )

• stimulation with as little as 0.01 U/ml IFNG is able to maintain efficient macrophage killing of Leishmania major parasites while concentration of less than 0.1 U/ml have little effect on wild-type macrophages

• increase in the capacity to produce NO

immune system

small thymus ( J:51281 )

thymus cortex atrophy ( J:51281 , J:57474 )

• the cortex becomes progressively depleted of lymphoid cells (J:51281)

• atrophy of the cortex (J:57474)

decreased leukocyte cell number ( J:57474 )

• in the bone marrow

decreased eosinophil cell number ( J:51281 )

• moderate reduction in eosinophil numbers

decreased lymphocyte cell number ( J:51281 )

decreased B cell number ( J:51281 )

• progressive loss of maturing B lymphocytes in the marrow, spleen, and peripheral blood

decreased pre-B cell number ( J:51281 )

• pre-B cells are depleted significantly in the marrow

abnormal mature B cell morphology ( J:51281 )

• number of mature B cells expressing surface Ig in the bone marrow is reduced

abnormal follicular B cell morphology ( J:51281 )

• lymphoid follicles of the spleen are either completely absent or are composed of immature cells

increased spleen red pulp amount ( J:51281 )

• most spleens show expanded areas of red pulp with nucleated erythroid cells as the main cell population

abnormal spleen white pulp morphology ( J:57474 )

• failure of lymphoid follicle development

abnormal macrophage physiology ( J:57474 )

• stimulation with as little as 0.01 U/ml IFNG is able to maintain efficient macrophage killing of Leishmania major parasites while concentration of less than 0.1 U/ml have little effect on wild-type macrophages

• increase in the capacity to produce NO

abnormal Peyer's patch morphology ( J:51281 )

• Peyer's patches are present but contain few lymphocytes

abnormal lymph node B cell domain morphology ( J:51281 )

• lymph nodes show absence of lymphoid follicle formation

abnormal inflammatory response ( J:57474 )

• infiltration of the liver, lungs, pancreas, heart, and skin with macrophages is seen by P21

increased inflammatory response ( J:72528 )

• mice die from massive generalized inflammation that affects multiple organs

heart inflammation ( J:51281 )

• monocytic, and less frequently, granulocytic infiltration

pancreas inflammation ( J:51281 )

• monocytic infiltration

liver inflammation ( J:51281 )

• livers exhibit both focal and generalized infiltration, predominantly by immature and mature monocytic and granulocytic cells, and less frequently, megakaryocytes and eosinophils

decreased susceptibility to Togaviridae infection induced morbidity/mortality ( J:57474 )

• almost completely resistant to Semliki forest virus induced mortality

liver/biliary system

hepatic necrosis ( J:57474 )

• at P21

liver inflammation ( J:51281 )

• livers exhibit both focal and generalized infiltration, predominantly by immature and mature monocytic and granulocytic cells, and less frequently, megakaryocytes and eosinophils

hepatic steatosis ( J:51281 , J:57474 )

• parenchymal cells contain an accumulation of lipid-containing vacuoles (J:51281)

• at P21 fatty degeneration of liver cells is seen (J:57474)

liver degeneration ( J:51281 )

• fatty degeneration either involves local areas not related to portal vessels or is generalized to the entire liver

cardiovascular system

heart inflammation ( J:51281 )

• monocytic, and less frequently, granulocytic infiltration

respiratory system

abnormal pulmonary alveolus wall morphology ( J:51281 )

• increase in cellularity of alveolar walls in the lung, often associated with macrophage cuffing around major vessels

endocrine/exocrine glands

small thymus ( J:51281 )

thymus cortex atrophy ( J:51281 , J:57474 )

• the cortex becomes progressively depleted of lymphoid cells (J:51281)

• atrophy of the cortex (J:57474)

pancreas inflammation ( J:51281 )

• monocytic infiltration

integument

thick epidermis ( J:57474 )

• thickening of the epithelium with keratinization at P21

Genotype
MGI:2656984

cn2

• Allelic Composition: Socs1^tm1Wehi/Socs1^tm3Wehi; Tg(Lck-cre)I57Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * ICR

immune system

enlarged thymus medulla ( J:83009 )

• enlarged thymic medulla

thymus hyperplasia ( J:83009 )

• increase in thymus cellularity predominately due to an increase in CD8+ T cell population

abnormal T cell differentiation ( J:83009 )

• enhanced differentiation of thymocytes toward CD8+ T cells

decreased B cell number ( J:83009 )

• large reduction in the percentage of non-T cells, mostly B cells, in the lymph nodes

abnormal T cell subpopulation ratio ( J:83009 )

• increase in the ratio of mature CD8 single positive:CD4 single positive thymocytes from 1:4 to 1:1

• increase in the ratio of CD8+:CD4+ T cells in the spleen, however total numbers of T cells are unchanged

abnormal CD4-positive, alpha beta T cell morphology ( J:83009 )

• mature thymocytes, both CD4 single positive and CD8 single positive, are larger in size

increased CD4-positive, alpha-beta T cell number ( J:83009 )

• increase in CD4+ T cells in the blood

abnormal CD8-positive, alpha beta T cell morphology ( J:83009 )

• mature thymocytes, both CD4 single positive and CD8 single positive, are larger in size

increased CD8-positive, alpha-beta T cell number ( J:83009 )

• large increase in the percentage and number of CD8 single positive thymocytes at 6 weeks of age

• increase in CD8+ T cells in the blood

abnormal T cell activation ( J:83009 )

• almost all peripheral CD8+ T cells but not CD4+ T cells appear as CD44^ho, indicating that they exhibit a memory rather than activated phenotype

enlarged lymph nodes ( J:83009 )

lymph node hyperplasia ( J:83009 )

• lymph nodes contain 2-fold the number of cells in controls

hematopoietic system

enlarged thymus medulla ( J:83009 )

• enlarged thymic medulla

thymus hyperplasia ( J:83009 )

• increase in thymus cellularity predominately due to an increase in CD8+ T cell population

abnormal T cell differentiation ( J:83009 )

• enhanced differentiation of thymocytes toward CD8+ T cells

decreased B cell number ( J:83009 )

• large reduction in the percentage of non-T cells, mostly B cells, in the lymph nodes

abnormal T cell subpopulation ratio ( J:83009 )

• increase in the ratio of mature CD8 single positive:CD4 single positive thymocytes from 1:4 to 1:1

• increase in the ratio of CD8+:CD4+ T cells in the spleen, however total numbers of T cells are unchanged

abnormal CD4-positive, alpha beta T cell morphology ( J:83009 )

• mature thymocytes, both CD4 single positive and CD8 single positive, are larger in size

increased CD4-positive, alpha-beta T cell number ( J:83009 )

• increase in CD4+ T cells in the blood

abnormal CD8-positive, alpha beta T cell morphology ( J:83009 )

• mature thymocytes, both CD4 single positive and CD8 single positive, are larger in size

increased CD8-positive, alpha-beta T cell number ( J:83009 )

• large increase in the percentage and number of CD8 single positive thymocytes at 6 weeks of age

• increase in CD8+ T cells in the blood

abnormal T cell activation ( J:83009 )

• almost all peripheral CD8+ T cells but not CD4+ T cells appear as CD44^ho, indicating that they exhibit a memory rather than activated phenotype

endocrine/exocrine glands

enlarged thymus medulla ( J:83009 )

• enlarged thymic medulla

thymus hyperplasia ( J:83009 )

• increase in thymus cellularity predominately due to an increase in CD8+ T cell population

Genotype
MGI:4430639

cx3

• Allelic Composition: Prlr^tm1Cnp/Prlr⁺; Socs1^tm1Wehi/Socs1⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129S2/SvPas * C57BL/6

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:70408 )

N • unlike in Prlr^tm1Cnp heterozygotes, lobuloalveolar structure development at 2 days postpartum is normal

reproductive system

reproductive system phenotype ( J:70408 )

N • unlike in Prlr^tm1Cnp heterozygotes, lobuloalveolar structure development at 2 days postpartum is normal

Genotype
MGI:4430638

cx4

• Allelic Composition: Ifng^tm1Ts/Ifng^tm1Ts; Socs1^tm1Wehi/Socs1^tm1Wehi
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6

mortality/aging

mortality/aging ( J:57474 , J:70408 )

N • unlike mice homozygous for Socs1^tm1Wehi alone, double homozygous mice survive past weaning and are overtly healthy (J:57474)

N • lethality observed in Socs1^tm1Wehi homozygotes is rescued (J:70408)

reproductive system

abnormal mammary gland growth during pregnancy ( J:70408 )

♀ • from day 16 of pregnancy, mice exhibit a higher density of lobuloalveolar units compared with wild-type mice

♀ • however, proliferation of mammary epithelium is normal

endocrine/exocrine glands

enlarged thymus medulla ( J:57474 )

• 8 of 12 mice have enlarged medulla

• however, unlike mice homozygous for Socs1^tm1Wehi alone, the cortex is normal

abnormal mammary gland growth during pregnancy ( J:70408 )

♀ • from day 16 of pregnancy, mice exhibit a higher density of lobuloalveolar units compared with wild-type mice

♀ • however, proliferation of mammary epithelium is normal

mammary gland alveolar hyperplasia ( J:70408 )

♀ • from day 16 of pregnancy, mice exhibit a higher density of lobuloalveolar units compared with wild-type mice

♀ • however, lobuloalveolar density returns to normal by day 5 of lactation

abnormal lactation ( J:70408 )

♀ • at day 18 of pregnancy, mammary glands produce more milk than in wild-type mice

cardiovascular system

abnormal lung vasculature morphology ( J:57474 )

• 2 of 12 mice show lymphoid cuffing of the lung vessels

hematopoietic system

hematopoietic system phenotype ( J:57474 )

N • unlike mice homozygous for Socs1^tm1Wehi alone, no hematological abnormalities are detected at 3 weeks of age

enlarged thymus medulla ( J:57474 )

• 8 of 12 mice have enlarged medulla

• however, unlike mice homozygous for Socs1^tm1Wehi alone, the cortex is normal

immune system

enlarged thymus medulla ( J:57474 )

• 8 of 12 mice have enlarged medulla

• however, unlike mice homozygous for Socs1^tm1Wehi alone, the cortex is normal

respiratory system

abnormal lung vasculature morphology ( J:57474 )

• 2 of 12 mice show lymphoid cuffing of the lung vessels

integument

abnormal mammary gland growth during pregnancy ( J:70408 )

♀ • from day 16 of pregnancy, mice exhibit a higher density of lobuloalveolar units compared with wild-type mice

♀ • however, proliferation of mammary epithelium is normal

mammary gland alveolar hyperplasia ( J:70408 )

♀ • from day 16 of pregnancy, mice exhibit a higher density of lobuloalveolar units compared with wild-type mice

♀ • however, lobuloalveolar density returns to normal by day 5 of lactation

abnormal lactation ( J:70408 )

♀ • at day 18 of pregnancy, mammary glands produce more milk than in wild-type mice

Genotype
MGI:4850158

cx5

• Allelic Composition: Ifng^tm1Ts/Ifng⁺; Socs1^tm1Wehi/Socs1^tm1Wehi
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6

mortality/aging

decreased survivor rate ( J:57474 )

• 8 of 12 mice died between 2 and 12 weeks of age, the remaining 4 survived

premature death ( J:57474 )

• 8 of 12 mice died between 2 and 12 weeks of age, the remaining 4 survived