Phenotypes associated with this allele

• Allele Symbol: Cxcr2^tm1Mwm
• Allele Name: targeted mutation 1, Mark Moore
• Allele ID: MGI:1857153
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	B6.Cg-Il8rb^tm1Mwm	MGI:3529063
hm2	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	C.129S2(B6)-Cxcr2^tm1Mwm/J	MGI:3514016
hm3	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	involves: 129S2/SvPas	MGI:5567832
hm4	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	involves: 129S2/SvPas * BALB/c	MGI:3687375
hm5	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	involves: 129S2/SvPas * C57BL/6J	MGI:3513832
ht6	Cxcr2^tm1Mwm/Cxcr2^+	C.129S2(B6)-Cxcr2^tm1Mwm/J	MGI:3514008
cx7	Dstn^corn1/Dstn^corn1 Cxcr2^tm1Mwm/Cxcr2^tm1Mwm	involves: 129S2/SvPas * A/WySn * BALB/c * brachy stock	MGI:3817227
cx8	Cxcr2^tm1Mwm/Cxcr2^tm1Mwm Tg(TRAMP)8247Ng/?	involves: 129S2/SvPas * BALB/c * C57BL/6	MGI:3694968

Genotype
MGI:3529063

hm1

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: B6.Cg-Il8rb^tm1Mwm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

decreased metastatic potential ( J:88233 )

• at 4 weeks, homozygotes show a significant reduction in spontaneous lung metastases of heterotopic LCC tumors relative to wild-type

abnormal tumor morphology ( J:88233 )

• in homozygotes, heterotopic LLC tumors display increased tumor necrosis relative to wild-type controls

• at 4 weeks, heterotopic LCC tumors from mutant mice exhibit reduced tumor angiogenesis but no differences on intratumor leukocyte infiltration relative to tumors from wild-type mice

decreased tumor growth/size ( J:88233 )

• homozygotes exhibit inhibition of both heterotopic and orthotopic Lewis lung cancer (LLC) primary tumor growth over 4 weeks relative to wild-type

Genotype
MGI:3514016

hm2

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: C.129S2(B6)-Cxcr2^tm1Mwm/J

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:85647 )

• in a model of septic peritonitis provoked by cecal ligation and puncture (CLP), homozygotes are significantly protected from CLP-induced tissue injury and mortality relative to wild-type mice

• in this model, enhanced survival is associated with higher resting and CLP-induced levels of peritoneal CXCL10

immune system

abnormal leukocyte physiology ( J:94170 )

• chemokine (C-X-C motif) ligand 5 (CXCL5; GCP-2) has little or no effect on resting wild-type or mutant lymphocytes

• however, CXCL5 significantly increases the expression of CD28 by CD3epsilon-stimulated lymphocytes from wild-type but not from mutant mice

• similar to the induction of CD28 expression, CXCL5 modestly increases the expression of CD80 and CD86 by B220+ B cells from wild-type but not from mutant mice costimulated by anti-CD3epsilon, mAb-treated T cells in culture

abnormal eosinophil physiology ( J:73947 )

• after conidial challenge, A. fumigatus-sensitized homozygotes exhibit impaired eosinophil recruitment into the airways relative to wild-type

abnormal neutrophil physiology ( J:73947 , J:93946 )

• at day 3 after conidia challenge, A. fumigatus-sensitized mutants display increased neutrophil activation (based on MPO levels) relative to wild-type (J:73947)

• surprisingly, neutrophil recruitment into the airways of sensitized mutants is comparable with that of sensitized wild-type mice (J:73947)

• TNF-treated homozygotes retain normal levels of neutrophil arrest in inflamed venules relative to BALB/c wild-type mice; however, treatment with anti-E-selectin mAb reduces neutrophil adhesion to approximately baseline levels (J:93946)

impaired neutrophil chemotaxis ( J:65905 , J:72824 )

• in response to infection with uropathogenic E. coli, mutant neutrophils fail to cross the epithelial barrier, accumulate in the tissues, and eventually cause renal scarring (J:65905)

• mutant neutrophils fail to migrate into the peritoneal cavity during early infection with Toxoplasma gondii; tachyzoite numbers are increased and PMN influx remains defective 36 h post-infection (J:72824)

impaired neutrophil recruitment ( J:63935 , J:65425 , J:93116 )

• homozygotes subjected to excisional wounds exhibit a significantly diminished neutrophil recruitment into the wound site, as shown by both myeloperoxidase assay and cell count (J:63935)

• in response to intravesical inoculation with E. coli, homozygotes display delayed neutrophil recruitment and subepithelial neutrophil accumulation during the first 7 days post-infection; neutrophils fail to cross the epithelium into the urine and neutrophil numbers excreted into the urine remain low at all times (J:65425)

• in response to infection with Pneumocystis sp., homozygotes exhibit a significantly reduced accumulation of neutrophils in the alveolar compartments (only 5-10% of wild-type); however, no major differences in pulmonary pathology are observed (J:93116)

increased IgE level ( J:73947 )

• A. fumigatus-sensitized homozygotes show significantly increased serum levels of IgE at days 7 and 37 after conidia, but not at any other times during the course of chronic fungal asthma

• notably, IgG1 levels remain unaffected at all times after conidia challenge

abnormal T cell physiology ( J:73947 )

• after conidial challenge, A. fumigatus-sensitized homozygotes display impaired T cell, but not neutrophil, recruitment into the airways relative to wild-type

abnormal T-helper 1 physiology ( J:73947 )

• A. fumigatus-sensitized homozygotes show significant increases in various Th1-associated chemokines and cytokines (IP-10/CXCL10, MIG/CXCL9, and IFN-gamma) at various times after conidia challenge

abnormal T-helper 2 physiology ( J:73947 )

• unlike wild-type mice, A. fumigatus-sensitized homozygotes fail to exhibit a robust Th2 response after conidia challenge

abnormal NK T cell physiology ( J:66399 )

• after intraocular OVA inoculation, homozygotes show no accumulation of NKT cells in their spleens and are unable to generate Ag-specific T regulatory cells; peripheral tolerance is thus prevented

• untreated homozygotes display reduced numbers of splenic NKT cells relative to wild-type mice, suggesting impaired development or basal trafficking of NKT cells to lymphoid organs

abnormal chemokine level ( J:73947 )

• after conidia challenge, whole lung levels of eotaxin/small chemokine (C-C motif) ligand 11 are significantly reduced in A. fumigatus-sensitized homozygotes relative to wild-type

abnormal interleukin level ( J:73947 , J:87365 )

• after conidia challenge, whole lung levels of IL-4 and IL-5 are significantly reduced in A. fumigatus-sensitized homozygotes relative to wild-type (J:73947)

• whole lung levels of IL-12 are reduced at all time points after conidia challenge; however, these differences become significant only at days 7 and 37 post-challenge (J:73947)

• levels of both mRNA and IL-6 protein levels in HSV-infected corneas of mutant mice are higher (up to 100-fold) than those in wild-type infected corneas (J:87365)

abnormal cytokine secretion ( J:72824 )

• homozygotes infected with Toxoplasma gondii show reduced production of proinflammatory cytokines, as shown by lowered TNF and IFN-gamma responses in spleen

decreased susceptibility to induced arthritis ( J:142880 )

• there is a signifcant decrease in clinical measurements of joint inflammation induced by Tg(TcraR28,TcrbR28)KRNDim (i.e. K/BxN) mouse serum compared to C57BL/6 controls

• ankle thickness is also half that of controls 9 days after arthritis induction

increased susceptibility to bacterial infection ( J:65905 )

• in response to infection with uropathogenic E. coli, homozygotes show an intact initial chemokine production, but display a diffuse CXCL2 (MIP-2) distribution at later time points

• at 7 days post-infection, homozygotes show impaired clearance of bacteria and acute pyelonephritic changes (i.e. edema, increased renal size, hyperemia, neutrophil influx, and abscess formation); some animals die of systemic infection

• at 35 days post-infection, kidneys of surviving mutants are pale and show parenchymal thinning, loss of cortical tissue, abscesses, fibrosis and diffuse inflammatory infiltrates

decreased susceptibility to fungal infection ( J:73947 )

• homozygotes sensitized to soluble A. fumigatus antigens are not susceptible to the lethal effects of a conidia challenge

• in contrast to wild-type, A. fumigatus-sensitized homozygotes fail to exhibit persistent airway signs of chronic fungal asthma

• no peribronchial inflammation, goblet cell hyperplasia, or fungal overgrowth is observed

increased susceptibility to parasitic infection ( J:72824 )

• homozygotes exhibit an increased susceptibility to infection with Toxoplasma gondii, associated with rapid tachyzoite infection and replication

• at 30 days post-infection, mutant brains harbor ~5-fold greater cyst numbers than wild-type infected mice

increased susceptibility to Herpesvirales infection ( J:87365 )

• homozygotes are more susceptible to HSV-induced ocular lesions, show impaired viral clearance, and develop severe herpetic stromal keratitis upon exposure to a dose of HSV that is minimally pathogenic to BALB/c wild-type mice

respiratory system

abnormal respiratory mucosa goblet cell morphology ( J:82293 )

• RSV-infected homozygotes display reduced goblet cell hyperplasia and a notable suppression in mucus production (based on decreased PAS+ staining and mucus in the BALF) relative to RSV-infected wild-type mice

abnormal airway responsiveness ( J:73947 )

• at days 3 and 7 after conidia challenge, A. fumigatus-sensitized homozygotes show significantly increased methacholine-induced airway hyperreactivity than wild-type mice

• in contrast, mutants display significantly reduced airway hyperresponsiveness than wild-type mice at days 14 and 37 after conidia

decreased airway responsiveness ( J:82293 )

• unlike wild-type mice, homozygotes fail to develop respiratory syncytial virus (RSV)-induced airway hyperresponsiveness after a methacholine challenge at all time points tested

vision/eye

corneal vascularization ( J:87365 )

• in HSV-infected corneas of mutant mice, abnormal IL-6 response is associated with enhanced corneal neovascularization (VEGFA induction)

nervous system

decreased oligodendrocyte progenitor number ( J:78470 )

• at P7, homozygotes exhibit fewer differentiated spinal cord oligodendrocytes, despite normal migration to the ventral presumptive white matter

• the remaining oligodendrocytes are abnormally displaced to the pial surface of the spinal cord

• reduced oligodendrocyte number is associated with decreased precursor proliferation in the white matter, and is partially compensated by decreased cell death

abnormal spinal cord morphology ( J:78470 )

• in homozygotes, developing spinal cords contain reduced oligodendrocytes, abnormally localized at the periphery

abnormal myelination ( J:78470 )

• in wild-type mice, myelin is distributed uniformly throughout the developing spinal cord white matter; in contrast, in mutants, myelin is concentrated at the periphery and deeper regions of white matter contain few myelin sheaths

homeostasis/metabolism

abnormal chemokine level ( J:73947 )

• after conidia challenge, whole lung levels of eotaxin/small chemokine (C-C motif) ligand 11 are significantly reduced in A. fumigatus-sensitized homozygotes relative to wild-type

abnormal interleukin level ( J:73947 , J:87365 )

• after conidia challenge, whole lung levels of IL-4 and IL-5 are significantly reduced in A. fumigatus-sensitized homozygotes relative to wild-type (J:73947)

• whole lung levels of IL-12 are reduced at all time points after conidia challenge; however, these differences become significant only at days 7 and 37 post-challenge (J:73947)

• levels of both mRNA and IL-6 protein levels in HSV-infected corneas of mutant mice are higher (up to 100-fold) than those in wild-type infected corneas (J:87365)

delayed wound healing ( J:63935 )

• homozygotes exposed to excisional punch biopsy show a delayed cutaneous healing response

• mutants show impaired neovascularization, reduced neutrophil recruitment, abnormal monocyte recruitment, and decreased secretion of IL-1beta into the wound bed

• also, primary cultures of mutant keratinocytes exhibit a delayed in vitro wound closure relative to wild-type, indicating a defective migration and proliferative response to wounding

skeleton

decreased susceptibility to induced arthritis ( J:142880 )

• there is a signifcant decrease in clinical measurements of joint inflammation induced by Tg(TcraR28,TcrbR28)KRNDim (i.e. K/BxN) mouse serum compared to C57BL/6 controls

• ankle thickness is also half that of controls 9 days after arthritis induction

cardiovascular system

corneal vascularization ( J:87365 )

• in HSV-infected corneas of mutant mice, abnormal IL-6 response is associated with enhanced corneal neovascularization (VEGFA induction)

cellular

decreased oligodendrocyte progenitor number ( J:78470 )

• at P7, homozygotes exhibit fewer differentiated spinal cord oligodendrocytes, despite normal migration to the ventral presumptive white matter

• the remaining oligodendrocytes are abnormally displaced to the pial surface of the spinal cord

• reduced oligodendrocyte number is associated with decreased precursor proliferation in the white matter, and is partially compensated by decreased cell death

impaired neutrophil chemotaxis ( J:65905 , J:72824 )

• in response to infection with uropathogenic E. coli, mutant neutrophils fail to cross the epithelial barrier, accumulate in the tissues, and eventually cause renal scarring (J:65905)

• mutant neutrophils fail to migrate into the peritoneal cavity during early infection with Toxoplasma gondii; tachyzoite numbers are increased and PMN influx remains defective 36 h post-infection (J:72824)

hematopoietic system

abnormal leukocyte physiology ( J:94170 )

• chemokine (C-X-C motif) ligand 5 (CXCL5; GCP-2) has little or no effect on resting wild-type or mutant lymphocytes

• however, CXCL5 significantly increases the expression of CD28 by CD3epsilon-stimulated lymphocytes from wild-type but not from mutant mice

• similar to the induction of CD28 expression, CXCL5 modestly increases the expression of CD80 and CD86 by B220+ B cells from wild-type but not from mutant mice costimulated by anti-CD3epsilon, mAb-treated T cells in culture

abnormal eosinophil physiology ( J:73947 )

• after conidial challenge, A. fumigatus-sensitized homozygotes exhibit impaired eosinophil recruitment into the airways relative to wild-type

abnormal neutrophil physiology ( J:73947 , J:93946 )

• at day 3 after conidia challenge, A. fumigatus-sensitized mutants display increased neutrophil activation (based on MPO levels) relative to wild-type (J:73947)

• surprisingly, neutrophil recruitment into the airways of sensitized mutants is comparable with that of sensitized wild-type mice (J:73947)

• TNF-treated homozygotes retain normal levels of neutrophil arrest in inflamed venules relative to BALB/c wild-type mice; however, treatment with anti-E-selectin mAb reduces neutrophil adhesion to approximately baseline levels (J:93946)

impaired neutrophil chemotaxis ( J:65905 , J:72824 )

• in response to infection with uropathogenic E. coli, mutant neutrophils fail to cross the epithelial barrier, accumulate in the tissues, and eventually cause renal scarring (J:65905)

• mutant neutrophils fail to migrate into the peritoneal cavity during early infection with Toxoplasma gondii; tachyzoite numbers are increased and PMN influx remains defective 36 h post-infection (J:72824)

impaired neutrophil recruitment ( J:63935 , J:65425 , J:93116 )

• homozygotes subjected to excisional wounds exhibit a significantly diminished neutrophil recruitment into the wound site, as shown by both myeloperoxidase assay and cell count (J:63935)

• in response to intravesical inoculation with E. coli, homozygotes display delayed neutrophil recruitment and subepithelial neutrophil accumulation during the first 7 days post-infection; neutrophils fail to cross the epithelium into the urine and neutrophil numbers excreted into the urine remain low at all times (J:65425)

• in response to infection with Pneumocystis sp., homozygotes exhibit a significantly reduced accumulation of neutrophils in the alveolar compartments (only 5-10% of wild-type); however, no major differences in pulmonary pathology are observed (J:93116)

increased IgE level ( J:73947 )

• A. fumigatus-sensitized homozygotes show significantly increased serum levels of IgE at days 7 and 37 after conidia, but not at any other times during the course of chronic fungal asthma

• notably, IgG1 levels remain unaffected at all times after conidia challenge

abnormal T cell physiology ( J:73947 )

• after conidial challenge, A. fumigatus-sensitized homozygotes display impaired T cell, but not neutrophil, recruitment into the airways relative to wild-type

abnormal T-helper 1 physiology ( J:73947 )

• A. fumigatus-sensitized homozygotes show significant increases in various Th1-associated chemokines and cytokines (IP-10/CXCL10, MIG/CXCL9, and IFN-gamma) at various times after conidia challenge

abnormal T-helper 2 physiology ( J:73947 )

• unlike wild-type mice, A. fumigatus-sensitized homozygotes fail to exhibit a robust Th2 response after conidia challenge

abnormal NK T cell physiology ( J:66399 )

• after intraocular OVA inoculation, homozygotes show no accumulation of NKT cells in their spleens and are unable to generate Ag-specific T regulatory cells; peripheral tolerance is thus prevented

• untreated homozygotes display reduced numbers of splenic NKT cells relative to wild-type mice, suggesting impaired development or basal trafficking of NKT cells to lymphoid organs

Genotype
MGI:5567832

hm3

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: involves: 129S2/SvPas

mortality/aging

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:208901 )

• resistant to fetal tuberculosis in M. tuberculosis-infected mice

immune system

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:208901 )

• resistant to fetal tuberculosis in M. tuberculosis-infected mice

Genotype
MGI:3687375

hm4

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: involves: 129S2/SvPas * BALB/c

growth/size/body

decreased body size ( J:113489 )

• female knockouts have slightly lower body weights than wild-type

increased kidney weight ( J:113489 )

• mice show a slight increase in kidney weight

increased spleen weight ( J:113489 )

• a significant increase (~165 mg) in spleen weight is observed compared to wild-type (95 mg)

hematopoietic system

decreased thymus weight ( J:113489 )

• male and female mice have slightly decreased thymus weights at 9 weeks

increased spleen weight ( J:113489 )

• a significant increase (~165 mg) in spleen weight is observed compared to wild-type (95 mg)

abnormal myelopoiesis ( J:113489 )

• a marked increase in extramedullary myelopoiesis is observed compared to wild-type

extramedullary hematopoiesis ( J:113489 )

• a moderate increase is observed vs wild-type

increased granulocyte number ( J:113489 )

• marked increase in absolute neutrophilic granulocyte count is seen in females

abnormal neutrophil physiology ( J:113489 )

• intradermal injection of human or mouse chemokines does not induce accumulation of neutrophils in the skin, in contrast to wild-type or knockin mice

immune system

decreased thymus weight ( J:113489 )

• male and female mice have slightly decreased thymus weights at 9 weeks

increased spleen weight ( J:113489 )

• a significant increase (~165 mg) in spleen weight is observed compared to wild-type (95 mg)

abnormal myelopoiesis ( J:113489 )

• a marked increase in extramedullary myelopoiesis is observed compared to wild-type

increased granulocyte number ( J:113489 )

• marked increase in absolute neutrophilic granulocyte count is seen in females

abnormal neutrophil physiology ( J:113489 )

• intradermal injection of human or mouse chemokines does not induce accumulation of neutrophils in the skin, in contrast to wild-type or knockin mice

reproductive system

increased seminal vesicle weight ( J:113489 )

♂ • males show a moderate increase in seminal vesicle weight

increased epididymis weight ( J:113489 )

♂ • males have slightly increased epididymis weight at 9 weeks

renal/urinary system

increased kidney weight ( J:113489 )

• mice show a slight increase in kidney weight

endocrine/exocrine glands

decreased thymus weight ( J:113489 )

• male and female mice have slightly decreased thymus weights at 9 weeks

increased seminal vesicle weight ( J:113489 )

♂ • males show a moderate increase in seminal vesicle weight

Genotype
MGI:3513832

hm5

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

hematopoietic system

hematopoietic system phenotype ( J:19570 )

N • despite myeloid hyperplasia of the marrow, homozygotes are not anemic, contain normal amounts of erythrocytes and hemoglobin, a normal hematocrit value, and do not display an increase in nucleated red blood cells

impaired neutrophil chemotaxis ( J:19570 )

• mutant neutrophils show a normal locomotor function and are effective at intracellular and extracellular killing of bacteria

• however, mutant neutrophils fail to chemotax in response to CXCL2 (MIP-2), and show impaired migration in response to thioglycollate injection; the number of mutant neutrophils that migrates to the peritoneum is one-fifth that of wild-type

enlarged spleen ( J:19570 )

• at necropsy, all homozygotes display a 2-4-fold increase in spleen size relative to wild-type

• in contrast, the thymus and all other organs remain grossly normal

abnormal myelopoiesis ( J:19570 )

• the presence of metamyelocytes, bands and neutrophils in normal ratios suggests that extramedullary myelopoiesis occurs in the liver, lymph node and spleen

abnormal granulocyte differentiation ( J:19570 )

• ~25% of homozygotes display multiple foci of granulopoiesis in the periportal region of the liver

• however, no signs of parenchymal infiltration, inflammation or hepatic damage are observed

myeloid hyperplasia ( J:19570 )

• homozygotes show a significant increase in bone marrow cellularity composed of the normal myeloid maturation series; the erythroid series remains unchanged

increased neutrophil cell number ( J:19570 )

• homozygotes show a ~12-fold increase in circulating neutrophils relative to wild-type

increased B cell number ( J:19570 )

• homozygotes display a ~10-fold increase in B cells relative to wild-type mice

abnormal spleen white pulp morphology ( J:19570 )

• spenomegaly results from expansion of the splenic white pulp by proliferation of myeloid elements (metamyelocytes, bands and neutrophils) and megakaryocytes

immune system

impaired neutrophil chemotaxis ( J:19570 )

• mutant neutrophils show a normal locomotor function and are effective at intracellular and extracellular killing of bacteria

• however, mutant neutrophils fail to chemotax in response to CXCL2 (MIP-2), and show impaired migration in response to thioglycollate injection; the number of mutant neutrophils that migrates to the peritoneum is one-fifth that of wild-type

enlarged spleen ( J:19570 )

• at necropsy, all homozygotes display a 2-4-fold increase in spleen size relative to wild-type

• in contrast, the thymus and all other organs remain grossly normal

abnormal myelopoiesis ( J:19570 )

• the presence of metamyelocytes, bands and neutrophils in normal ratios suggests that extramedullary myelopoiesis occurs in the liver, lymph node and spleen

abnormal granulocyte differentiation ( J:19570 )

• ~25% of homozygotes display multiple foci of granulopoiesis in the periportal region of the liver

• however, no signs of parenchymal infiltration, inflammation or hepatic damage are observed

myeloid hyperplasia ( J:19570 )

• homozygotes show a significant increase in bone marrow cellularity composed of the normal myeloid maturation series; the erythroid series remains unchanged

increased neutrophil cell number ( J:19570 )

• homozygotes show a ~12-fold increase in circulating neutrophils relative to wild-type

increased B cell number ( J:19570 )

• homozygotes display a ~10-fold increase in B cells relative to wild-type mice

abnormal spleen white pulp morphology ( J:19570 )

• spenomegaly results from expansion of the splenic white pulp by proliferation of myeloid elements (metamyelocytes, bands and neutrophils) and megakaryocytes

increased circulating interleukin-6 level ( J:19570 )

• homozygotes display significantly high serum levels of IL-6 (average of 4.8 ng/ml) relative to wild-type mice (below 0.1 ng/ml)

lymph node medullary cord hyperplasia ( J:19570 )

• in lymph nodes, the medullary cords are expanded by multiple foci of myelopoiesis, Russell bodies, and plasma cells, compressing the adjacent medullary sinuses

enlarged lymph nodes ( J:19570 )

• most mutant lymph nodes are enlarged; however, the degree of enlargement varies among individual mice

enlarged cervical lymph nodes ( J:19570 )

• all homozygotes exhibit enlarged cervical lymph nodes (3- to 10-fold)

• in contrast, mutant inguinal and popliteal lymph nodes appear grossly normal

skeleton

abnormal bone marrow morphology ( J:19570 )

• in homozygotes, the femur and tibia exhibit a grossly white marrow as opposed to the normal red marrow found in wild-type

homeostasis/metabolism

increased circulating interleukin-6 level ( J:19570 )

• homozygotes display significantly high serum levels of IL-6 (average of 4.8 ng/ml) relative to wild-type mice (below 0.1 ng/ml)

cellular

abnormal granulocyte differentiation ( J:19570 )

• ~25% of homozygotes display multiple foci of granulopoiesis in the periportal region of the liver

• however, no signs of parenchymal infiltration, inflammation or hepatic damage are observed

impaired neutrophil chemotaxis ( J:19570 )

• mutant neutrophils show a normal locomotor function and are effective at intracellular and extracellular killing of bacteria

• however, mutant neutrophils fail to chemotax in response to CXCL2 (MIP-2), and show impaired migration in response to thioglycollate injection; the number of mutant neutrophils that migrates to the peritoneum is one-fifth that of wild-type

growth/size/body

enlarged spleen ( J:19570 )

• at necropsy, all homozygotes display a 2-4-fold increase in spleen size relative to wild-type

• in contrast, the thymus and all other organs remain grossly normal

Genotype
MGI:3514008

ht6

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2⁺
• Genetic Background: C.129S2(B6)-Cxcr2^tm1Mwm/J

immune system

impaired neutrophil recruitment ( J:63935 )

• heterozygotes subjected to excisional wounds exhibit a diminished neutrophil recruitment into the wound site

• heterozygotes exhibit an intermediate myeloperoxidase (MPO) activity on postwound days 1 and 2 and an intermediate delay in neutrophil influx for postwound days 3-4 as compared with homozygotes or wild-type mice

• strikingly, heterozygotes exhibit a delayed increase in neutrophil count compared with wild-type mice; moreover, MPO activity does not correlate with neutrophil number on postwound day 7

homeostasis/metabolism

delayed wound healing ( J:63935 )

• heterozygotes exposed to excisional punch biopsy show a delayed cutaneous healing response that is intermediary relative to wild-type and homozygous mutant mice

hematopoietic system

impaired neutrophil recruitment ( J:63935 )

• heterozygotes subjected to excisional wounds exhibit a diminished neutrophil recruitment into the wound site

• heterozygotes exhibit an intermediate myeloperoxidase (MPO) activity on postwound days 1 and 2 and an intermediate delay in neutrophil influx for postwound days 3-4 as compared with homozygotes or wild-type mice

• strikingly, heterozygotes exhibit a delayed increase in neutrophil count compared with wild-type mice; moreover, MPO activity does not correlate with neutrophil number on postwound day 7

Genotype
MGI:3817227

cx7

• Allelic Composition: Dstn^corn1/Dstn^corn1; Cxcr2^tm1Mwm/Cxcr2^tm1Mwm
• Genetic Background: involves: 129S2/SvPas * A/WySn * BALB/c * brachy stock

vision/eye

abnormal cornea morphology ( J:139239 )

• compared to Dstn^corn1 homozygotes with at least one wild type allele of Il8rb, neutrophil recruitment to the cornea is almost completely inhibited; however, macrophages recruitment is still seen

corneal vascularization ( J:139239 )

• the decrease in neutrophil recruitment did not affect corneal vascularization

cardiovascular system

corneal vascularization ( J:139239 )

• the decrease in neutrophil recruitment did not affect corneal vascularization

Genotype
MGI:3694968

cx8

• Allelic Composition: Cxcr2^tm1Mwm/Cxcr2^tm1Mwm; Tg(TRAMP)8247Ng/?
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6

neoplasm

decreased tumor growth/size ( J:115957 )

♂ • tumor mass significantly less than in controls at 25 weeks of age

♂ • at 30 weeks of age, both tumor mass and volume are lower than in controls