Smurf1^tm1.1Kry
Targeted Allele Detail

Summary

• Symbol: Smurf1^tm1.1Kry
• Name: SMAD specific E3 ubiquitin protein ligase 1; targeted mutation 1.1, Gerard Karsenty
• MGI ID: MGI:8420547
• Synonyms: Smurf1^ki
• Gene: Smurf1 Location: Chr5:144813305-144902657 bp, - strand Genetic Position: Chr5, 84.83 cM, cytoband G2
• Alliance: Smurf1^tm1.1Kry page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235082
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details:

  Serine codon 148 (TCA) was changed to alanine (GCA) (p.S148A) and an FRT site flanked neomycin resistance gene cassette was inserted into an intron. The neo cassette was removed through subsequent Flp-mediated recombination. In the encoded protein, the mutation changes a phosphorylatable residue into one that cannot be phosphorylated, which affects osteoblast differentiation, bone formation, and glucose homeostasis. (J:235082)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smurf1 Mutation: 58 strains or lines available

References

• Original: J:235082 Shimazu J, et al., Smurf1 Inhibits Osteoblast Differentiation, Bone Formation, and Glucose Homeostasis through Serine 148. Cell Rep. 2016 Apr 5;15(1):27-35
• All: 1 reference(s)