Cpe^em1Yplo
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cpe^em1Yplo
• Name: carboxypeptidase E; endonuclease-mediated mutation 1, Y Peng Loh
• MGI ID: MGI:8385038
• Synonyms: CPE-E342Q
• Gene: Cpe Location: Chr8:65045576-65146088 bp, - strand Genetic Position: Chr8, 32.3 cM
• Alliance: Cpe^em1Yplo page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Glutamic acid codon 342 (GAG) in exon 6 was changed to glutamine (CAA) (p.E342Q) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation renders the encoded protein enzymatically inactive without affecting the neurotrophic activity. (J:388991)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpe Mutation: 26 strains or lines available

References

• Original: J:388991 Xiao L, et al., Neurotrophic factor-alpha1, a novel tropin is critical for the prevention of stress-induced hippocampal CA3 cell death and cognitive dysfunction in mice: comparison to BDNF. Transl Psychiatry. 2021 Jan 7;11(1):24
• All: 1 reference(s)