Adam5^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Adam5^em1Osb
• Name: a disintegrin and metallopeptidase domain 5; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:8347460
• Gene: Adam5 Location: Chr8:25217109-25314385 bp, - strand Genetic Position: Chr8, 13.26 cM
• Alliance: Adam5^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting of the intron between exons 4 and 5 and within exon 16 deleted exons 5 to 16. The 33,528 bp deletion was confirmed by genomic PCR and subsequent Sanger sequencing. Western blot analysis confirmed the absence of protein expression in the testes and spermatozoa of homozygous mutant males. (J:385037)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam5 Mutation: 55 strains or lines available

References

• Original: J:385037 Mashiko D, et al., ADAM5 is required for sperm-zona pellucida binding and sperm oviduct migration. Biol Reprod. 2026 Mar 16;114(3):1091-1100
• All: 1 reference(s)