Dctd^em1Boch
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dctd^em1Boch
• Name: dCMP deaminase; endonuclease-mediated mutation 1, Matthias Bochtler
• MGI ID: MGI:8261288
• Gene: Dctd Location: Chr8:48552127-48594702 bp, + strand Genetic Position: Chr8, 26.98 cM
• Alliance: Dctd^em1Boch page

Mutation origin

• Strain of Origin: C57BL/6JRj

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 218 bp deletion in the third coding (overall fourth) exon, with a 115 bp of the fragment inserted in an inverted orientation, causing a Tyr396fs*7 frameshift mutation. This allele was generated by coinjecting sgRNA targeting Dctd and Cdadc1 and generates a double knock-out with the Cdadc1 ^em2Boch allele. (J:372389)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dctd Mutation: 20 strains or lines available

References

• Original: J:372389 Slyvka A, et al., Activity and structure of human (d)CTP deaminase CDADC1. Proc Natl Acad Sci U S A. 2025 May 13;122(19):e2424245122
• All: 1 reference(s)