C2^em1Lgmo
Endonuclease-mediated Allele Detail

Summary

• Symbol: C2^em1Lgmo
• Name: complement C2; endonuclease-mediated mutation 1, Lori G Morton
• MGI ID: MGI:8224028
• Synonyms: C3^HET/C2 KO
• Gene: C2 Location: Chr17:35081578-35101076 bp, - strand Genetic Position: Chr17, 18.41 cM
• Alliance: C2^em1Lgmo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:369997
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology replaced exons 4-6 with a lacZ reporter self-deleting neomycin cassette in C3^tm1Lgmo ES cells in which the mouse C3 gene was replaced with a lacZ-loxP-neo-loxP cassette. This allele is a knock-out of both C2 and C3. (J:369997)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C2 Mutation: 30 strains or lines available

Notes

The allele was generated in 129 x C57BL/6 ES cells carrying C3^tm1Lgmo. (J:369997)

References

• Original: J:369997 Devalaraja-Narashimha K, et al., Humanized C3 Mouse: A Novel Accelerated Model of C3 Glomerulopathy. J Am Soc Nephrol. 2021 Jan;32(1):99-114
• All: 1 reference(s)