Gt(ROSA)26Sor^{tm5.1(CAG-Sun1/sfGFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5.1(CAG-Sun1/sfGFP)Nat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5.1, Jeremy Nathans
• MGI ID: MGI:6718511
• Synonyms: R26-CAG-LSL-Sun1-sfGFP-Myc, Rosa26^Sun1GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5.1(CAG-Sun1/sfGFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm5.1(CAG-Sun1/sfGFP)Nat} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sun1 (MGI:1924303)

• Mutation details: A targeting vector containing (from 5' to 3') a CAG promoter, a loxP site flanked STOP cassette, the mouse Sun1 cDNA fused with 2 copies of the superfolder GFP (sfGFP) green fluorescent protein gene and 6 copies of the Myc tag, and an FRT site flanked neomycin resistance gene cassette was inserted into the locus. Flp-mediated recombination removed the FRT-flanked neo cassette. Removal of the floxed stop cassette is required to achieve expression. (J:243557)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

Notes

The version of this allele that contains/ does not contain the neomycin selection cassette, Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat}, has been analyzed.

References

• Original: J:243557 Mo A, et al., Epigenomic Signatures of Neuronal Diversity in the Mammalian Brain. Neuron. 2015 Jun 17;86(6):1369-84
• All: 12 reference(s)