Rhof^tm1.2Dash
Targeted Allele Detail

Summary

• Symbol: Rhof^tm1.2Dash
• Name: ras homolog family member F (in filopodia); targeted mutation 1.2, Tadashi Matsushita
• MGI ID: MGI:6690835
• Synonyms: RhoF-, Rhof^tm1.1Mmar
• Gene: Rhof Location: Chr5:123256243-123279871 bp, - strand Genetic Position: Chr5, 62.77 cM
• Alliance: Rhof^tm1.2Dash page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303076
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 1 and a second loxP site into intron 2. Exon 2 was deleted through subsequent cre-mediated recombination. Immunoblots confirmed the absence of peptide expression from this allele in spleen, thymus and mesenteric lymph nodes. (J:303076)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rhof Mutation: 18 strains or lines available

References

• Original: J:303076 Kishimoto M, et al., Rhof promotes murine marginal zone B cell development. Nagoya J Med Sci. 2014 Aug;76(3-4):293-305
• All: 1 reference(s)