Phenotypes associated with this allele

• Allele Symbol: Rhof^tm1.2Dash
• Allele Name: targeted mutation 1.2, Tadashi Matsushita
• Allele ID: MGI:6690835
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rhof^tm1.2Dash/Rhof^tm1.2Dash	involves: 129	MGI:6690845

Genotype
MGI:6690845

hm1

• Allelic Composition: Rhof^tm1.2Dash/Rhof^tm1.2Dash
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:303076 )

N • normal thymus and spleen histology

N • normal numbers and kinetics of thymocytes and splenocytes

N • normal percentage and number of germline proB (BP1- CD24-), DJ rearranged-proB (BP1- CD24+) or early preB cells (BP1+ CD24+) in CD43+ B220+ bone marrow cells

N • normal percentage and number of late preB cells (IgD- IgM-), newly formed B cells (IgD- IgM+) or follicular-type recirculating B cells (IgD+ IgM+) in CD43- B220+ bone marrow cells

N • normal Transitional 1 (T1) (CD21- IgM+ CD23-), Transitional 2 (T2) ) (CD21+ IgM+ CD23+) and mature follicular (Fo) (CD21+ IgM- CD23+) B cells in spleen

N • normal immune response (IgM and IgG3 titers) to 2,4,6-trinitrophenyl-lipopolysaccharide (TNP-LPS)

N • normal migration of spleen marginal zone B cells towards B lymphocyte chemoattractant (BLC) and SDF-1alpha

abnormal spleen marginal zone morphology ( J:303076 )

• narrowing of B cell region (B220+ region outside CD169+ MZ macrophages)

decreased marginal zone B cell number ( J:303076 )

• reduction of percentage (in CD23- cells) and number of B cells (CD21+ IgM+ CD23-) in spleen

• normal Transitional 1 (T1) (CD21- IgM+ CD23-), Transitional 2 (T2) (CD21+ IgM+ CD23+) and mature follicular (Fo) (CD21+ IgM- CD23+) B cells

hematopoietic system

abnormal spleen marginal zone morphology ( J:303076 )

• narrowing of B cell region (B220+ region outside CD169+ MZ macrophages)

decreased marginal zone B cell number ( J:303076 )

• reduction of percentage (in CD23- cells) and number of B cells (CD21+ IgM+ CD23-) in spleen

• normal Transitional 1 (T1) (CD21- IgM+ CD23-), Transitional 2 (T2) (CD21+ IgM+ CD23+) and mature follicular (Fo) (CD21+ IgM- CD23+) B cells

growth/size/body

growth/size/body region phenotype ( J:303076 )

N • no gross abnormalities

embryo

embryo phenotype ( J:303076 )

N • born at normal Mendelian ratio

mortality/aging

mortality/aging ( J:303076 )

N • viable