Gt(ROSA)26Sor^{em1(CAG-Zfas1)Cya}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Zfas1)Cya}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Cyagen Biosciences
• MGI ID: MGI:6470593
• Synonyms: Gt(ROSA)26Sor^{em1(CAG-Zfas1)Cgen}, ZFAS1 TG
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Zfas1)Cya} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Zfas1)Cya} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Zfas1 (MGI:1916199)	floxed STOP cassette between CAG promoter and Zfas1 gene

• Mutation details: The following cassette was inserted into intron 1 using a gRNA with CRISPR/Cas9 technology: the CAG promoter, a loxP site flanked STOP cassette, the mouse Zfas1 gene, and poly(A) signal sequence. This creates a conditional-ready allele that will only express Zfas1 after cre-mediated removal of the floxed STOP cassette. (J:294533)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:294533 Jiao L, et al., lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca(2+) overload in myocardial infarction mice model. Cell Death Dis. 2019 Dec 9;10(12):942
• All: 1 reference(s)