Per2^em1Vrshp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Per2^em1Vrshp
• Name: period circadian clock 2; endonuclease-mediated mutation 1, David Virshup
• MGI ID: MGI:6453858
• Synonyms: PER2-S478A::LUC, S478A-Luc
• Gene: Per2 Location: Chr1:91343704-91387046 bp, - strand Genetic Position: Chr1, 46.13 cM, cytoband C5
• Alliance: Per2^em1Vrshp page

Mutation origin

• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing was used to insert TCC AGT to TCA GCT mutations in codons 477 and 478, resulting in the insertion of a serine to alanine point mutation in codon 478 (S478A). TGuide RNA, donor oligonucleotide, and the Cas9 nuclease were introduced into the cytoplasm of embryos derived from a cross of C57BL/6J males and Per2^tm1Jt-derived embryos with well recognized pronuclei. PCR amplicon sequencing was used to distinguish between the 129 and C57BL/6J alleles and founders with the S478A allele inserted in cis with Per2^tm1Jt in the 129 allele were selected. This allele includes the S478A mutation and a luciferase gene and floxed neo cassette inserted between exon 23 and the 3' UTR. (J:288606)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Per2 Mutation: 71 strains or lines available

References

• Original: J:288606 Masuda S, et al., Mutation of a PER2 phosphodegron perturbs the circadian phosphoswitch. Proc Natl Acad Sci U S A. 2020 May 19;117(20):10888-10896
• All: 1 reference(s)