Del(18Pcdha-Pcdhb)2Tyag
Targeted Allele Detail

Summary

• Symbol: Del(18Pcdha-Pcdhb)2Tyag
• Name: deletion, Chr 18, Takeshi Yagi 2
• MGI ID: MGI:6331337
• Synonyms: Del(18Pcdha-Pcdhb)1Tyag, Del2Tyag, Pcdhab^del
• Gene: Del(18Pcdha-Pcdhb)2Tyag Location: unknown Genetic Position: Chr18, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244163
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(18Pcdha-Pcdhb)2Tyag involves 38 genes/genome features (Pcdha, Pcdhb, Pcdha1 ...) View all
• Mutation details: This allele follows the meiotic cre-mediated recombination between the Pcdha^tm13Tyag and Pcdhb^tm2Tyag alleles. The Pcdha^tm13Tyag allele has a loxP and FRT site flanked neomycin resistance gene cassette followed by a myc-tagged Venus fluorescent protein gene cassette inserted into the 5' end of the first exon, alpha1, of the first gene in the cluster, Pcdha1. The Pcdhb^tm2Tyag allele has a loxP and FRT site flanked neomycin resistance gene cassette inserted downstream of the last gene, Pcdhb22, in the cluster. Recombination between the two results in the complete deletion of the Pcdha and Pcdhb clusters plus the Venus gene and the two neo cassettes. (J:244163)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(18Pcdha-Pcdhb)2Tyag Mutation: 0 strains or lines available

References

• Original: J:244163 Hasegawa S, et al., Distinct and Cooperative Functions for the Protocadherin-alpha, -beta and -gamma Clusters in Neuronal Survival and Axon Targeting. Front Mol Neurosci. 2016;9:155
• All: 2 reference(s)