Fbxw7^tm1Iken
Targeted Allele Detail

Summary

• Symbol: Fbxw7^tm1Iken
• Name: F-box and WD-40 domain protein 7; targeted mutation 1, Tsuneo Ikenoue
• MGI ID: MGI:6157075
• Synonyms: Fbxw7^LSL-R468C
• Gene: Fbxw7 Location: Chr3:84722575-84886505 bp, + strand Genetic Position: Chr3, 37.7 cM, cytoband E3.3
• Alliance: Fbxw7^tm1Iken page

Analysis of livers in mice with heterozygous and homozygous Fbxw7^tm1.1Iken mutations

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258194
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

Allele Type: Targeted (Conditional ready) Mutations: Insertion, Nucleotide substitutions Mutation details: Exons 6-10 were replaced with a construct containing exons 6 and 7, a loxP site, a minigene containing exons 8-11, a polyadenylation STOP sequence, a FRT-flanked neomycin resistance gene, a second loxP site, a mutated exon 8, and exons 9-10. The mutated exon 8 contains a C to T mutation at position 1402 and a G to T mutation at position 1404, resulting in an arginine to cysteine change at amino acid 468. The R468C mutation corresponds to the human R465C mutation, frequently detected in primary tumors. The mutant is expressed only after cre-mediated recombination. (J:258194)

Generation of the Fbxw7^tm1Iken and Fbxw7^tm1.1Iken alleles

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxw7 Mutation: 82 strains or lines available

References

• Original: J:258194 Ikenoue T, et al., Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018 Jan 31;8(1):2021
• All: 1 reference(s)