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| Caption | Establishment of conditional Fbxw7R468C knockin mice. (B) Schematic illustration of the targeting vector, and the wild-type Fbxw7, Fbxw7tm1Iken (Fbxw7LSL-R468C), and Fbxw7tm1.1Iken (Fbxw7R468C) alleles. The targeting vector contained the left (4.4 kb) and right (4.9 kb) arms consisting of exons 1-7 and exons 8-11, respectively, and a minigene comprising exons 8-11 of the mouse Fbxw7 and NeoR genes flanked by two FRT sites (grey triangles) in two loxP sites (black triangles) between the left and right arms. Exon 8 (closed red box in the right arm) harboured the mutations C1402T and G1404T (*). Cre-mediated recombination of the loxP sites resulted in the deletion of the mini gene and expression of the Fbxw7R468C mutant. The locations of genotyping primers (Fbxw7-RC-F and RC-R, denoted by arrowheads with F and R, respectively) and Southern blot probes are indicated. E: EcoRI sites, B: BamHI sites. | ||||||
| Copyright | This image is from Ikenoue T, Sci Rep 2018 Jan 31;8(1):2021, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:258194 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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