Cdr1os^em1Nikr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdr1os^em1Nikr
• Name: cerebellar degeneration related antigen 1, opposite strand; endonuclease-mediated mutation 1, Nikolaus Rajewsky
• MGI ID: MGI:6107169
• Synonyms: Cdr1as^-
• Gene: Cdr1os Location: ChrX:60159984-60247391 bp, + strand Genetic Position: ChrX, 34.19 cM
• Alliance: Cdr1os^em1Nikr page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The gene was deleted using the CRISPR/Cas9 system with two sgRNAs binding upstream of splice sites. The components were micro-injected into pronuclei of C57BL/6N zygotes. Lack of expression from the allele was confirmed by Northern blot and qRT-PCR analysis. (J:249957)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdr1os Mutation: 3 strains or lines available

References

• Original: J:249957 Piwecka M, et al., Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function. Science. 2017 Sep 22;357(6357)
• All: 2 reference(s)